Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0016382 (flushing)
 6,387 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A radioimmunoassay has been developed for quantitation of ovine trophoblast protein-1 (oTP-1), a sheep conceptus secretory protein which allows for maintenance of the corpus luteum during early pregnancy. The assay was validated for dialysed and undialysed culture medium and pregnant uterine flushings ranging from no dilution (neat) to dilutions of 1:2500 for dialysed media, 1:100-1:1000 for undialysed media and 1:50-1:1000 for pregnant uterine flushings. The assay accurately measured oTP-1 added to undiluted and diluted dialysed and undialysed culture media and pregnant uterine flushings. No cross-reaction was detectable for bovine alpha or gamma interferon, bovine calmodulin, feline conceptus secretory proteins, equine conceptus secretory proteins, porcine conceptus secretory proteins, bovine conceptus secretory proteins and proteins in a uterine flushing collected from a non-pregnant ewe. Immunoreactivity in the assay matched that for oTP-1 throughout oTP-1 purification. This assay is the first validated assay which may be used to quantitate production of oTP-1 in culture or content of oTP-1 in uterine flushings.
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PMID:Development of a radioimmunoassay for ovine trophoblast protein-1, the antiluteolytic protein from the sheep conceptus. 337 49

Uterine luminal protein collected on d 17 (estrus = d 0) from Angus (n = 20) and Brahman (n = 19) cows of varying reproductive status was evaluated for immunosuppressive activity in vitro. Reproductive status consisted of the following categories: nonpregnant (uterine flushing was devoid of embryonic material); remnant (uterine flushing contained obvious degenerative fragments of embryonic tissue) and pregnant (uterine flushing contained an intact conceptus). Uterine protein was tested for suppressor activity in both phytohemagglutinin (PHA) and mixed lymphocyte culture (MLC) systems. Incorporation of [3H]-thymidine into 1 X 10(6) Angus or Brahman lymphocytes for PHA cultures and 5 X 10(5) lymphocytes from each breed for MLC experiments served as the index for lymphocyte blastogenesis. For all experiments, uterine luminal protein from nonpregnant, remnant and pregnant Angus and Brahman cows suppressed (P less than .01) lymphocyte blastogenesis for Angus and Brahman lymphocytes, respectively. In two lymphocyte-uterine protein specificity experiments containing PHA, uterine protein from Angus cows suppressed (P less than .01) blastogenesis of Brahman lymphocytes and uterine protein from Brahman cows suppressed (P less than .01) blastogenesis of Angus lymphocytes. Within and between breed comparisons of suppressor activity were evaluated when expressing uterine protein culture data as percentages of control (no test protein) cultures. At 200, 400 and 800 micrograms/ml of uterine protein, suppressor activity was consistently greater in secretions from pregnant than from nonpregnant cows for each breed. For between breed comparisons, there were nonsignificant trends toward greater suppressor activity for nonpregnant Angus than for nonpregnant Brahman cows at 200, 400 and 800 micrograms/ml of protein. Suppressor activity was greater (P less than .10) for pregnant Angus than for pregnant Brahman cows at 400 micrograms/ml of protein and tended to be greater for pregnant Angus than for Brahman cows at 800 micrograms/ml of protein. These data indicate that uterine secretory protein collected on d 17 from Angus and Brahman cows exerted immunosuppressive activity in vitro. Further, suppressor activity in each breed was greater in pregnant than in nonpregnant cows and suppressor activity tended to be greater for Angus than for Brahman cows.
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PMID:Immunosuppressive effect of uterine secretory protein from Angus and Brahman cows upon lymphocytes in vitro. 623 51

Ovarian characteristics, daily serum progesterone (P4) and estradiol-17 beta (E2) concentrations (d 7 through 17) and uterine luminal secretory protein components and histological variables were evaluated in parous Bos taurus (Angus, n = 20) and Bos indicus (Brahman, n = 19) cows. Cows were slaughtered on d 17 (estrus = d 0) for measurement of ovarian structures, flushing of uteri and removal of uterine tissue for histological evaluation. Cows were placed into one of three reproductive categories: nonpregnant, remnant (flushings contained remnants of embryonic tissue) or pregnant. For ovarian and uterine variables, there were only a few differences among reproductive categories within breeds. For combined categories, weight of the active ovary (containing the corpus luteum) was similar between breeds, but inactive ovarian (P less than .001) and follicular fluid (P less than .01) weights, stromal weight (P less than .01) and number of follicles less than 5 mm in diameter (P less than .01) for both ovaries combined were greater in Brahman than Angus cows. Corpus luteum weight (P less than .001), luteal P4 content (P less than .08) and number of follicles greater than 5 mm in diameter for both ovaries combined (P less than .05) were greater for Angus than for Brahman cows. Overall, mean serum P4 concentrations were greater in nonpregnant (P less than .05), pregnant (P less than .005) and combined (P less than .025) reproductive categories for Angus than corresponding categories of Brahman cows and mean serum E2 concentrations were greater in remnant (P less than .025) and combined (P less than .05) reproductive categories for Angus than corresponding categories of Brahman cows. Mean total uterine luminal protein was greater (P less than .05) in Angus than in Brahman cows for pregnant (23.4 vs 14.7 mg, respectively) and combined reproductive categories (22.4 vs 16.1 mg, respectively). Using electrophoretic analyses, percentage composition of three uterine specific cathode migrating protein bands and quantitative estimates of proteins with molecular weights (MW, X 10(-3)) of 9, 15.5, 34.2, 41.3, 46.2 and 183.1 were greater (P less than .05 to P less than .001) in uterine flushings from Angus than from Brahman cows. Uterine, myometrial and endometrial thicknesses, number of glands/microscopic field and uterine luminal epithelial cell height variables were generally greater (P less than .05 to P less than .001) in pregnant and combined reproductive categories for Angus than for Brahman cows.
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PMID:Ovarian and uterine morphology and function in Angus and Brahman cows. 651 77

Histamine intolerance results from a disequilibrium of accumulated histamine and the capacity for histamine degradation. Histamine is a biogenic amine that occurs to various degrees in many foods. In healthy persons, dietary histamine can be rapidly detoxified by amine oxidases, whereas persons with low amine oxidase activity are at risk of histamine toxicity. Diamine oxidase (DAO) is the main enzyme for the metabolism of ingested histamine. It has been proposed that DAO, when functioning as a secretory protein, may be responsible for scavenging extracellular histamine after mediator release. Conversely, histamine N-methyltransferase, the other important enzyme inactivating histamine, is a cytosolic protein that can convert histamine only in the intracellular space of cells. An impaired histamine degradation based on reduced DAO activity and the resulting histamine excess may cause numerous symptoms mimicking an allergic reaction. The ingestion of histamine-rich food or of alcohol or drugs that release histamine or block DAO may provoke diarrhea, headache, rhinoconjunctival symptoms, asthma, hypotension, arrhythmia, urticaria, pruritus, flushing, and other conditions in patients with histamine intolerance. Symptoms can be reduced by a histamine-free diet or be eliminated by antihistamines. However, because of the multifaceted nature of the symptoms, the existence of histamine intolerance has been underestimated, and further studies based on double-blind, placebo-controlled provocations are needed. In patients in whom the abovementioned symptoms are triggered by the corresponding substances and who have a negative diagnosis of allergy or internal disorders, histamine intolerance should be considered as an underlying pathomechanism.
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PMID:Histamine and histamine intolerance. 1749 Sep 52