Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0010200 (cough)
 23,843 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Uniform design method was employed to optimize the mobile phase of HPLC in order to determine simultaneously three principal components, theophylline, amobarbital and methylephedrine hydrochloride, in Chuanjing tablet, a compound preparation for asthma and cough. The stationary phase was ODS and the optimal mobile phase composition was V (0.015 mol/L phosphate buffer containing 0.3% triethylamine, pH 4.9): V (methanol) = 35:65. The detection was performed at 215 nm and the sensitivity was time programmed for simultaneous determination of minor and major components. Caffeine was selected as the internal standard. A baseline separation was achieved within 10 min. The linear ranges for theophylline, amobarbital and methylephedrine hydrochloride were 0.03 g/L-0.20 g/L, 7.5 mg/L-50.0 mg/L and 7.5 mg/L-50.0 mg/L, recoveries 99.7%-102.6%, 98.5%-100.2% and 98.0%-102.7%, inter-day RSDs 0.23%-1.2%, 0.35%-2.5%, 0.33%-1.6% respectively. This HPLC method is rapid and accurate, and suitable for the quality control of the preparation.
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PMID:[Determination of three principal components in chuanjing tablets by using high performance liquid chromatography]. 1254 8

Derivative spectrophotometric procedures and an isocratic high performance liquid chromatographic method for the determination of butamyrate citrate (Sinecod, Safarol) in cough syrups have been developed. In the spectrophotometric method, direct measurement of the drug at its absorption maxima is impossible because of interference from different absorbing excipients. Extraction of butamyrate citrate was performed with n-pentane/isopropyl alcohol. Quantification was carried out through the use of 1D derivative at a trough depth of 253.6 nm where interferences from other coextracted compounds are negligible. The extraction efficiency expressed as a % recovery and precision were assessed by fortifying placebo syrup(s) with known amounts of the compound. Also, a reversed phase high performance liquid chromatographic method was used with a mobile phase containing 0.015 M aqueous tetraethylammonium hydrogen sulfate, methanol and acetonitrile 40:30:30 adjusted to pH 3.50 with ammonium hydroxide. The retention behavior of butamyrate citrate as a function of both pH and salt concentration in the aqueous portion of the mobile phase was investigated. Quantification was achieved with UV detection at 258 nm based on peak area. The HPLC method clearly separates the analyte from its degradation products derived after storage of samples under different stress conditions such as acid, alkaline, temperature, oxygen and light. The described methods were successfully applied to the determination of butamyrate citrate in commercial pharmaceutical products and in placebo syrups prepared in the laboratory with good accuracy and precision. The results of the present study show that the use of the derivatives and the HPLC procedure provide precise and sensitive methods for the determination of the compound in pharmaceutical formulations.
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PMID:Determination of butamyrate citrate in cough preparations by derivative UV spectrophotometry and high performance liquid chromatography. 1272 92

The present study was carried out to evaluate the antitussive potential of methanol extract of Ionidium suffruticosam Ging. (Violaceae) which was investigated for its effect on a cough model induced by sulfur dioxide gas in albino mice. It exhibited significant dose-dependent antitussive activity when compared with the control, 250 and 500 mg/kg (p.o.) of the extract showing 28.37% and 54.16% inhibition of the cough with respect to control group. The antitussive activity of the extract was comparable to that of codeine phosphate, a prototype antitussive agent.
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PMID:Evaluation of antitussive potential of Ionidium suffruticosam Ging. (Violaceae) extract in albino mice. 1291 92

The methanol extract of A. webbiana Lindl was evaluated for its effect on a cough model induced by sulphur dioxide gas in mice. When administered orally it exhibited significant antitussive activity compared with the control in a dose dependent manner. The antitussive activity of the extract was compared with that of codeine phosphate, a prototype antitussive agent. The A. webbiana leaf extract (400 and 600 mg/kg) showed maximum inhibition of cough frequency by 71.69% and 78.67%, respectively, when compared with the control group and was comparable in effect to codeine phosphate.
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PMID:Antitussive activity of Abies webbiana Lindl. leaf extract against sulphur dioxide-induced cough reflex in mice. 1368 Aug 27

The methanol extract of Ficus racemosa Linn (Moraceae) (stem bark) (MEFR) was tested for its antitussive potential against a cough induced model by sulphur dioxide gas in mice. The extract demonstrated significant (p < 0.001) antitussive activity at all tested dose levels when compared with the control. The antitussive activity of the extract was comparable to that of codeine phosphate (10 mg), a standard antitussive agent. The extract exhibited maximum inhibition of 56.9% at a dose of 200 mg/kg (p.o.) 90 min after administration.
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PMID:Antitussive potential of methanol extract of stem bark of Ficus racemosa Linn. 1459

