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Query: UMLS:C0009443 (cold)
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Yersinia enterocolitica and Y. enterocolitica-like bacteria were frequently isolated from samples of both raw bulked milk (34/150) and farm bottled (raw) milk (5/20). These bacteria were also found to contaminate creamery pasteurized milk (6/100 samples) and farm pasteurized milk (4/50 samples). Although Y. enterocolitica was the most commonly isolated species, Y. intermedia and Y. frederiksenii were also frequently obtained (52, 31 and 15% of isolates, respectively). Also, one atypical strain was identified as Y. aldovae. The Y. enterocolitica strains were largely biotype 1 (20/27) including five strains which could ferment lactose. One third of the Y. enterocolitica strains were not typable, but of those which were, the serotypes were 0:34 (18.5%), 0:5.27 (18.5%), 0:6.3 (15%), 0:4 (11%) and 0:7 (4%). Pre-enrichment in trypticase-soy broth (TSB) (at 22 degrees C for 24 h) followed by selective enrichment in bile-oxalate-sorbose broth (at 22 degrees C for 6 d) allowed the recovery of 92.3% of all isolates, as compared with 15.4% using cold enrichment in TSB at 4 degrees C for 21 d.
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PMID:The incidence of Yersinia enterocolitica and Yersinia enterocolitica-like organisms in raw and pasteurized milk in Northern Ireland. 377 12

A total of 1,203 unselected routine faecal samples from 1,006 patients were cultured for Yersinia species by a cold enrichment technique. Seventy-five specimens (6.1%) from 63 patients were culture-positive for Yersinia spp. Fifty-two were Yersinia enterocolitica, 22 Yersinia frederiksenii and 1 Yersinia intermedia. The predominant Y. enterocolitica isolates belonged to biotype 1 - serotype 0:6, 30 or serotype 0:5, 27. Y. frederiksenii strains were non-typable. Forty isolates were recovered from 33 patients with gastroenteritis. During the study period 83 Salmonella spp. from 33 patients, 17 Shigella sonnei from 13 patients and 13 Campylobacter jejuni from 12 patients were cultured. Yersinia spp. was isolated in association with salmonella on three occasions, twice with rotavirus and once each with Shigella sonnei, Campylobacter jejuni and Trichuris trichiura.
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PMID:Faecal carriage rate of Yersinia species. 378 82

A study of 208 children aged under 15 hospitalized with diarrhea and 108 matched controls was conducted at Chiang Mai University Hospital, Chiang Mai, Thailand from May 1983 to April 1984 to investigate the incidence, seasonal distribution, and causative agents of diarrhea. 1 or more enteropathogens were isolated from 121 (58%) diarrhea patients and from 32 (30%) controls. In diarrhea patients, Shigella spp. were most frequently isolated (22%) followed by enteropathogenic Escherichia coli (EPEC) (20%), rotavirus (18%), Campylobacter jejuni (7%), Salmonella (4%), Aeromonas (1%) and Plesiomonas shigelloides (1%). No Yersinia enterocolitica were found. Pathogens such as Norwalk agent, adenovirus, and enterotoxigenic E. coli (ETEC) were not sought. A concurrent study revealed ETEC in 3.2% of diarrheal children and 1.5% of controls. Parasites were identified in 11% of diarrhea patients; Giardia lamblia was most commonly found. In 32% of positive diarrhea and 19% of positive control cases more than 1 pathogen was isolated. Shigella was the least involved in cases with multiple infections. Only Shigella and rotavirus were found significantly more often in diarrhea cases than controls and their relation with age, season, and clinical presentation are reported. A high incidence of EPEC among control patients suggests common asymptomatic carriage. 2 diarrhea peaks occurred in the hot and cold seasons; the peak of Shigella diarrhea occurs in the summer, and may be related to the shortage of water at this time of year. Both shigellosis and rotavirus diarrhea patients had an acute onset of symptoms: cramps, convulsions, mucoid and bloody stools with high numbers of white and red blood cells were signficantly more common in shigellosis patients.
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PMID:The relative importance of various enteropathogens as a cause of diarrhoea in hospitalized children in Chiang Mai, Thailand. 379 60

A patient with fatal Yersinia enterocolitica sepsis was seen recently in our intensive care unit. The patient had received two units of packed red blood cells during a surgical procedure. Cultures of the donor blood yielded Y enterocolitica, and a whole-organism enzyme-linked immunosorbent assay of the donors' sera suggested a recent infection with Y enterocolitica in an asymptomatic donor. Though rare, Y enterocolitica, which can grow at the cold temperatures of refrigerated blood, should be considered as a possible source of sepsis following blood transfusion.
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PMID:Fatal Yersinia enterocolitica sepsis after blood transfusion. 384 Mar 56

From 134 samples of raw or cooked vegetables taken in two points of a cold line we isolated 19 strains of Yersinia enterocolitica, 3 of Y. intermedia 3 of Y. kristensenii. None of them belongs to serotypes usually pathogenic for man. The raw vegetables are most contaminated. Y. kristensenii and Y. intermedia were isolated especially from samples of green salad. The used method was a cold enrichment at 4 degrees C followed by a potassium hydroxyde treatment. The activity of 16 antimicrobial drugs was studied by the disk diffusion test at 28 degrees C. All strains were sensitive to aminoglycosides, minocycline, colistine and cotrimoxazole. The results obtained with chloramphenicol and beta-lactam antibiotics vary in terms of species and/or biotypes.
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PMID:[The search for Yersinia enterocolitica in vegetables treated by a cold line in school cafeterias in Montpellier]. 403 45

