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Query: UMLS:C0017160 (gastroenteritis)
 11,398 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Strains of transmissible gastroenteritis (TGE) virus possessing different pathogenicity were examined for stability to digestive enzymes and acid, and growth at various temperatures. In growth experiments, virus titer obtained at 37 degrees C were about equal between attenuated and virulent strains, but titers attained by the attenuated strain were higher at 30 degrees C. The attenuated virus multiplied at 28 degrees C, but the virulent virus did not at this temperature. The virulent virus was significantly stable to trypsin and pepsin, but the attenuated virus was inactivated rapidly by these proteolytic enzymes. No significant differences were observed in stability to acid between the attenuated and virulent strains. At different pH, both lost their infectivity more rapidly at 37 degrees C than at 22 degrees C.
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PMID:Comparison of properties between virulent and attenuated strains of transmissible gastroenteritis virus. 0 84

The influence of pH on the growth of transmissible gastroenteritis virus (TGEV) in adult pig thyroid cell culture, and on the stability of the virus was studied. At pH 7.2 and 100 fold higher than those at pH 8.0. The adsorption, penetration and uncoating steps of the viral replicative cycle were shown to be unaffected by pH variation. Synthesis of TGEV RNA during the first 12 hours post infection was found to be unaffected by pH variation between the range 6.5-8.0. After 12 hours breakdown of this RNA appeared to occur in cultures held at pH 7.2 and 8.0 but not at pH 6.5. When incubated at 37 degrees C for 24 hours the virus infectivity was found to be least affected by pH 6.5 but when kept at 4 degrees C for the same length of time, the virus infectivity remained constant between pH 5.0 and pH 8.0.
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PMID:The influence of pH on the growth and stability of transmissible gastroenteritis virus in vitro. 0 42

Characteristics of four transmissible gastroenteritis (TGE) virus field strains (Miller, Purdue, Bl, and V203) and four cell culture-attenuated strains (Purdue, SH, CKp, and Bl) were studied to find methods of differentiation between the two groups of viruses. TGE field virus strains did not replicate as well as attenuated strains at 37 C and could not be passaged serially for more than four to six passages at 33 C. There were clear differences in plaque size when the strains were compared. Field strains had average plaque sizes ranging from 3.59 to 3.15 mm, whereas attenuated strains induced plaques that were larger than 4.2 mm. Variations were observed in stability of strains at pH 3.0. Field strains and cell culture-attenuated strains CKp-270 and SH-114 were reduced in titer by about 1 log10. A reduction of about 3 log10, however, was obtained with cell culture strains B1-300 and Purdue-113.
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PMID:In vitro differentiation and pH sensitivity of field and cell culture-attentuated strains of transmissible gastroenteritis virus. 0 60

On occasion of an epidemic of acute nonbacterial gastroenteritis virus particles were visualized by electronmicroscopy in the faeces of 76 newborns; these particles had the following properties: mean diameter 28 nm; ether-resistent, stable at pH 3.0; buoyant density 1.33--1.34 g/ml in CsCl. About 10% of these particles had a definite star-like structure on their surface. Due to these properties the virusparticles were considered to be astroviruses. The virus was not cytopathogenic for cell cultures susceptible for enteroviruses, and not pathogenic for newborn mice.
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PMID:[Visualization by electronmicroscopy of 28 nm virusparticles (astroviruses) in faeces of newborns with acute nonbacterial gastroenteritis (author's transl)]. 3 52

A study of the health care delivery facilities in the Kainji Lake area of Nigeria (an artificial lake created in 1968) showed that hospitals, a health centre, maternal and child health centres, public health units, dispensaries and leper institutions, controlled by various organizations, are available. Dispensaries and leper settlements/clinics form the most numerous health providers in the rural areas. Analysis of 1973 data from eight dispensaries around Lake Kainji showed that malaria, gastroenteritis, chest and skin infections, venereal diseases and shistosomiasis constitute the major health problems. Observations of the environmental sanitation in the study area by the author support the idea that the diseases emanate particularly from the low standard of environmental health. A suggestion is made for the establishment of a central organization charged with the responsibilities for health planning and development. The evaluation of the impact of hte dispensaries as health providers is needed for future health planning. A health care delivery system supported by operational research should be initiated at the village level.
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PMID:A communication on health and development in the Kainji Lake area of Nigeria. 3 38