From the polar portion of the methanol extract of thyme (leaf of Thymus vulgaris; Labiatae), which has been used as a stomachic, carminative, a component of prepared cough tea, and as a spice, a new hydroxyjasmone glucoside, (Z)-5'-hydroxyjasmone 5'-O-beta-D-glucopyranoside was isolated together with five related compounds and four aromatic compounds. The structures of the new compound was clarified by spectral investigation.
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PMID:A new hydroxyjasmone glucoside and its related compounds from the leaf of thyme. 1530 5

The methanol extract of the leaves of Lagerstroemia parviflora Roxb was investigated for its effect on a cough model induced by sulfur dioxide gas in mice. It exhibited significant antitussive activity when compared with the control in a dose-dependent manner. The L. parviflora extract (100, 200, 300 mg/kg) showed maximum inhibition of cough reflex at 90 min after drug administration and the antitussive activity was comparable to that of codeine phosphate, a standard antitussive agent.
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PMID:Evaluation of antitussive activity of Lagerstroemia parviflora leaf extract. 1547 90

From the polar portion of the methanol extract of thyme (leaf of Thymus vulgaris; Labiatae), which has been used as an important stomachic, carminative, a component of prepared cough tea, and a spice, seven monoterpenoid glycosides were isolated together with two known monoterpenoids and three known monoterpenoid glucosides. Structures of the seven monoterpenoid glycosides were determined by spectral analysis.
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PMID:Monoterpenoids and their glycosides from the leaf of thyme. 1556 Nov 94

Daucus carota L. (Family: Apiaceae alt. Umbelliferae), commonly known as 'wild carrot' or 'Queen Anne's-lace,' is an ecologically invasive erect biennial naturalized to Scotland. The ethnobotanical uses of this species include applications in the treatment of cough, diarrhea, dysentery, cancer, malaria and tumors, and as an antiseptic, abortifacient, aphrodisiac, carminative, stimulant, stomachic and tonic. The major constituents isolated from the methanol extract of D. carota seeds by reversed-phase preparative high performance chromatography were luteolin, luteolin 3'-O-beta-D-glucopyranoside and luteolin 4'-O-beta-D-glucopyranoside, three flavones. The constituents were assessed for their antibacterial and free radical scavenging activities, as well as toxicity towards brine shrimp. Among these three flavones, luteolin showed the highest degree of free radical scavenging activity (RC50 = 4.3 x 10(-4) mg/mL) in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Both luteolin and its 4'-O-glucoside demonstrated bactericidal activity against Staphylococcus aureus and Escherichia coli (Minimum Inhibitory Concentration [MIC] = 5.0 x 10(-2) - 1.0 x 10(-1) mg/mL). Luteolin also demonstrated antibactericidal activity against Bacillus cereus and Citrobacter freundii (MIC = 5.0 x 10(-2) mg/mL). Luteolin 3'-O-glucoside showed bactericidal activity against Bacillus cereus and Lactobacillus plantarum (MIC = 2.5 x 10(-1) mg/mL and 5 x 10(-1) mg/ mL, respectively). In the brine shrimp lethality assay, the LD50 value of luteolin was 5.3 x 10(-2) mg/mL, and that of its 3'-O-glucoside and 4'-O-glucoside were > 1.0 mg/mL.
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PMID:The assessment of biological activities associated with the major constituents of the methanol extract of 'wild carrot' (Daucus carota L) seeds. 1609 36

Argemone platyceras is used in Mexico as a remedy for cough, bronchitis and pneumonia. The present study was performed to investigate the pharmacological anti-asthmatic properties of Argemone platyceras on airways and to identify its active principles. Methanol extracts of leaves and flowers, subsequent organic and aqueous extraction phases, and silica gel chromatography fractions were assayed on the carbachol-induced response, and/or on ovalbumin antigenic challenge, and on leukotriene D(4)-induced response of tracheae from sensitized and non-sensitized guinea-pigs. Methanol extracts, ethyl-acetate phase, and its fractions 6 and 7 inhibited the carbachol-induced contractile response. Isoquercitrin and rutin were the main compounds found in fractions 6 and 7 respectively. Isoquercitrin (fraction 6) abolished the response to ovalbumin, and decreased the contractile response to leukotriene D(4). Because of its effect on carbachol-induced contractile response, on the late-phase response to ovalbumin, and on leukotriene D(4)-induced contractile response, isoquercitrin might be highly useful in treatment of asthma.
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PMID:Isoquercitrin from Argemone platyceras inhibits carbachol and leukotriene D4-induced contraction in guinea-pig airways. 1620 93


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