Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0017536 (giardiasis)
 1,714 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Anti-Giardia antibodies were studied in 88 sera of patients with symptomatic and asymptomatic giardiasis by using the indirect immunofluorescent test and Giardia intestinalis trophozoites from an axenic culture as antigen. The sera of 40 healthy subjects were used as a control group and of 28 patients with other intestinal parasites (14 with Sarcocystis sp. and 14 with symptomatic Entamoeba histolytica infection) have been investigated for determination of test specificity. 82% of sera from patients with giardiasis presented G. intestinalis antibodies at titers of 1:16 to 1:256. Titers of at least 1:32, accepted as diagnostic and indicative of the infection, were found in 66% of patients with giardiasis. All of 18 patients with acute course of giardiasis had titers of at least 1:32. Only 5 sera (7%) from healthy control subjects or from patients infected with other intestinal parasites reacted at dilutions of 1: greater than or equal to 32. The indirect immunofluorescence test proves to be useful in detecting antibodies to G. intestinalis in the serum. However, the negative results occurring rather frequently in persons with chronic infection indicate a need for a more critical evaluation of serological tests in giardiasis.
 ...
PMID:Serum antibodies to Giardia intestinalis detected by immunofluorescence using trophozoites as antigen. 632 29

In summary, it appears that giardiasis, coccidiosis, cryptosporidiosis, strongyloidiasis, capillariasis and perhaps P. falciparum malaria are the only parasitic diseases which cause malabsorption of many nutrients. D. latum and A. lumbricoides interfere with vitamin B12 and vitamin A absorption, respectively. In view of the increasing use of immunosuppressive therapy, it is likely that malabsorption caused by intestinal parasites may become even more evident in the future.
 ...
PMID:Parasites and malabsorption. 640 70

In 1981, 900 children (aged 3 months to 10 years) and 146 staff attending 22 day-care centres in metropolitan Toronto chosen at random provided a stool specimen in a survey for intestinal parasites. Of the children, 4% to 36% were infected in 20 of 22 centres. Overall, 19% of the children and 14% of the staff had intestinal parasites: 8.6% and 4.0% respectively had Dientamoeba fragilis, and 7.8% and 2.0% respectively had Giardia lamblia. The highest prevalence of dientamebiasis was in the 7- to 10-year-olds, whereas giardiasis was detected most frequently in the 6-year-olds. Infection with intestinal parasites was not correlated with age, sex, duration in the day-care centre, dog ownership, travel history, gastrointestinal symptoms or the proportion of children in the day-care centre who were born in less developed countries. Immigrant children and children of parents born in industrialized countries (including Canada) were more likely to be infected than were children born in Canada of parents from the developing world. Dientamebiasis was associated with cat ownership. Thus, intestinal protozoa--in particular, D. fragilis and G. lamblia--are endemic in Toronto day-care centres.
 ...
PMID:Intestinal parasites in metropolitan Toronto day-care centres. 647 61

After oral administration of cysts of the intestinal protozoan parasite, Giardia muris, young male C3H/He mice are chronically infected, whereas BALB/c mice demonstrate a rapidly resolving pattern of infection. Both strains of mice injected with trophozoites in adjuvant and challenged orally with cysts develop serum antibodies to numerous trophozoite proteins. A limited number of these protein antigens was differentially immunoprecipitated by sera from resistant BALB/c and susceptible C3H/He mice exposed to G. muris. 35S-methionine-labelled protein antigens better recognized by immune BALB/c sera included molecules of relative mobility (Mr) 82,000 and a series of proteins of Mr 25,000 to 32,000. Differential recognition extended to a subset of solubilized trophozoite antigens that bind to the lectin, wheat germ agglutinin (WGA), and that can be radio-iodinated. In particular, a complex of 4 acidic protein antigens of approximate Mr 32,000, and designated collectively as Gm32, was better recognized by immune BALB/c serum than C3H/He serum. Isolated WGA-binding antigens were not able to consistently vaccinate BALB/c mice against subsequent G. muris infection. Moreover, preliminary evidence has been obtained that lack of antibody responsiveness to Gm32 does not segregate strictly with susceptibility to chronic infection in (BALB/c X C3H/He)F2 mice. These data, plus the observation that drug-cured C3H/He mice are highly resistant to reinfection, has led to examination of whether mice differ in the capacity of the intestines to support inflammatory responses. Mast cell deficient Wf/Wf mice, unlike wild-type litter-mates, developed chronic giardiasis although no reconstitution of resistance has yet been achieved with inocula of bone marrow cells from +/+ mice. BALB/c mice injected with the antihistamine and antiserotonin drug, cyproheptadine, also showed prolonged infections with G. muris. The data suggest that analysis of specificity differences in immune responses of mice varying in susceptibility to intestinal parasites must be supplemented by examination of the capacity of the intestine to support induced immune responses.
 ...
PMID:An examination of differences in serum antibody specificities and hypersensitivity reactions as contributing factors to chronic infection with the intestinal protozoan parasite, Giardia muris, in mice. 668 64

