Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0014070 (
encephalomyelitis
)
13,017
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian
encephalomyelitis
virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural
protein P1
region of the AEV genome. An avian
encephalomyelitis
virus-specific 619-base pair cDNA product was amplified by these primers from five reference/field strains of AEVs but not from 10 other avian pathogenic viruses and bacteria. The RT-PCR assay developed in this study was found to be sensitive and specific with as little as 10 pg of avian
encephalomyelitis
virus RNA detected using gel electrophoresis. Furthermore, AEV-RT-PCR was able to detect AE virus from chicken embryo brain at 3 days postinoculation as compared with the AE agar gel precipitation test (AGP), which required up to 11 days of incubation in the embryos.
...
PMID:Reverse transcriptase-polymerase chain reaction to detect avian encephalomyelitis virus. 1609 27
