Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0012739 (disseminated intravascular coagulation)
 8,673 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To facilitate functional studies of novel myosins, we have developed a strategy for characterizing the mechanochemical properties of motors isolated by immunoadsorption directly from small amounts of crude tissue extracts. In this initial study, silica beads coated with an antibody that specifically recognizes the tail of myosin-V were used to immunoadsorb this motor protein from brain extracts. The myosin-containing beads were then positioned with optical tweezers onto actin filaments nucleated from Limulus sperm acrosomal processes and observed for motility using high resolution video DIC microscopy. The addition of brush border spectrin to the motility chamber enabled the growth of stable actin filament tracks that were approximately 4-fold longer than filaments grown in the absence of this actin crosslinking protein. The velocity of myosin-V immunoadsorbed from brain extracts was similar to that observed for purified myosin-V that was antibody-linked to beads or assessed using the sliding actin filament assay. Motile beads containing myosin-V immunoadsorbed from brain extracts bound poorly to nucleated actin filaments and were incapable of linear migrations following the addition of a different antibody that specifically recognizes the motor-containing head domain of myosin-V. Myosin-V motility was most robust in the absence of Ca2+. Interestingly, skeletal muscle tropomyosin and brush border spectrin had no detectable effect on myosin-V mechanochemistry. Myosin-V containing beads were also occasionally observed migrating directly on acrosomal processes in the absence of exogenously added actin.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:In vitro motility of immunoadsorbed brain myosin-V using a Limulus acrosomal process and optical tweezer-based assay. 761 69

On the basis of histological and immunohistochemical studies of 50 uterine scrapes after abortions and labor due to late bleedings, the authors identified three major types of the so-called placental polyps: (a) preserved villi (56%); (b) clusters of destructive villi (38%); (c) isolated viable cotyledons (6%). Two pathogenetic mechanisms of uterine hemorrhages have been substantiated: firstly, preservation of the brush border of a syncytiotrophoblast (including the presence of placental phosphatase) maintains the anticoagulative properties of villi; this appears in cases of postabortion hemorrhages and at the most in preserved cotyledons. Secondly, the thromboplastic properties of the preserved villi play a leading role in the pathogenesis of uterine hemorrhage in the scrapes where necrotic villi with epithelial remains are prevalent, i.e. the situation is similar to the hypocoagulative phase of isolated chronic disseminated intravascular coagulation.
 ...
PMID:[The pathogenesis of uterine hemorrhages in the so-called placental polyps]. 1880 25