Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0015674 (chronic fatigue syndrome)
 2,978 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The RSV IV polypeptide, molecular weight ratio (Mr = 10,000), which is produced by the rat seminal vesicle, has previously been suggested to be associated with another polypeptide in the gland secretion (Higgins et al., '76). This study provides that RSV IV is a component of a protein shown by immunoassays, electrophoresis, and amino acid composition analysis to contain, together with RSV IV, the seminal vesicle secretory RSV V polypeptide (Mr = 13,000). This RSV IV-RSV V complex (namely CFS protein) had an isoelectric point at pH 7.2 and an approximate molecular weight of 22,000 daltons. This complex inhibits the previously reported in vitro binding of the isolated RSV IV to epididymal sperm cells, thus suggesting a functional role for the RSV IV-RSV V interaction.
 ...
PMID:Interaction of proteins RSV IV and RSV V in rat seminal vesicle secretion. 272 6

We report in this paper that proteins from the surface of ejaculated spermatozoa contain antigenic determinants cross-reacting with a rabbit antiserum raised against native CFS, a protein secreted from the rat seminal vesicle and composed of two subunits, namely RSV IV and RSV V. Conversely, no such proteins could be extracted from cauda epididymal spermatozoa. The cross-reacting proteins derived from the ejaculated spermatozoa were analyzed by SDS-PAGE. An electrophoretic pattern different than that expected for native CFS in denaturing conditions was found. In vitro reconstitution experiments showed that labeled native CFS is able to bind cauda epididymal spermatozoa. The CFS protein recovered from the sperm surface was examined and alterations of its structure were also noted. The sperm-coating abilities of CFS and of its RSV IV subunit are discussed.
 ...
PMID:Detection of sperm-coating antigens immunologically related to a seminal protein in rat. 324 84

The heterodimeric sperm-coating protein CFS was previously localised on the middle-piece region of rat spermatozoa by anti-CFS rabbit antibodies. CFS-immunorelated antigens were detected in the secretion of the water buffalo seminal vesicle by protein electrophoresis and Western blotting. Spermatozoa from buffalo epididymal cauda were incubated with the rat antigen and, upon immunostaining with anti-CFS antibodies and goat anti-rabbit fluorescein isothiocyanate (FITC)-conjugated IgGs, CFS was found attached on both the post-acrosomal region and the tail. Indirect immunofluorescence analysis permitted the localisation of CFS-related antigens on the same domains of buffalo ejaculated spermatozoa. These results suggest that the buffalo antigens not only share some epitopes with the homologous rat antigen but may also have some of its functional properties. Ejaculated spermatozoa were capacitated in vitro and then assayed for their content of CFS-like antigens. An inverse relationship was found between the levels of capacitation and the amounts of antigens detected, thus suggesting that the in vitro treatment was effective at removing CFS-related proteins from the cell surface. Titration of these proteins to monitor plasma membrane changes during sperm manipulation or to evaluate sperm quality is proposed.
 ...
PMID:Localisation and capacitation-dependent loss of buffalo sperm-coating antigens shared with rat sperm. 788 16