Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0011570 (depression)
 172,036 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The objective of this study was to investigate the effects of nutrient density and dietary energy source on performance and immune function of weanling pigs that were either challenged or not challenged with Escherichia coli lipopolysaccharide (LPS). A basal diet was formulated to contain 14 g CP/MJ DE and 7 g lysine/100 g CP. Sulfur amino acids, threonine and tryptophan were kept constant relative to lysine. Experimental diets were mixed using 70 parts basal diet and either 30 parts starch or an isocaloric amount (14 parts) of lard. Diets were fed either for ad libitum intake or on a pair-feeding basis to evaluate effects of diet nutrient density or source of energy, respectively. On d 9 and 25, pigs were challenged i.m. with either 1 mL of a LPS solution or a control solution. Lymphocyte blastogenesis was measured 2 d after the LPS administration and antibody response to sheep red blood cells (SRBC) or ovalbumin was determined 3 d after challenge. No interactive effects on performance were observed between LPS challenge and energy density or source of energy (P > .10). Injection of LPS tended to reduce feed intake and daily gain (P < .10), but not efficiency of feed or energy utilization. Addition of fat to the diets improved feed efficiency and efficiency of energy utilization for gain (P < .05). No consistent effects of LPS challenge, energy density, or source of energy were observed for lymphocyte blastogenesis. Antibody response to ovalbumin, but not to SRBC, was decreased by fat (P < .05). Results indicate that increasing energy density of the diet did not alter the performance depression due to LPS challenge. Addition of fat to the diet improved feed efficiency and efficiency of energy conversion but may depress the humoral immune response. Effects of fat on the immune response may depend on the immune status of the pig.
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PMID:Effects of immune challenge, dietary energy density, and source of energy on performance and immunity in weanling pigs. 890 12

Calcineurin is a serine/threonine protein phosphatase 2B widely distributed in the brain. However, its role in brain function remains unknown. Recent data indicate that calcineurin can participate in long-term depression or long-term potentiation in rat hippocampus. Obviously, calcineurin can also be involved in numerous brain diseases, such as ischaemic hippocampal damage when the protein dephosphorylation system is markedly altered and hyperphosphorylation of the microtubule system in Alzheimer's disease. Besides, abnormal phosphorylation of the cytoskeletal proteins affecting the synaptic signalling can lead to different pathological disorders in the brain. In this study we analysed in more detail the localization of calcineurin in neuronal elements by using confocal microscopy and immunocytochemical approaches to record the enzyme expression in cultured rat dorsal root ganglion neurons. This is the first report showing that calcineurin immunoreactivity is highly expressed in dorsal root ganglion neurons and it is localized mainly near the inner surface of the plasma membrane. Immunostaining of these cells by anti-beta subunits of voltage-operated Ca2+ channels showed that distribution of calcium channel beta-subunit and calcineurin is very similar. Our findings confirm that the function of calcineurin can be directly connected with the activity of voltage-operated calcium channels.
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PMID:Evidence for colocalization of calcineurin and calcium channels in dorsal root ganglion neurons. 915 45

Physical fatigability and avoidance of physically demanding tasks in chronic fatigue syndrome (CFS) were assessed by the achievement or nonachievement of 85% of age-predicted maximal heart rate (target heart rate, THR) during incremental exercise. The association with functional status impairment, somatization, and psychopathology was examined. A statistically significant association was demonstrated between this physical fatigability variable and impairment, and a trend was found for an association with somatization. No association was demonstrated with psychopathology. These results are in accordance with the cognitive-behavioral model of CFS, suggesting a major contribution of avoidance behavior to functional status impairment; however, neither anxiety nor depression seem to be involved in the avoidance behavior. Aerobic work capacity was compared between CFS and healthy controls achieving THR. Physical deconditioning with early involvement of anaerobic metabolism was demonstrated in this CFS subgroup. Half of the CFS patients who did not achieve THR did not reach the anaerobic threshold. This finding argues against an association in CFS between avoidance of physically demanding tasks and early anaerobic metabolism during effort.
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PMID:Physical fatigability and exercise capacity in chronic fatigue syndrome: association with disability, somatization and psychopathology. 916 Feb 76

Antifreeze proteins (AFP) inhibit ice growth by surface adsorption that results in a depression of the freezing point below the melting point. The maximum level of this thermal hysteresis shown by the four structurally unrelated fish AFP is approximately 1.5 degrees C. In contrast, hemolymph and crude extracts from insects can have 5 degrees to 10 degrees C of thermal hysteresis. Based on the isolation, cloning, and expression of a thermal hysteresis protein (THP) from spruce budworm (Choristoneura fumiferana), the vastly greater activity is attributable to a 9 kDa protein. This novel, threonine- and cysteine-rich THP has striking effects on ice crystal morphology, both before and during freezing. It is also 10 to 30 times more active than any known fish AFP, offering the prospect of superior antifreeze properties in cryoprotective applications.
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PMID:The antifreeze potential of the spruce budworm thermal hysteresis protein. 930 95

