Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0014118 (
endocarditis
)
15,629
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Kingella kingae
is a significant pediatric pathogen responsible for bone and joint infections, occult bacteremia, and
endocarditis
in early childhood. Past efforts to detect this bacterium using culture and broad-range 16S rRNA gene PCR assays from clinical specimens have proven unsatisfactory; therefore, by the late 2000s, these were gradually phased out to explore the benefits of specific real-time PCR tests targeting the
groEL
gene and the RTX locus of
K. kingae
However, recent studies showed that real-time PCR (RT-PCR) assays targeting the
Kingella
sp. RTX locus that are currently available for the diagnosis of
K. kingae
infection lack specificity because they could not distinguish between
K. kingae
and the recently described
Kingella negevensis
species. Furthermore,
in silico
analysis of the
groEL
gene from a large collection of 45
K. kingae
strains showed that primers and probes from
K. kingae
groEL
-based RT-PCR assays display a few mismatches with
K. kingae groEL
variations that may result in decreased detection sensitivity, especially in paucibacillary clinical specimens. In order to provide an alternative to
groEL
- and RTX-targeting RT-PCR assays that may suffer from suboptimal specificity and sensitivity, a
K. kingae
-specific RT-PCR assay targeting the
malate dehydrogenase
(
mdh
) gene was developed for predicting no mismatch between primers and probe and 18 variants of the
K. kingae mdh
gene from 20 distinct sequence types of
K. kingae
This novel
K. kingae
-specific RT-PCR assay demonstrated high specificity and sensitivity and was successfully used to diagnose
K. kingae
infections and carriage in 104 clinical specimens from children between 7 months and 7 years old.
...
PMID:A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens. 2987 89
