Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0016382 (flushing)
 6,387 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Flow-through aquifer columns were operated for 12 weeks to evaluate the benefits of aerobic biostimulation for the bioremediation of source-zone soil contaminated with chlorobenzenes (CBs). Quantitative Polymerase Chain Reaction (qPCR) was used to measure the concentration of total bacteria (16S rRNA gene) and oxygenase genes involved in the biodegradation of aromatic compounds (i.e., toluene dioxygenase, ring hydroxylating monooxygenase, naphthalene dioxygenase, phenol hydroxylase, and biphenyl dioxygenase). Monochlorobenzene, which is much more soluble than dichlorobenzenes, was primarily removed by flushing, and biostimulation showed little benefit. In contrast, dichlorobenzene removal was primarily due to biodegradation, and the removal efficiency was much higher in oxygen-amended columns compared to a control column. To our knowledge, this is the first report that oxygen addition can enhance CB source-zone soil bioremediation. Analysis by qPCR showed that whereas the biphenyl and toluene dioxygenase biomarkers were most abundant, increases in the concentration of the phenol hydroxylase gene reflected best the higher dichlorobenzene removal due to aerobic biostimulation. This suggests that quantitative molecular microbial ecology techniques could be useful to assess CB source-zone bioremediation performance.
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PMID:Aerobic bioremediation of chlorobenzene source-zone soil in flow-through columns: performance assessment using quantitative PCR. 1796 Apr 85

Polybrominated diphenyl ethers (PBDEs) resistant to degradation have significant environmental impacts. Anaerobic reductive debromination and aerobic oxidation of PBDEs by microorganisms are main removal mechanisms during natural attenuation, but previous studies often focused on the process under either aerobic or anaerobic condition leading to unsatisfactory removal. The present study aims to remove PBDEs by employing alternating anaerobic-aerobic condition, which is common in inter-tidal mangrove sediments, and elucidate the degradation pathways. During 40-week experiment, BDE-47 reduced with an accumulation of tri-BDEs and di-BDEs as debromination products in all sediments. However, the removal percentages of BDE-47 and the concentrations of debromination congeners varied among flushing regimes. Sediments under less frequent flushing regime (longer duration of aerobic period) had significantly lower concentration and proportion of debromination products, especially BDE-17, than that under more frequent regime (longer anaerobic period). BDE-17 then went through aerobic degradation pathway, as evidenced by the accumulation of its hydroxylation form. Microbial analyses further revealed that less frequent regime favored accumulation of biphenyl dioxygenase gene for aerobic degradation, while more frequent tidal regime promoted growth of dehalogenating bacteria for reductive debromination. This study first time demonstrated that PBDEs in contaminated sediments could be removed under alternating anaerobic-aerobic conditions.
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PMID:Degradation of BDE-47 in mangrove sediments under alternating anaerobic-aerobic conditions. 3120 18