Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0017160 (gastroenteritis)
 11,398 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Rotaviruses are the major cause of severe dehydrating gastroenteritis in children worldwide. In this study, we report a positive role of cellular chaperone Hsp90 during rotavirus infection. A highly specific Hsp90 inhibitor, 17-allylamono-demethoxygeldanamycin (17-AAG) was used to delineate the functional role of Hsp90. In MA104 cells treated with 17-AAG after viral adsorption, replication of simian (SA11) or human (KU) strains was attenuated as assessed by quantitating both plaque forming units and expression of viral genes. Phosphorylation of Akt and NFkappaB observed 2-4 hpi with SA11, was strongly inhibited in the presence of 17-AAG. Direct Hsp90-Akt interaction in virus infected cells was also reduced in the presence of 17-AAG. Anti-rotaviral effects of 17-AAG were due to inhibition of activation of Akt that was confirmed since, PI3K/Akt inhibitors attenuated rotavirus growth significantly. Thus, Hsp90 regulates rotavirus by modulating cellular signaling proteins. The results highlight the importance of cellular proteins during rotavirus infection and the possibility of targeting cellular chaperones for developing new anti-rotaviral strategies.
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PMID:The molecular chaperone heat shock protein-90 positively regulates rotavirus infectionx. 1962 38

Arun P, Krishnasami K, Gunasekeran P, Fathima G, Padmanabhan V. Detection and molecular characterization of uncommon rotavirus group A genotype G12 among hospitalized children in Chennai. Turk J Pediatr 2019; 61: 209-216. Human group A (RVAs) possess genetic diversity and often newer RVA strains have been reported frequently with different G and P combinations worldwide. As the disease burden is more common in low income countries including India, monitoring and detection of the circulating strains of rotavirus needs to be monitored to understand the genetic diversity of the strains which evolve over time. This study was an attempt to provide data on rotavirus gastroenteritis prevalence in and around Chennai Tamilnadu-South India during the pre-vaccination era. Stool samples were collected and tested from 401 children less than five years of age admitted to hospitals with acute gastroenteritis. The samples were subjected to amplification for VP7 and VP4 genes by using consensus primers, followed by semi-nested type-specific multiplex PCR. Phylogenetic analysis of VP7 genes of G12 were carried out and the variations between strains isolated globally were documented. Rotavirus was detected in 167 (41.64%) samples and five were found to be the uncommon G12P[6] genotype (2.99%) tested by RT-PCR followed by G1P[8], G9P[4], G9 with P untypable and G3P[8] and combinations of G2G9P[6] and G9G2P[11] with few strains untypable. In this study we highlight the occurrence of uncommon G12P[6] strain of rotavirus infection in the community. Since rotavirus is transmitted through oral-fecal route and monitoring of environmental cleanliness is mandatory to cease the spread of this deadliest viral agent to achieve our MDG-IV. Covering single genotype will have to be modified with respect to the circulating stains.
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PMID:Detection and molecular characterization of uncommon rotavirus group A genotype G12 among hospitalized children in Chennai. 3195 30