The effect of microbial phytase supplementation on CP and amino acid (AA) digestibility was investigated in a 28-d trial using 360 sexed, day-old broiler chickens fed corn-soybean meal diets. The experimental design was a completely randomized one with a 3 x 2 factorial arrangement of treatments. The variables included P and Ca levels and phytase: P and Ca levels were: normal P-normal Ca [0.45% available P (Pa), 1.0% Ca], low P-normal Ca (0.35% Pa, 1.0% Ca), and low P-low Ca (0.35 Pa and 0.6% Ca); and phytase at 0 and 600 U/kg diet. Phytase supplementation increased body weight gain (P < 0.014) and feed intake (P < 0.004) at 19 d in male chickens; in females, phytase increased (P < 0.012) only body weight gain at 19 d. The low P-normal Ca diet reduced (P < 0.05) feed intake and body weight gain in both sexes at 7, 14, and 19 d, compared to the normal P-normal Ca diet; the reduction of Ca in the low P diet prevented the above depression, resulting in body weight gain and feed intake to a level comparable to that of the normal P-normal Ca diet. Microbial phytase supplementation had no effect (P < 0.065) on the apparent ileal digestibility (AID) of CP or any AA except Met and Phe in male broiler chickens. In females, adding phytase increased the AID of all AA except Lys, Met, Phe, and Pro. The low P-normal Ca diet reduced (P < 0.05) the AID of Phe, Asp, and Ser in male chickens and reduced the AID of all the AA except Met and Pro in females compared to the normal P-normal Ca diet. The reduction of Ca in the low P diet prevented the depression of the AID of the AA caused by the low P-normal Ca diet, resulting in AID of AA having a level comparable to that of the normal P-normal Ca diet in both sexes. Phytase supplementation did not have any effect (P > 0.05) on apparent "fecal" digestibility (AFD) of CP or any of the AA in male chickens; however, in female chickens it increased the AFD of Thr, Asp, Glu, and Ser. In summary, phytase supplementation increased growth performance in both sexes; increased AID and AFD of most of the AA, particularly in female chickens. The optimum growth performance and AA digestibilities were obtained with the lowest input of resources, in the low P-low Ca diet supplemented with microbial phytase.
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PMID:Apparent digestibility of protein and amino acids in broiler chickens fed a corn-soybean diet supplemented with microbial phytase. 943 93

Conversion of the three mapped threonine phosphorylation sites in the myosin II heavy chain tail to alanines results in a mutant (3XALA) in Dictyostelium discoideum, which displays constitutive myosin overassembly in the cytoskeleton and increased cortical tension. To assess the importance of myosin phosphorylation in cellular translocation and chemotaxis, 3XALA mutant cells have been analyzed by 2D and 3D computer-assisted methods in buffer, in a spatial gradient of cAMP, and after the rapid addition of cAMP. 3XALA cells crawling in buffer exhibit distinct abnormalities in cellular shape, the maintenance of polarity and the complexity of the pseudopod perimeter. 3XALA cells crawling in buffer also exhibit a decrease in directionality. In a spatial gradient of cAMP, the behavioral defects are accentuated. In a spatial gradient, 3XALA cells exhibit a repeating 1- to 2-min behavior cycle in which the shape of each cell changes abnormally from elongate to extremely wide with lateral, opposing pseudopods. At the end of each cycle, 3XALA cells turn 90 degrees into the left or right lateral pseudopod, resulting in a dramatic depression in chemotactic efficiency, even though 3XALA cells are chemotactically responsive to cAMP. These results demonstrate that the phosphorylation of myosin II heavy chain plays a critical role in the maintenance of cell shape and in persistent translocation in a spatial gradient of chemoattractant.
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PMID:Phosphorylation of the Dictyostelium myosin II heavy chain is necessary for maintaining cellular polarity and suppressing turning during chemotaxis. 945 12

