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Query: UMLS:C0016632 (Fox)
 1,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. T-, and L-type Ca2+ channels were studied in cell-attached patch recordings from the cell bodies of chick dorsal root ganglion neurones. All experiments were performed with isotonic BaCl2 (110 mM) in the recording pipette and with isotonic potassium aspartate in the bathing solution to zero the cell membrane potential. 2. L-type channels are distinguished by a unitary slope conductance of 25 pS, activation over the range of membrane potentials between 0 and +40 mV, little inactivation over the course of a 136 ms depolarization, and availability for opening even at depolarized holding potentials (h.p. greater than -40 mV). L channels show a predominant mode of gating (mode 1) characterized by brief openings (approximately 1 ms), occasionally interspersed with another pattern of gating characterized by much longer openings (mode 2). 3. The dihydropyridine (DHP) Ca2+ agonist Bay K 8644 promotes mode 2 activity and shifts the voltage dependence of L-type channel activation towards more negative potentials. It leaves the unitary current-voltage relation unchanged. 4. Nifedipine, a DHP Ca2+ antagonist, strongly inhibits L-type channel activity through an increase in the proportion of blank sweeps. 5. T-type Ca2+ channels are distinguished by a much smaller unitary slope conductance (8 pS) and by activation and inactivation over relatively negative ranges of potential. Inactivation is complete by the end of 136 ms pulses to test potentials beyond -20 mV. 6. N-type Ca2+ channels are distinguished by an intermediate unitary slope conductance (13 pS), and by activation over a range of potentials between those of T- and L-type channels. Inactivation of N-type channels takes place over an exceptionally broad range of holding potentials (-80 to -20 mV). 7. Cell-attached patch data on the voltage dependence of activation and inactivation of T- and N-type channels are in excellent agreement with results from whole-cell recordings (Fox, Nowycky & Tsien, 1987) if allowances are made for variations in external surface potential. 8. Patches containing one or two channels of a single type were used for analysis of gating kinetics. The predominant pattern of activity for each of the channel types is an exponential distribution of relatively brief (approximately 1 ms) openings, and a bi-exponential distribution of short and long closings. 9. Patches containing all possible combinations of channel types were observed. However, preliminary evidence suggests that channels are distributed unevenly over the cell body; clustering of N-type channels is particularly prominent.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Single-channel recordings of three types of calcium channels in chick sensory neurones. 245 Oct 17

1. Ca2+ channels were studied in cultured glomerulosa cells from the rat adrenal gland. The whole-cell configuration of the patch-clamp technique was used. Cs+-filled pipettes were used in order to block K+ channels. 2. Three Ca2+ components were found, namely, T, L and N, according to the nomenclature proposed by Nowycky, Fox & Tsien (1985). The T-component was a fast transient component activated in the range -60 to -40 mV; the L-component did not inactivate for a sustained depolarization and activated at voltages around -30 mV; the third component, the N-component, was transient and was activated at voltages close to -20 mV. 3. A statistical analysis made on seventy-one experiments showed that the L-component was the most frequent (65% of the experiments), followed by the T- and finally the N- components (59 and 29% of the experiments, respectively). 4. The substitution of Ba2+ ions for Ca2+ ions greatly enhanced the L-component's amplitude (iBa/iCa = 4) while the N-component was unaffected and the T-component was reduced (iBa/iCa = 0.4). 5. A comparison of the voltage-dependent steady-state inactivation of the three components showed that the T-component was inactivated at -60 mV while the inactivation of the L- and N-components was complete at -25 and 0 mV, respectively. 6. A run-down effect was detected in some cells. The time stability of the L-component was lower than that of the T-component. The N-component seemed to be insensitive for at least 1 h. The results for the L- and T-components were obtained in cells which presented no run-down of the current or only a weak one. 7. Cd2+ ions (5 x 10(-5)M) completely blocked the long-lasting component (L-component) and slightly decreased the T-component. 8. Bay K 8644, a dihydropyridine agonist, enhanced the L-component at a concentration of 2.5 microM but decreased it for a higher concentration (5 microM). The T-component was decreased in a reversible way by 1 microM-Bay K 8644. Nifedipine, a well-known antagonist, blocked completely the L-component. This effect was reversed by the addition of Bay K 8644 to the perfusion medium. The T-component was also blocked by nifedipine, a result which is in keeping with the fact that Bay K 8644 has a weak effect on this current.
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PMID:Three components of the calcium current in cultured glomerulosa cells from rat adrenal gland. 247 2

1. Ionic currents associated with the invasion of an action potential into the motor nerve ending of the lizard, Anolis carolinensis, were measured with a focal extracellular electrode at several locations along the nerve ending. 2. These experimentally observed currents could be matched with computer simulations of action potential propagation into the nerve ending. They revealed that while Na+ channels are the major ionic current pathway in the heminode, K+ channels provide the major pathway in the terminal branches and boutons. 3. Calcium current in the presynaptic ending was unmasked by the application of tetraethylammonium (TEA). This current was blocked by: (a) cadmium, (b) omega-conotoxin GVIA and (c) nifedipine, but was unaffected by nickel at concentrations less than or equal to 100 microM. Nifedipine's action became more definitive when the duration of the action potential was greatly extended by pre-treatment with TEA. The effect of Bay K 8644 was inconsistent. 4. Transmitter release, as measured by postsynaptic current, had a pharmacological response profile similar to that of the Ca2+ current, with the exception that transmitter release was increased reliably and reversibly by Bay K 8644. 5. This pharmacological response profile is identical to that of the L type Ca2+ channel identified by Fox, Nowycky & Tsien (1987 alpha) in chick dorsal root ganglion neurones. We saw no evidence for more than a single type of Ca2+ channel in lizard motor nerve endings. 6. A calcium-activated K+ current IK(Ca) was revealed by application of 3,4-diaminopyridine (DAP), a delayed-rectifier K+ channel blocker. This K(Ca) current was blocked by TEA, charybdotoxin and by substitution of cobalt for extracellular calcium.
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PMID:Identification of ionic currents at presynaptic nerve endings of the lizard. 257 61