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Target Concepts:
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Query: UMLS:C0015695 (
fatty liver
)
13,941
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Some members of
Rhododendron
genus are traditionally used as medicinal plants for arthritis, acute and chronic bronchitis, asthma, pain, inflammation,
rheumatism
, hypertension and metabolic diseases. To the best of our knowledge, there is no report on the protective effects of
R. oldhamii
leaf extract on non-alcoholic fatty liver disease (NAFLD)
in vivo
and
in vitro
. In this study, the effects of
R. oldhamii
leaf extract on inhibiting the free fatty acid (FFA)-induced accumulation of fat in HepG2 cells and on improving
fatty liver
syndrome in mice with high fat diet (HFD)-induced NAFLD were investigated. For the
in vitro
assay, HepG2 cells were treated with FFAs (oleate/palmitate = 2:1) with or without treatment with
R. oldhamii
leaf ethyl acetate (EtOAc) fraction to observe lipid accumulation using Nile red and oil red O stains. For the
in vivo
assay, C57BL/6 mice were randomly assigned to three groups (
n
= 5), including the normal diet group, the HFD group and the HFD+EtOAc group. After 11 weeks, body weight, serum biochemical indices and the mRNA expressions of the liver tissue, as well as the outward appearance, weight and histopathological analysis of liver and adipose tissues were evaluated. Among the fractions derived from
R. oldhamii
leaf, the EtOAc fraction exhibited a strong fat-accumulation inhibitory activity. Following reverse-phase high-performance liquid chromatography (HPLC), four specific phytochemicals, including (2
R
, 3
R
)-astilbin (AS), hyposide (HY), guaijaverin (GU) and quercitrin (QU), were isolated and identified from the EtOAc fraction of
R. oldhamii
leaf extract. Among them, AS and HY showed excellent fat-accumulation inhibitory activity. Thus, the EtOAc fraction of
R. oldhamii
leaf and its derived phytochemicals have great potential in preventing FFA-induced fat accumulation. In addition, the EtOAc fraction of
R. oldhamii
leaf significantly improved
fatty liver
syndrome and reduced total cholesterol (TC) and triglyceride (TG) in HFD-induced NAFLD mice at a dosage of 200 mg/kg BW. These results demonstrated that the methanolic extracts from
R. oldhamii
leaf have excellent inhibitory activities against fat accumulation and anti-NAFLD activities and thus have great potential as a natural health product.
...
PMID:
Rhododendron oldhamii
leaf extract improves fatty liver syndrome by increasing lipid oxidation and decreasing the lipogenesis pathway in mice. 2882 23
