Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0017536 (giardiasis)
 1,714 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This review describes the application of SEM to the study of microorganisms in gastrointestinal (GI) pathobiology. SEM has proven to be a highly useful tool with a variety of applications in the biological and medical sciences. Examples in this paper are focused on the use of secondary electron imaging to study selected bacterial (cholera), fungal (candidosis), and protozoan (giardiasis) diseases in the GI tract of murine (mouse and rat) experimental models. The successful application of SEM to studies of microorganisms in GI pathobiology requires strict attention to optimal preparative techniques, to selection of the appropriate method and microenvironment(s) for study, and to awareness of the indigenous microorganisms characteristic of the system studied.
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PMID:Application of scanning electron microscopy to the study of microorganisms in gastrointestinal pathobiology. 635 31

Mirazid -a purified oleo-resin extract derived from myrrh- with previously proved trematocidal activity, has been recently reported to be also effective against protozoa like Trichomonas vaginalis and cryptosporidium in humans. This experimental study aimed at investigating the possible therapeutic effect of mirazid on experimental Giardia lamblia infection in albino rats, using tinidazole as a therapeutic control. Results proved the efficacy of mirazid, as indicated by a 100% reduction in parasite- load of both intestinal and fecal parasitic counts, a direct toxic effect on Giardia trophozoite as revealed by SEM and TEM in form of cell membrane erosions together with increased cytoplasmic vaculation and dissociation, and also by reversal of the observed mucosal damage associated with Giardia after complete treatment. Starting trophozoite invasion of the mucosa was also demonstrated by TEM; its mechanism and possible pathogenic effect in giardiasis were discussed.
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PMID:Effect of mirazid (Commiphora molmol) on experimental giardiasis. 2163 51

Metronidazole (MTZ) has extremely broad spectrum of protozoal and antimicrobial activity and is clinically effective in trichomoniasis, amoebic colitis, and giardiasis. This study was performed to formulate and evaluate the MTZ loaded microspheres by coacervation phase separation and surface deposition and coalescence methods using different polymers like Gelatin, Carbopol 934P, Polylactic Acid (PLA), Eudragit RS30D, and Eudragit RL30D to acquire sustained release of drug. In vitro dissolution studies were carried out in phosphate buffer (pH 7.4) for 8 hours according to USP paddle method. The maximum and minimum release of MTZ from microspheres observed were 84.81% and 76.6% for coacervation and 95.07% and 80.07% for surface deposition method, respectively, after 8 hours. Release kinetics was studied in different mathematical release models. The SEM and FTIR studies confirm good spheres and smooth surface as well as interaction between drug and polymers. Though release kinetic is uncertain, the best fit was obtained with the Korsmeyer kinetic model with release exponent (n) lying between 0.45 and 0.89. In vitro studies showed that MTZ microspheres with different polymers might be a good candidate as sustained drug delivery system to treat bacterial infections.
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PMID:Surface Deposition and Coalescence and Coacervation Phase Separation Methods: In Vitro Study and Compatibility Analysis of Eudragit RS30D, Eudragit RL30D, and Carbopol-PLA Loaded Metronidazole Microspheres. 2664 28