Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0017160 (
gastroenteritis
)
11,398
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Vibrio parahaemolyticus
, a Gram-negative halophilic bacterium is often associated with fish and fishery products, thus causing
gastroenteritis
in humans upon ingestion of contaminated food.
V. parahaemolyticus
has become a globally well-known pathogen with yearly reported cases in many countries. This study aimed to discover the antibiotic resistance patterns of
V. parahaemolyticus
as well as detect Carbapenem resistant isolates from marine and freshwater fish in Selangor. A total of 240 freshwater and marine fish samples collected from wet market and supermarket in Selangor were tested for the presence of
V. parahaemolyticus
. All the fish samples were determined positive for
V. parahaemolyticus
using conventional microbiological culture-based method. The
toxR
gene were detected via polymerase chain reaction (PCR) in 165/240 (69%) isolates. The two-virulence factor of
V. parahaemolyticus
, thermostable direct hemolysin (
tdh
) and
TDH
-related hemolysin (
trh
) was screened via PCR. As such, four isolates were
trh
+and none were
tdh
+. Majority of the isolates presented high resistance to ampicillin (88%), amikacin (64%), and kanamycin (50%). In addition, this study identified 19-imipenem resistant isolates isolated from freshwater and marine fish samples. Further analysis of these 19-imipenem resistant isolates revealed that the resistance toward imipenem was plasmid mediated after plasmid curing assay. The multiple antibiotics resistance index was >0.2 for 70% of the isolates. In summary, the results confirm the presence of
V. parahaemolyticus
in freshwater and marine fish samples in Selangor, Malaysia. To our best knowledge, this is the first report discovering the antibiotic resistant patterns and Carbapenem-resistant isolates of
V. parahaemolyticus
isolated from marine and freshwater fish samples in Selangor.
...
PMID:Discovery on Antibiotic Resistance Patterns of
Vibrio parahaemolyticus
in Selangor Reveals Carbapenemase Producing
Vibrio parahaemolyticus
in Marine and Freshwater Fish. 3041 Apr 72
Seafood has frequently been associated with foodborne illness because pathogens are easily introduced during seafood cultivation, handling, and processing.
Vibrio parahaemolyticus
and
Vibrio cholerae
are human pathogens that cause
gastroenteritis
and cholera, respectively, and
Vibrio vulnificus
can cause fatal wound infections and septicemia. However, information about the occurrence of these pathogens in oysters from the Pacific coast of Mexico is limited to
V. parahaemolyticus
. In the present study, we evaluated the presence and abundance of these three
Vibrio
species in 68 raw oysters (
Crassostrea corteziensis
) obtained from retail seafood markets in Sinaloa, Mexico. The most probable number (MPN)-PCR assay was used for amplification of the
tlh
(thermolabile hemolysin),
ompW
(outer membrane protein), and
vvhA
(hemolytic cytolysin) genes that are specific to
V. parahaemolyticus, V. cholerae,
and
V. vulnificus,
respectively. All oyster samples were positive for at least one
Vibrio
species.
V. parahaemolyticus, V. cholerae,
and
V. vulnificus
prevalences were 77.9, 8.8, and 32.3% overall, respectively, and most species were present in all sample periods with increased prevalence in period 3. The
tdh
(thermostable direct hemolysin) gene was detected in 30.1%,
trh
(
TDH
-related hemolysin) was detected in 3.7%, and
tdh
/
trh
was detected in 7.5% of the total
tlh
-positive samples (53 of 68), whereas the pandemic serotype O3:K6 (
orf8
positive) was detected in only 1 sample (1.8%). The total prevalence of
tdh
and/or
trh
was 41.5%. In none of the samples positive for
V. cholerae
were the cholera toxin (
ctxA
) and cholix (
chxA
) toxigenic genes or the
rfb
gene encoding the O1 and O139 antigens amplified, suggesting the presence of non-O1 non-O139
V. cholerae
strains. Our results clearly indicated a high prevalence of pathogenic
Vibrio
species in raw oysters from retail seafood markets in Mexico. Consumption of these raw oysters carries the potential risk of foodborne illness, which can be limited by cooking.
...
PMID:Occurrence and Abundance of Pathogenic
Vibrio
Species in Raw Oysters at Retail Seafood Markets in Northwestern Mexico. 3172 80
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