Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0017160 (
gastroenteritis
)
11,398
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background:
Crohn's disease is one of the most significant intestinal disorders and is known as inflammatory bowel disease;
Campylobacter
spp. are one of the leading causes of bacterial
gastroenteritis
in humans.
Methods:
In this study, 60 tissue samples, including 30 cases with Crohn's disease and 30 cases with no inflammatory bowel disease, were collected. Patients were referred to Taleghani hospital and Behboud clinic between March 2015 and May 2016. Biopsies were used for DNA extraction and assessment of
Campylobacter jejuni
in patients with Crohn's disease and controls using polymerase chain reaction and quantitative real-time polymerase chain reaction. All positive amplified fragments were sequenced. The gene encoding 16S rRNA, specific to
Campylobacter
genus, was amplified.
Results:
The results were positive for
Campylobacter
genus in patients with Crohn's disease compared to healthy individuals. The quantitative real-time PCR showed a significantly higher prevalence of Campylobacter jejuni, particularly in
hippurate hydrolase
in tissue specimens of patients with Crohn's disease compared to control group. The correlation between
Campylobacter jejuni
and diarrhea symptoms in patients with Crohn's disease and controls was investigated. One positive case of
Campylobacter jejuni
found in patients without diarrhea was compared with 13 patients with diarrhea.
Conclusion:
The present study demonstrated the alarmingly high rate of
Campylobacter jejuni
prevalence in Crohn's disease patients with diarrhea symptoms. However, further investigation is needed to determine the possible causing factors of this disease.
...
PMID:Molecular detection of Campylobacter jejuni in patients with Crohn's disease in Iran. 3169 70
