Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0016382 (flushing)
 6,387 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A new simple and fast noncovalent coating method based on poly(1-vinylpyrrolidone-co-2-dimethylaminoethyl methacrylate) copolymer was developed for CE. Merely 2 min flushing of the capillary with the poly(1-vinylpyrrolidone-co-2-dimethylaminoethyl methacrylate) copolymer was required. The copolymer is adsorbed onto the fused-silica surface by hydrogen bonding and electrostatic interactions. EOF was almost totally suppressed over a wide pH range. The coating conditions (flushing time, copolymer concentration, and the concentration and pH of background electrolyte solution) and the stability of the coating were optimized, and the coated capillary was successfully applied to the fast separation of four basic proteins: lysozyme, cytochrome c, ribonuclease A, and alpha-chymotrypsinogen A. Separation efficiencies were high, ranging from 386 000 to 738 000 plates/m at 40 mM pH 4.0 acetate buffer being comparable to values obtained on classical covalent PVP-coated capillary. The RSD of migration times of basic proteins for 200 times successive runs were all below 1.0% (n=200, 3 days). A successful capillary performance was demonstrated also to the separation of low- and high-density lipoproteins at acidic pH.
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PMID:Noncovalent poly(1-vinylpyrrolidone)-based copolymer coating for the separation of basic proteins and lipoproteins by CE. 1988 86

Antibacterial photodynamic treatment (APDT) might prove valuable as an alternative to the application of chemoantibiotics for the treatment of staphylococcal infections. The rapid uptake of the photosensitizing agent into bacteria allows for selective killing of microorganisms whilst sparing the eukaryotic host tissue, but also requires removal of excessive dye after the incubation period. We tested water-soluble formulations of hypericin (PVP-hypericin) and m-tetrahydroxyphenylchlorin (Fospeg), which could be applied as aqueous sprays and removed easily by flushing with buffers, for their efficiency in killing Staphylococcus aureus. For both sensitizers, 100 nM of the photoactive substance incubated for 5 min and illuminated for 30 min at 75 mW cm(-2) lead to a 4-5 log unit reduction in bacterial count. At a concentration of 300 nM (incubation time 5 min), 30 min illumination at 25 mW cm(-2) is more effective than 10 min illumination at 75 mW cm(-2) (both resulting in the same fluence). We suggest both substances as promising candidates for treatment of staphylococcal infections in wounds with APDT.
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PMID:Antibacterial photodynamic therapy using water-soluble formulations of hypericin or mTHPC is effective in inactivation of Staphylococcus aureus. 2022 63