Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0015695 (
fatty liver
)
13,941
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Hydrogen peroxide (H
2
O
2
) is a central reactive oxygen species (ROS) that contributes to diseases from obesity to cancer to neurodegeneration but is also emerging as an important signaling molecule. We now report a versatile histochemical approach for detection of H
2
O
2
that can be employed across a broad range of cell and tissue specimens in both healthy and disease states. We have developed a first-generation H
2
O
2
-responsive analogue named Peroxymycin-1, which is based on the classic cell-staining molecule puromycin and enables covalent staining of biological samples and retains its signal after fixation. H
2
O
2
-mediated boronate cleavage uncages the puromycin aminonucleoside, which leaves a permanent and dose-dependent mark on treated biological specimens that can be detected with high sensitivity and precision through a standard immunofluorescence assay. Peroxymycin-1 is selective and sensitive enough to image both exogenous and endogenous changes in cellular H
2
O
2
levels and can be exploited to profile resting H
2
O
2
levels across a panel of cell lines to distinguish metastatic,
invasive cancer
cells from less
invasive cancer
and nontumorigenic counterparts, based on correlations with ROS status. Moreover, we establish that Peroxymycin-1 is an effective histochemical probe for in vivo H
2
O
2
analysis, as shown through identification of aberrant elevations in H
2
O
2
levels in liver tissues in a murine model of nonalcoholic
fatty liver
disease, thus demonstrating the potential of this approach for studying disease states and progression associated with H
2
O
2
. This work provides design principles that should enable development of a broader range of histochemical probes for biological use that operate via activity-based sensing.
...
PMID:Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining. 2972 74