In response to public concerns, 165 Meo Laotians had stools screened for intestinal parasites by the Illinois Department of Public Health. One hundred twenty-nine had at least one pathogenic parasite detected. Hookworm was detected most frequently, followed by Giardia lamblia, Trichuris trichiura, and Ascaris lumbricoides. Hookworm and overall infection were more frequent in persons 4 years of age and older, while giardiasis, ascariasis, and trichuriasis were most common in the 4- to 14-year age group. Most infections were helminthic and of no public health consequence in the United States. However, giardiasis was seven times as prevalent in refugee children as in the general US population, posing a potential public health risk in child-care settings.
 ...
PMID:Intestinal parasites in Southeast-Asian refugees. Prevalence in a community of Laotians. 743 91

The reports about the frequency of intestinal protozooses found in humans who live in different localities of the Mexican Republic, are in general uncertain and not trustworthy, possibly because very few and poor epidemiological surveys have been undertaken in the country. However, with the few trustful studies carried out (1981 to 1991), it is possible in Mexico, to verify that amibiasis, giardiasis and criptosporidiosis are present with significant percentages of infection: 30.6, 22.3 and 39.3% respectively. With the summary of the researches analyzed in this article, one can conclude that human infections by intestinal protozoa in the Mexican Republic, at the present time, are almost as frequent as in past decades. This occurs because still remain the factors that contribute to the persistence and spreading of intestinal parasites, such as fecalism, poor hygienic and alimentary habits, within a deficient environmental sanitation.
 ...
PMID:[Frequency of intestinal protozoosis in the Mexican Republic]. 763 41

Antigen-capture enzyme-linked immunosorbent assay for the detection of Giardia lamblia-specific antigen in stool eluates from clinical subjects employing monoclonal antibody directed at 66-kDa G. lamblia copro-antigen has been evaluated. The G. lamblia copro-antigen was detected in 67% (31 of the 46 cases) of stool eluates from clinical cases, while none of the stool eluates from subjects with other intestinal parasites or from apparently healthy individuals, had detectable levels of G. lamblia copro-antigen. Monoclonal antibodies secreted by clones B4C5 and D3F4 recognised the periodate-sensitive and -insensitive epitopes of 66-kDa G. lamblia specific copro-antigen, respectively. Eight (73%) of the 11 symptomatic cases of giardiasis had trypsin-/periodate-sensitive epitopes of 66-kDa copro-antigen while 9 (92%) of 11 of the symptomatic cases and asymptomatic G. lamblia cyst carriers had trypsin-sensitive periodate-insensitive G. lamblia specific copro-antigen. The data tend to suggest that detection of periodate-insensitive epitopes of G. lamblia copro-antigen would indicate the presence of the parasite while the detection of periodate sensitive epitopes of G. lamblia copro-antigen would suggest symptomatic active giardial infection.
 ...
PMID:Uses and limitations of monoclonal antibodies to Giardia lamblia-specific 66-kDa copro-antigen in copro-immunodiagnosis of giardiasis. 768 67

