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Query: UMLS:C0016632 (Fox)
 1,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasmic membrane. When trypsinized whole cells (from which the M protein on the cell wall had been digested) or protoplasts were extracted with hot HCl, no M protein antigen was detected, although the antigen was routinely extracted from the cell walls of normal streptococci by the hot acid procedure. Various serological techniques, including the use of fluorescein-labeled type-specific antisera, were employed to demonstrate the M antigen in association with the membrane from osmotically lysed protoplasts.
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PMID:INTRACELLULAR M PROTEIN OF GROUP A STREPTOCOCCUS. 1404 30

During lyophilisation cycle design, primary drying parameters (chamber pressure and shelf temperature) are adjusted to maximize the sublimation rate and prevent cake collapse, by maintaining the product continuously below its critical temperatures. The objective of this study was to employ mixture design of experiments to generate empirical models capable of predicting glass transition of the maximally freeze concentrated solution (Tg') and collapse temperature (Tc) of amorphous protein (BSA and IgG1) formulations. Additionally, the models developed aid the design of high concentration protein formulations with maximised critical temperatures to obtain shorter and more cost-effective lyophilisation cycles. Formulations contain sucrose as cryo/lyo-protectant and arginine/arginine-HCl as multifunctional excipient (e.g. solubility enhancer, viscosity and aggregation suppressor). The impact of formulation components at varied ratios on critical temperatures was evaluated; the amorphous excipients decrease critical temperatures, on the contrary, the protein increases critical temperatures. The robustness of the empirical models generated with BSA formulations was verified with BSA and IgG1 formulations. The models showed greater accuracy in predicting Tg' than the Fox-Flory equation. For the first time, empirical models are reported to predict both critical temperatures. Finally, unconventional collapse events observed for formulations with and without arginine/arginine-HCl at different protein concentrations are also discussed.
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PMID:Application of a mixture DOE for the prediction of formulation critical temperatures during lyophilisation process optimisation. 3167 26