Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0016632 (Fox)
 1,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

When grown under hypothermic conditions, CHO (Chinese-hamster ovary) cells become growth-arrested in the G0/G1 phase of the cell cycle and also often exhibit increased recombinant-protein production. We have shown in the accompanying paper [Fox, Tan, Tan, Wong, Yap and Wang (2005) Biotechnol. Appl. Biochem. 41, 255-264] that the positive effect of low temperature on recombinant-protein production is due to elevated mRNA levels and not due to G0/G1-phase growth arrest and that a cell line can still show growth-associated productivity at low temperature. This finding led to the hypothesis that improved total production of recombinant protein would be achieved by stimulating cells to actively grow at low temperature, a culture condition previously unreported in the literature. In the present study we have validated this hypothesis by stimulating hypothermic (32 degrees C) growth through the use of different growth factors. Hypothermic growth was stimulated in fetal-bovine-serum-supplemented adherent cultures using basic fibroblast growth factor or insulin. Hypothermic growth was also stimulated in suspension cultures normally grown in protein-free medium by using supplementation with fetal bovine serum. These methods resulted in up to 7.7- and 4.9-fold increases in total recombinant-protein production compared with the 37 and 32 degrees C control cultures respectively. This proof-of-concept study will motivate the creation of cell lines capable of growing at low temperatures for use in industrial processes.
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PMID:Active hypothermic growth: a novel means for increasing total interferon-gamma production by Chinese-hamster ovary cells. 1550 4

Medical records of 80 dogs diagnosed with acute pancreatitis during a 4-year period were evaluated regarding history, breed predilection, clinical signs and additional examination findings. Cases were selected if compatible clinical symptoms, increased serum activity of amylase or lipase and morphologic evidence of pancreatitis by ultrasonography, laparotomy or necropsy were all present. Like in other studies, neutered dogs had an increased risk of developing acute pancreatitis. Although breed predilection was consistent with earlier reports, some notable differences were also observed. Apart from Dachshunds, Poodles, Cocker Spaniels and Fox Terriers, the sled dogs (Laikas, Alaskan Malamutes) also demonstrated a higher risk for pancreatitis according to our results. Concurrent diseases occurred in 56 dogs (70%), diabetes mellitus (n = 29, 36%) being the most common. Clinical signs of acute pancreatitis were similar to those observed in other studies. The study group represented a dog population with severe acute pancreatitis, having a relatively high mortality rate (40%) compared to data of the literature. Breed, age, gender, neutering and body condition had no significant association with the outcome. Hypothermia (p = 0.0413) and metabolic acidosis (p = 0.0063) correlated significantly with poor prognosis and may serve as valuable markers for severity assessment in canine acute pancreatitis.
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PMID:Occurrence, clinical features and outcome of canine pancreatitis (80 cases). 2135 40