Infection with intestinal parasites is a common problem among poor, urban populations in African countries and the Middle East. The authors assessed the prevalence of infection with intestinal parasites among children younger than five years old in an urban community in Khartoum and the factors involved. The community-based prospective study was conducted from March 1990 to February 1991. Soldiers and their families comprise a total of 25,400 individuals residing in ten camps in different areas of Khartoum. Each family has a two-room brick house with kitchen, piped water, and a pit latrine. Most families, however, have no refrigerator, so food is prepared daily and kept in covered containers. Drinking water is kept in large clay pots. 298 stool specimens were examined from 300 randomly selected children under five years old from three police force residential camps in Khartoum representing a total population of 4962 individuals. 116 of the samples were positive for a single parasite, while samples from 15 children showed ova and cysts for two types of parasites, giving a prevalence rate of 44%. The most common infections were giardiasis (21.1%), taeniasis (10.4%), and enterobiasis (7.4%). Nonpathogenic E. coli, E. histolytica, and Taenia saginata were detected in 2.7%, 0.7%, and 1.7% of stool specimens, respectively. Children aged 3 years and older were the most affected group, with the infection rate highest among the illiterate, overcrowded, and large-sized families. Malnourished children comprised 9.4% of the study group, but no significant association was found between undernutrition and the overall prevalence of intestinal infestations, although Giardia lamblia significantly affected the undernourished group.
 ...
PMID:Prevalence and risk factors of parasitic infections among under-five Sudanese children: a community based study. 779 47

A survey was carried out among the rural and urban settlers and two tribal groups viz. Nicobarese and Onges, of Andaman and Nicobar islands. The survey covered preschool school aged children and adults. Out of the total 1,384 stool samples examined, 652 (47.1%) showed ova or cysts of one or more intestinal parasites. Among the preschool children, Nicobarese showed the highest overall prevalence rate (80.5%) followed by urban (46.7%) and rural (38.6%) preschool children. Ascaris lumbricoides was the commonest form of parasite encountered in all the groups of preschool children, followed by Trichuris trichura. While ascariasis and trichuriasis were more common among the urban children than in rural children, giardiasis was more common among the rural preschool children. The school age children among rural settlers showed an overall prevalence rate of 61.1% which was significantly higher than that among the rural preschool children. Among the school age children also, ascariasis was the commonest form of parasitosis followed by trichuriasis. The Nicobarese and Onge adults showed significantly higher overall prevalence rates (72.2%) and 71.1% vs 48.6%) compared to rural adults. In all the groups studied ascariasis was the commonest form of parasitosis except in Onges among whom trichuriasis and giardiasis were more common than ascariasis. Change in prevalence rates over age was studied among the rural settlers. While ascariasis and trichuriasis showed peak prevalence rates in school age children prevalence of giardiasis declined with increase in age from a peak in the preschool age group and prevalence rates of hook worm infestation continued increasing beyond school age.
 ...
PMID:Intestinal parasitic infestation among different population groups of Andaman and Nicobar islands. 905 49

It is known that fecal examination to detect Giardia lamblia cysts or trophozoites produces a high percentage of false-negative results. A commercially available immunoenzymatic assay (ProSpecT Giardia Microplate Assay, Alexon, Inc., BIOBRAS) to detect G. lamblia specific coproantigen was evaluated for the first time in Brazil. A total of 90 specimens were tested. Each specimen was first tested as unpreserved stool, and then it was preserved in 10% Formalin to be tested 2 months later. The assay was able to identify all the 30 positive patients (sensitivity = 100.0%) by visual or spectrophotometric examination in the unpreserved specimens and was negative in 57 of the 60 patients without G. lamblia (specificity = 95.0%). The assay identified 27 of the 30 positive patients (sensitivity = 90.0%) and was negative in 59 of the 60 negatives (specificity = 98.3%) in the preserved stools according to both readings. A marked difference was observed in the optical densities in both groups, preserved and unpreserved stools, when the G. lamblia-positive specimens were compared to the negative or positive for other intestinal parasites than G. lamblia. The assay seems a good alternative for giardiasis diagnosis, especially when the fecal examination was repeatedly negative and the patient presents giardiasislike symptoms.
 ...
PMID:Detection of a giardia lamblia coproantigen by using a commercially available immunoenzymatic assay, in belo horizonte, brazil 1052 32


<< Previous 1 2 3 4 5 Next >>