Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0016632 (Fox)
 1,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Increased utilization of the granulocyte-macrophage colony-forming unit (CFU-GM) assay for quality control, dosing, and clinical investigation of peripheral blood (PB) stem cell and bone marrow (BM) products led us to compare two commercially available media ("Ready-Mix" [RM] from Terry Fox, Vancouver, Canada and Stem Cell CFU Kit [SCCK]) from GIBCO, Grand Island, NY-Baxter Healthcare Corp., Deerfield, IL) to our standard laboratory media (SLM). Aliquots of mononuclear cells (MNC) from PB and BM donors were cultured in triplicate in the three media and CFU-GM and erythroid burst-forming units (BFU-E) were enumerated. Similar colony growth was achieved in all media for PB; modestly increased BM CFU-GM were noted in SCCK. SCCK and RM are easy to use, are commercially available with lot-controlled conditioned media (PHA-LCM), and may facilitate the standardization of CFU assays in blood banks and bone marrow processing laboratories.
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PMID:Colony-forming unit culture of bone marrow and peripheral blood stem cells: comparison of commercially available media. 136 35

The recently described Ta1 antigen is expressed by activated T cells in vitro and in vivo, as observed in patients with certain immune-mediated diseases, such as multiple sclerosis. In this paper we report on the tissue distribution of the Ta1 antigen. Serological testing of human tumour cell lines and immunohistochemical analysis of human tissue sections revealed a reactivity of the anti-Ta1 antibody with normal and malignant tissues of the upper gastro-intestinal tract, the biliary tract, exocrine pancreas and kidney. SDS-PAGE analysis of immunoprecipitates from 125I-labelled cells, employing the anti-Ta1 antibody, yielded a 113-115 kD band from three serologically Ta1 positive tumour cell lines, from a serologically Ta1 negative human EBV-transformed B lymphoblastoid cell line, from peripheral blood mononuclear cells (PBMC) and, as previously described, a 105 kD band from PHA activated T cells (Fox et al., 1984). After endoglycosidase F treatment similar bands of 85 kD were precipitated from activated T cells and from tumour cell lines. It is therefore likely that very similar glycoproteins, which differ only modestly in the size of carbohydrate chains, bear the Ta1 epitope on Ta1 positive tissues.
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PMID:Tissue distribution of the T cell activation antigen Ta1. Serological, immunohistochemical and biochemical investigations. 246 91

Foxp subfamily belongs to the Fox family of winged-helix transcription factors and plays critical roles in multiple biological processes including development and immunoregulation. However, little is known about the regulation and function of Foxp subfamily in fish immune system. In this study, we obtained the complete cDNAs of grass carp Foxp1a, Foxp1b and Foxp2. They possess the conserved leucine zipper domain, zinc finger domain and forkhead domain when compared with their mammalian counterparts, except that Foxp1a lacks the forkhead domain. Real-time RT-PCR analysis showed that their transcripts were mainly found in thymus, spleen and peripheral blood lymphocytes (PBLs). In grass carp PBLs, both LPS and PHA were effective in elevating Foxp1b mRNA levels but had no effect on Foxp1a mRNA, while only PHA affected Foxp2 mRNA expression. Using the same cell model, PHA was revealed to up-regulate mRNA expression of T-cell marker genes (CD4-like, CD8alpha and CD8beta) but not B-cell marker gene (IgM). Unlike PHA, LPS increased IgM mRNA level but did not affect T-cell marker gene expression. These findings suggest that PHA and LPS may act on distinct lymphocyte subpopulations in grass carp PBLs and provide evidence for the involvement of Foxp1b and Foxp2 in the activation of different lymphocyte subpopulations in grass carp.
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PMID:Characterization of grass carp (Ctenopharyngodon idellus) Foxp1a/1b/2: evidence for their involvement in the activation of peripheral blood lymphocyte subpopulations. 1992 98

Caenorhabditis elegans PHA-4 is a member of the FoxA group of transcription factors. PHA-4 is critical for development of the C. elegans pharynx and directly regulates most or all pharyngeal genes. The consensus binding site of PHA-4 has not been identified, with previous analysis of PHA-4 targets relying on the mammalian FoxA consensus. Here, we use in vitro and in vivo analyses to demonstrate three features of PHA-4 response elements. First, the PHA-4 consensus matches that of other FoxA proteins, but only a subset of possible sites is active in an in vivo assay. Second, sequence flanking the core PHA-4 site can influence the strength of reporter expression in vivo, as seen for other Fox proteins. Third, in the context of some pharyngeal promoters, PHA-4 response elements are flanked by distinct cis-regulatory elements that modulate response to PHA-4, generating gene expression in specific pharyngeal cell types.
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PMID:In vitro and in vivo characterization of Caenorhabditis elegans PHA-4/FoxA response elements. 2062 95