Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0017160 (gastroenteritis)
 11,398 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Wetting of grids is the most important aspect of the detection of viruses by electron microscopy. Carbon or plastic/carbon coated grids used in most electron microscopy laboratories are negatively charged and hydrophobic (Sogo, Portman, Kaufman and Koller, 1975. J. Microsc. 104, 187-198.). Most viruses are negatively charged at pH 6.0-7.0 which does not allow them to adhere effectively to the support film. Viruses are then washed away or form clumps on the grid making detection more difficult. A suitable wetting agent, 0.1% poly-L-lysine hydrobromide (MW 35000) has been used for the detection of plant viruses (Roberts, personal communication). This chemical was used for processing faecal samples from patients with gastroenteritis for electron microscopy. The result showed a significant increase in terms of virus concentration on the grids and an increase in the general detection rate of positive samples. A study was then undertaken comparing the treatment method of grids using faecal samples. The grids were treated and not treated with poly-L-lysine hydrobromide.
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PMID:Detection of viruses by electron microscopy: an efficient approach. 967 37

Campylobacter jejuni has been isolated previously from market produce and has caused gastroenteritis outbreaks linked to produce. We have tested the ability of this human pathogen to utilize organic compounds that are present in leaf and root exudates and to survive in the plant environment under various conditions. Carbon utilization profiles revealed that C. jejuni can utilize many organic acids and amino acids available on leaves and roots. Despite the presence of suitable substrates in the phyllosphere and the rhizosphere, C. jejuni was unable to grow on lettuce and spinach leaves and on spinach and radish roots of plants incubated at 33 degrees C, a temperature that is conducive to its growth in vitro. However, C. jejuni was cultured from radish roots and from the spinach rhizosphere for at least 23 and 28 days, respectively, at 10 degrees C. This enteric pathogen also persisted in the rhizosphere of spinach for prolonged periods of time at 16 degrees C, a temperature at which many cool-season crops are grown. The decline rate constants of C. jejuni populations in the spinach and radish rhizosphere were 10- and 6-fold lower, respectively, than on healthy spinach leaves at 10 degrees C. The enhanced survival of C. jejuni in soil and in the rhizosphere may be a significant factor in its contamination cycle in the environment and may be associated with the sporadic C. jejuni incidence and campylobacteriosis outbreaks linked to produce.
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PMID:Comparison of survival of Campylobacter jejuni in the phyllosphere with that in the rhizosphere of spinach and radish plants. 1476 4