Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0016382 (flushing)
 6,387 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Administration of estradiol-17 beta (E) to ovariectomized (ovx) sheep results in the synthesis and release of an M(r) 90,000-92,000 glycoprotein into the oviductal lumen and into culture medium of ampullar explants (Biol Reprod 1992; 47:889-902). The objective of this study was to determine when and from what region of the oviduct the M(r) 90,000-92,000 glycoprotein is synthesized and released during early pregnancy. Estrous ewes were bred to intact rams of known fertility, and oviducts were obtained at estrus (Day 0) and at Days 1.5, 2, 3, 4, 6, and 16 of pregnancy. Pregnancy was verified by the presence of a fertilized egg or developing conceptus and a functional corpus luteum. Oviductal secretions were collected by flushing oviducts with saline and by explant culture. The oviductal fimbria, ampulla, and isthmus were individually cultured (24 h) in the presence of 3H-leucine (3H-leu) or 3H-glucosamine (3H-glcN). The presence of the M(r) 90,000-92,000 glycoprotein in oviductal flushings and culture medium was determined by fluorography and Western blotting. The M(r) 90,000-92,000 protein was present in SDS gels and blots of oviductal flushings from animals through Days 4-6 of pregnancy, but not in flushings from Day 16 pregnant animals or from ovx, untreated animals. This protein was present in 3H-leu- and 3H-glcN-labeled culture medium of the oviductal ampulla (Days 0, 1.5, 2, 3, 4, 6, and 16) and fimbria (Days 0, 1.5, 2, 3, and 4) during early pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:An estrogen-dependent glycoprotein is synthesized and released from the oviduct in a temporal- and region-specific manner during early pregnancy in the ewe. 845 22

One-step hydrolysis of chitin to release glucosamine for quantitation was achieved by combining a chitin-containing sample (10-200 mg of sample size) in a test tube with 1 mL of 10 M HCl followed by vacuum treatment for 10 min, incubation at 28 degrees C for 30 min, replenishment with 3 mL of deionized water, nitrogen flushing, screw capping, and heat treatment at 140 degrees C for 60 min. A phosphate buffer solution (pH 12.5, 0.2 M) was effective in pH stabilization and enhancing colorimetric determination of glucosamine content. When the modified procedure was applied to analyze glucosamine content in the mycelia of various molds, glucosamine content varied mainly depending on mold species. In estimations of mold growth of the uninoculated peanut kernels incubated under a humidified condition for 5 weeks, cooked rice and soybean inoculated with conidia of Aspergillus oryzae for koji preparation, logarithms of the internal mold populations and glucosamine contents both increased with increases of incubation time. The modified procedure provided a rapid and reliable estimation of mold growth in various substrates.
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PMID:A modified chemical procedure for rapid determination of glucosamine and its application for estimation of mold growth in peanut kernels and koji. 1055 85