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Query: UMLS:C0012872 (DNA marker)
 929 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Recently, the Fusarium genus has been narrowed based upon phylogenetic analyses and a Fusarium-like clade was adopted. The few species of the Fusarium-like clade were moved to new, re-installed or existing genera or provisionally retained as "Fusarium." Only a limited number of reference strains and DNA marker sequences are available for this clade and not much is known about its actual species diversity. Here, we report six strains, preserved by the Belgian fungal culture collection BCCM/IHEM as a Fusarium species, that belong to the Fusarium-like clade. They showed a slow growth and produced pionnotes, typical morphological characteristics of many Fusarium-like species. Multilocus sequencing with comparative sequence analyses in GenBank and phylogenetic analyses, using reference sequences of type material, confirmed that they were indeed member of the Fusarium-like clade. One strain was identified as "Fusarium" ciliatum whereas another strain was identified as Fusicolla merismoides. The four remaining strains were shown to represent a unique phylogenetic lineage in the Fusarium-like clade and were also found morphologically distinct from other members of the Fusarium-like clade. Based upon phylogenetic considerations, a new genus, Pseudofusicolla gen. nov., and a new species, Pseudofusicolla belgica sp. nov., were installed for this lineage. A formal description is provided in this study. Additional sampling will be required to gather isolates other than the historical strains presented in the present study as well as to further reveal the actual species diversity in the Fusarium-like clade.
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PMID:Unique Phylogenetic Lineage Found in the Fusarium-like Clade after Re-examining BCCM/IHEM Fungal Culture Collection Material. 2779 62

It is difficult to distinguish the genera Svistella and Paratrigonidium on the basis of morphology. Gorochov postulated that Svistella spp. possess the following identification characters: posterior lateral lobes of epiphallus long and narrow at apex, guide rod elongate, attachment plate of the spermatophore consisting of three sclerites (a narrow middle sclerite bearing no protruding structure, and a pair of lateral ones extending to the sides). These features have not been extensively evaluated. In this study, the COI gene was sequenced and was used as a DNA marker to distinguish two genera. The results showed that each genus formed a monophyly, respectively. One new species, Svistella fuscoterminata sp. nov., was found in Yunnan, China. Although its genitalia were similar to that of Paratrigonidium spp., the molecular result supported the new species belonging to Svistella. The specimens are deposited in East China Normal University, Biology of History Museum (ECNU) and Shanghai Entomological Museum (SEM).
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PMID:Settlement of the classification of Svistella Gorochov and Paratrigonidium Brunner von Wattenwyl using morphology and molecular techniques, with the description of a new species from Yunnan, China (Orthoptera: Trigonidiidae: Trigonidiinae). 2969 Feb 84

Cytospora is a genus including important phytopathogens causing severe dieback and canker diseases distributed worldwide with a wide host range. However, identification of Cytospora species is difficult since the currently available DNA sequence data are insufficient. Aside the limited availability of ex-type sequence data, most of the genetic work is only based on the ITS region DNA marker which lacks the resolution to delineate to the species level in Cytospora. In this study, three fresh strains were isolated from the symptomatic branches of Elaeagnus angustifolia in Xinjiang Uygur Autonomous Region, China. Morphological observation and multi-locus phylogenetic analyses (ITS, LSU, ACT and RPB2) support these specimens are best accommodated as a distinct novel species of Cytospora. Cytospora elaeagnicola sp. nov. is introduced, having discoid, nearly flat, pycnidial conidiomata with hyaline, allantoid conidia, and differs from its relatives genetically and by host association.
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PMID:Cytospora elaeagnicola sp. nov. Associated with Narrow-leaved Oleaster Canker Disease in China. 3162 Mar 8