Methanol precipitation of transmissible gastroenteritis virus was tested at Ph 4.0, 5.0, 6.0, and 7.0 and at methanol concentrations of 15%, 25%, and 30%. Supernatant and precipitate fractions were tested for complement-fixing and agar-diffusion soluble antigens and plaque-forming units, and were examined by electron microscopy. Virus could be obtained free of detectable agar-diffusion antigens and most of the complement-fixing antigens. Most of the virions were without peplomers after methanol treatment but they retained infectivity.
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PMID:Methanol precipitation of transmissible gastroenteritis virus. 4 7

A 29 nm non-cultivable virus (NCV) was detected in faecal extracts from children hospitalized for gastroenteritis. The NCV had a density of 1.35 g/ml in glycerol-potassium tartrate density gradients and was resistant to degradation by proteolytic enzymes, non-ionic detergents and pH extremes. The surface of these virus particles had knob-like projections which appeared to have a symmetrical arrangement. When heated to 56 degrees C, the virus was completely degraded to soluble components which could not be seen by electron microscopy.
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PMID:Biophysical properties of a non-cultivable 29-nm enteric virus. 4 59

Two outbreaks of acute gastroenteritis occurred in 1974 in a long-stay children's ward. Electron microscopy demonstrated rotaviruses in faeces from the affected children in the first outbreak, and adenoviruses in faeces from affected children and a nurse in the second outbreak. The illness in both outbreaks was very mild; but the diarrhoea associated with rotavirus infection usually lasted 5-8 days (in one patient it lasted for 28 days) and sometimes started with vomiting; whereas the adenovirus-associated diarrhoea lasted only 2-4 days and was not associated with vomiting. Neither the rotaviruses nor the adenoviruses could be established in tissue-culture.
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PMID:Epidemic viral enteritis in a long-stay children's ward. 4 58

A complement-fixation (C.F.) test for the human reovirus-like agent of infantile gastroenteritis has been developed using the serologically related Nebraska calf diarrhoea virus (N.C.D.V.) as antigen. Most infants and children who shed the agent in stools and/or who demonstrated serological (C.F.) evidence of infection with a reovirus-like-particle-positive human stool-filtrate C.F. antigen also demonstrated serological evidence of infection when a concentrated N.C.D.V. preparation was employed AS C.F. antigen. The N.C.D.V., which was previously shown to be related to the human reovirus-like agent, was found to be related antigenically to the epizootic diarrhoea of infant mice (E.D.I.M.) virus also. Studies on the prevalence of C.F. antibody in sera from infants and young children revealed a pattern of rapid acquisition of antibody to both the human reovirus-like agent and the N.C.D.V. as over 80 percent of these individuals possessed antibody to each agent by 36 months of age. A strong positive association was found in the results obtained with the two antigens. The ready availability of cell-culture grown N.C.D.V., and its ability to serve as a "substitute" C.F. antigen for the human reovirus-like agent, should enable the serodiagnosis of many cases of disease due to the human agent and facilitate seroepidemiological studies of such infections. In addition, the observation that a large proportion of individuals infected with the human reovirus-like agent develop serological evidence of infection not only to the human agent but to the calf agent as well may have important implications in the immunoprophylaxis of disease caused by the human reovirus-like agent.
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PMID:New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. 4 29

Strains of Escherichia coli isolated from patients with suspected pyelonephritis had a strong predilection for growth in kidney tissue. Viable bacterial counts in experimentally infected mice showed that after 96 hours approximately 98% of the total-body count was found in the kidney. Strains of E. coli isolated from cases of gastroenteritis in man and animals had little or no tendency to grow in the kidney. Treatment of male and female mice with oestrogen significantly enhanced the growth of "pyelonephritic" strains in the kidney, but had no effect of any kind on the growth of "gastroenteritis" strains. These preliminary results suggest that oestrogen may predispose to the development of kidney infection in the female and that there is an important link with the virulence of the E. coli concerned. Only those strains that have a natural predilection for growth in the kidney are likely to be influenced by oestrogen.
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PMID:Influence of oestrogen on experimental pyelonephritis caused by Escherichia coli. 5 May 4


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