Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0011860 (
type 2 diabetes
)
57,723
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recently, CeNPs have emerged as an effective therapeutic agent due to their redox-active nature encompassing the ability to switch between +4 or +3 oxidation states of surface "Ce" atoms. CeNPs with predominantly high Ce +4 oxidation state have been shown to exhibit biological catalase enzyme-like activity. Catalase enzyme is naturally present in mammalian cells and facilitates the protection from reactive oxygen species (ROS), generated due to decomposition of hydrogen peroxide (H
2
O
2
). Inactivation of cellular catalase enzyme is known to cause several diseases such as acatalasemia,
type 2 diabetes
mellitus, and vitiligo. In this study, we have artificially inhibited the activity of cellular catalase enzyme from human liver cells (WRL-68) using 3-Amino-
1,2,4-Triazole
(3-AT). Further, CeNPs was used for imparting protective effect against the deleterious effects of elevated cellular H
2
O
2
concentration. Our results suggest that CeNPs (+4) can protect hepatic cells from cytotoxicity and genetic damage from the high concentrations of H
2
O
2
in the absence of functional catalase enzyme. CeNPs were efficiently internalized in WRL-68 cells and effectively scavenge the free radicals generated due to elevated H
2
O
2
inside the cells. Additionally, CeNPs were also shown to protect cells from undergoing early apoptosis and DNA damage induced due to the 3-AT exposure. Moreover, CeNPs did not elicit the natural antioxidant defense system of the cells even in the absence of functional catalase enzyme, suggesting that the observed protection was due to the H
2
O
2
degradation activity of CeNPs (+4). Our finding substantiates the reinforcement of CeNPs as pharmacological agents for the treatment of diseases related to nonfunctional biological catalase enzyme in the mammalian cells.
...
PMID:Redox-dependent catalase mimetic cerium oxide-based nanozyme protect human hepatic cells from 3-AT induced acatalasemia. 3058 18
