Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0017160 (
gastroenteritis
)
11,398
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
SipA is a major effector of
Salmonella
, which causes
gastroenteritis
and enteric fever. Caspase-3 cleaves SipA into two domains: the C-terminal domain regulates actin polymerization, whereas the function of the N terminus is unknown. We show that the cleaved SipA N terminus binds and recruits host Syntaxin8 (Syn8) to
Salmonella
-containing vacuoles (SCVs). The SipA N terminus contains a SNARE motif with a conserved arginine residue like mammalian R-SNAREs. SipA
R204Q
and SipA
1-435R204Q
do not bind Syn8, demonstrating that SipA mimics a cognate
R-SNARE
for Syn8. Consequently,
Salmonella
lacking SipA or that express the SipA
1-435R204Q
SNARE mutant are unable to recruit Syn8 to SCVs. Finally, we show that SipA mimicking an
R-SNARE
recruits Syn8, Syn13, and Syn7 to the SCV and promotes its fusion with early endosomes to potentially arrest its maturation. Our results reveal that SipA functionally substitutes endogenous SNAREs in order to hijack the host trafficking pathway and promote
Salmonella
survival.
...
PMID:
Salmonella
SipA mimics a cognate SNARE for host Syntaxin8 to promote fusion with early endosomes. 3030 79