The occurrence of Yersinia enterocolitica and related species (Y. intermedia, Y. frederiksenii, Y. kristensenii) in foods from France was investigated by using different enrichment procedures. Initially, seven procedures were evaluated with pork products. These methods included a cold preenrichment in yeast extract-rose bengal broth or in phosphate-sorbitol-bile medium, followed by selective enrichment either in Pastone-sucrose-Tris-azide broth, in modified Rappaport broth, or in bile-oxalate-sorbose broth, and then isolation onto Hektoen or cefsulodin-irgasan-novobiocin agar with or without KOH pretreatment. The best enrichment procedure in terms of percentage of positive samples obtained within the shortest time was the combination of phosphate-sorbitol-bile and bile-oxalate-sorbose with alkali treatment before isolation onto cefsulodin-irgasan-novobiocin agar. This system was then used to analyze foods other than pork. An average contamination rate of 33.5% was observed for 666 samples analyzed; pork products were by far the most contaminated, especially the so-called tartinette (96.8% of positive samples) which contained up to five different strains of Yersinia spp. Environmental serogroups of Y. enterocolitica O:5, O:39,41, O:6, and O:7,8 were predominant, but no isolate of either human pathogenic type (O:3 or O:9) was obtained.
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PMID:Isolation of Yersinia enterocolitica and related species from foods in France. 408 78

The purpose of this study was to determine the presence of zoonotic pathogens simultaneously in animals. The isolation of human pathogenic Yersinia enterocolitica (Ye), Yersinia pseudotuberculosis (Yp), Campylobacter jejuni (Cj), Salmonella spp. (Sal) and Leptospira spp. (Lep) in 318 cats and 252 dogs were performed in Shimane Prefecture, Japan. A total of 13 isolates of Yp (4 strains) and Sal (9 strains) were recovered from intestine and/or mesenteric lymph nodes (MLN) of 13 cats (4.1%) but not Ye, and Cj was not examined. A total of 88 isolates of Ye (15 dogs, 15 strains), Yp (16 dogs, 16 strains), Cj (10 dogs, 13 strains) and Sal (39 dogs, 44 strains) were recovered from intestine and/or MLN of 76 dogs (30.2%). Two species of Ye O3, Cj and Sal were recovered from either intestine or MLN of 4 dogs but not from cats. Lep was not detected in dogs and cats kidney. The 101 isolates from dogs and cats belonged to Ye 3B/O3/II (biotype/serotype/phage type) (2 strains), 4/O3/VIII (10 strains) and 2/O5,27 (3 strains), Yp serotypes 1b, 2b (each 3 strains), 2c (2 strains), 4a (2 strains), 4b (4 strains), 5a (5 strains) and 7 (1 strain), Cj serotypes TCK 9, 13, 26 (each 1 strain), 21 (2 strains) and untypable (8 strains) and 24 serotypes of Sal. Ye O3 and Yp were detected frequently in cold months. There was no definite seasonal variation of Ye O5,27 Cj and Sal in internal origin of dogs and cats. Ye O3, Yp and Cj were counted at 10(2) to 10(7) cells per g of the jejunal-to-rectal contents, but Sal at less than 10(2) cells per g of the intestinal contents. Ye O3, Yp and Sal were recovered from mesenteric lymph nodes, but not Ye O5,27 and Cj.
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PMID:Presence of zoonotic pathogens (Yersinia spp., Campylobacter jejuni, Salmonella spp., and Leptospira spp.) simultaneously in dogs and cats. 409 52

During the period July 1980 through June 1983, in a General Hospital in Madrid, the following organisms were detected from 6,970 patients with gastroenteritis: 710 Salmonella spp. 506 Campylobacter jejuni, 379 Shigella spp., 12 Yersinia enterocolitica, 1,466 rotavirus, 134 Giardia lamblia, and 4 Entamoeba histolytica. Chloramphenicol showed good activity against most tested strains of Salmonella spp., Shigella spp., and C. jejuni. The incidence of Salmonella spp. and Shigella spp. was very marked in the hot dry months of the year, rotavirus predominated during the cold months, and no seasonal variations of importance were seen for C. jejuni and G. lamblia.
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PMID:Three-year prospective study of intestinal pathogens in Madrid, Spain. 609 18

Yersinia pseudotuberculosis infection was diagnosed in 12 children on the basis of recovery of the organism from stool cultures and a 4-fold or greater titer change in agglutinating antibody. Eight of the 12 Yersinia isolates were recovered from stool cultures only after cold enrichment. Clinical findings in 50% or more of patients were fever, rash, diarrhea, desquamation, strawberry tongue, vomiting, red and cracked lips, abdominal pain, arthralgias, hepatomegaly and conjunctivitis. The patients' clinical manifestations and courses of illness resembled those of Izumi fever, an illness that occurs epidemically in Japan. Additionally the finding in two children fulfilled the strict criteria for Kawasaki syndrome, and signs in the other 10 children were consistent with that diagnosis.
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PMID:Yersinia pseudotuberculosis infection in children, resembling Izumi fever and Kawasaki syndrome. 634 44

2 outbreaks of acute fatal enteric disease involving 20 animals in a breeding unit of approximately 200 cynomolgus monkeys were diagnosed as yersiniosis; Yersinia pseudotuberculosis was isolated from 50% of the clinically affected animals. Post-mortem findings included enlarged mesenteric lymph nodes with some enterocolitis and necrotic foci in liver and spleen. Approximately 7% of clinically healthy monkeys were found to be excreting Y. pseudotuberculosis and a further 5% Y. enterocolitica. Rectal swabs, though less convenient, were better than faecal samples for the detection of Yersinia spp. in 'healthy' monkeys. Efficiency of the cold saline technique and direct plating for isolating Yersinia spp. were compared. It is thought likely that the infection was introduced into the unit by asymptomatic infected monkeys.
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PMID:Yersiniosis in a breeding unit of Macaca fascicularis (cynomolgus monkeys). 634 31


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