Previous research has revealed that major depression is accompanied by disorders in excitatory amino acids, e.g. glutamate and aspartate, and alterations in serum levels of other amino acids, e.g. serine, glycine and taurine. The aim of the present study was to examine serum levels of aspartate, asparagine, glutamate, glutamine, serine, glycine, threonine, histidine, alanine, taurine and arginine in major depression patients with treatment-resistant depression (TRD). No significant differences in the serum concentrations of any of the above amino acids could be found between patients with and without TRD and normal controls. Non-responders to treatment with antidepressants during a period of 5 weeks were characterized by significantly lower serum levels of aspartate, asparagine, serine, threonine and taurine. A 5-week period of treatment with antidepressants significantly reduced the serum levels of aspartate, glutamate and taurine, and significantly increased the serum concentrations of glutamine. The results suggest that alterations in serum levels of aspartate, asparagine, serine, threonine and taurine may predict the subsequent response to treatment with antidepressants, and that the latter may modulate serum levels of excitatory amino acids and taurine.
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PMID:Serum levels of excitatory amino acids, serine, glycine, histidine, threonine, taurine, alanine and arginine in treatment-resistant depression: modulation by treatment with antidepressants and prediction of clinical responsivity. 957 Apr 92

The neural substrates of learning and memory most likely involve activity-dependent long-term changes in synaptic strength, including long-term potentiation and long-term depression. A critical element in the cascade of events hypothesized to underlie such changes in synaptic function is modification of protein phosphorylation. Long-term depression is thought to involve decreases in protein phosphorylation, which could result from reduction in protein kinase activity and/or enhancement in protein phosphatase activity. We present here direct evidence that long-term depression in the hippocampus in vivo is associated with an increase in the activity of the serine/threonine phosphatases 1 and 2A. The increase in activity of phosphatase 1 was transient, whereas that of phosphatase 2A lasted > 65 min after the induction of long-term depression. Blockade of long-term depression prevented the observed increases in phosphatase activity, as did selective inhibition of phosphatase 1 and 2A. Induction of long-term depression had no effect on the level of either phosphatase, which suggests that our results reflect increases in the intrinsic activity of these two enzymes. Our findings are consistent with a model of synaptic plasticity that implicates protein dephosphorylation by serine/threonine phosphatases in the early maintenance and/or expression of long-term depression of synaptic strength.
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PMID:Transient and persistent increases in protein phosphatase activity during long-term depression in the adult hippocampus in vivo. 969 9

1. The effects of an opioid (D-Ser-Leu-enkephalin-Thr; DSLET) were tested on synaptic actions of non-nociceptive afferents: group I and II muscle afferents and low-threshold skin afferents. They were tested on population EPSPs (field potentials) evoked in the dorsal horn and the intermediate zone of mid-lumbar segments, and on monosynaptically evoked responses of single interneurones at the same location. DSLET was applied locally (ionophoretically) at locations at which the field potentials were maximal and close to the selected neurones. 2. DSLET potently depressed transmission from group II muscle afferents and from low-threshold skin afferents. Transmission to neurones located in the dorsal horn or in the intermediate zone was depressed to a similar extent. The depression was readily antagonized by naloxone. Transmission from group Ia or Ib muscle afferents to neurones located in the intermediate zone was not affected, or was facilitated by DSLET. 3. The results show that DSLET has similar depressive actions on spinal neurones to monoamines, but its actions are more widespread. Like monoamines it affects transmission from nociceptors and group II muscle afferents, but in addition it gates transmission from low-threshold cutaneous afferents. Furthermore its effects do not appear to be restricted to interneurones at particular locations since it depressed responses of dorsal horn interneurones (gated by serotonin) as well as intermediate zone interneurones (gated by noradrenaline).
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PMID:A leu-enkephalin depresses transmission from muscle and skin non-nociceptors to first-order feline spinal neurones. 970

Pharmacological, neurochemical and behavioural findings support a possible role of endogenous opioids in clinical depression. There is evidence from animal studies that delta-opioid receptors are involved in several behavioural responses to opioids, including motivational activities. In the present study, the mixed enkephalin catabolism inhibitor, RB 101 (N(R,S)-2-benzyl-3[(S)-(2-amino-4-methylthiobutyldithio]-1-oxoprop yl)-L-phenylalanine benzyl ester) (1.25, 2.5 and 5 mg/kg), induced a dose-dependent antidepressant-like effect in a learned helplessness model. Thus, RB 101 reversed escape deficits in rats previously subjected to inescapable shocks, suggesting the involvement of endogenous enkephalins in depression. Similar effects were observed after administration of the selective delta-opioid receptor agonist, BUBU (Tyr-D.Ser-(O-tert-butyl)-Gly-Phe-Leu-Thr(O-Tet-butyl-OH) (1 and 2 mg/kg). Moreover, RB 101 effects were antagonized by administration of naltrindole (NTI) (0.1 mg/kg), which points to a preferential involvement of delta-opioid receptors in this enkephalin-controlled behaviour. As RB 101 has been reported to be almost devoid of opiate-related side-effects, it could represent a promising alternative in the treatment of depressive patients who are unresponsive to, or intolerant of, classical antidepressants.
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PMID:Involvement of delta-opioid receptors in the effects induced by endogenous enkephalins on learned helplessness model. 972 24


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