Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0008272 (chlorosis)
 2,195 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of the tetrapyrrole biosynthesis inhibitor gabaculine on the expression of specific genes involved in phycocyanin biosynthesis was investigated in cultures of Synechococcus PCC6301 in nitrogen chlorosis, and during recovery to nitrogen sufficiency. Patterns of transcription of the cpcBA (phycocyanin subunits), hemL (glutamate semialdehyde aminotransferase) and hemB (aminolaevulinate dehydratase) genes were visualised by Northern blotting and gene product formation for cpcBA, hemL and the gene for glu tRNA synthetase were monitored by Western blotting. Inhibition of phycobilin biosynthesis by gabaculine greatly decreased production of phycocyanin protein and of cpcBA transcript, indicating a tight coordination of apoprotein biosynthesis with chromophore supply at the level of transcription. Different patterns of response were observed with the other genes at the level of transcript formation or gene product synthesis.
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PMID:Expression of genes involved in phycocyanin biosynthesis following recovery of Synechococcus PCC 6301 from nitrogen starvation, and the effect of gabaculine on cpcBa transcript levels. 866 6

Pierce's disease (PD, Xylella fastidiosa) of grapevine is the primary pathogen limiting vinifera grape production in Florida and other regions of the southeastern United States. Quick and accurate detection of PD strains is essential for PD studies and control. A unique random amplified polymorphic DNA (PD1-1-2) was isolated from a PD strain from Florida. Fragment PD1-1-2 was cloned, sequenced, and found to be 1005 bp in length. PCR primers were designed to utilize these sequence data for PD strain detection. One primer set (XF176f-XF954r) amplified a 779-bp DNA fragment from 34 PD strains including seven pathotypes of X. fastidiosa, but not from strains of Xanthomonas campestris pv. campestris, Xan. vesicatoria or Escherichia coli. A second primer set (XF176f and XF686r) amplified a 511-bp fragment specific to 98 PD strains, but not from strains of citrus variegated chlorosis, mulberry leaf scorch, oak leaf scorch, periwinkle wilt, phony peach, or plum leaf scald. Sequence analysis indicated that RAPD fragment PD1-1-2 contains a Ser-tRNA gene. The PD-specific region includes a TaqI restriction site (TCGA) and is 150 bp downstream of the Ser-tRNA gene.
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PMID:Specific detection of Xylella fastidiosa Pierce's disease strains. 1039 32

In eukaryotic cells, RNA polymerase III is highly conserved and transcribes housekeeping genes such as ribosomal 5S rRNA, tRNA and other small RNAs. The RPC5-like subunit is one of the 17 subunits forming RNAPIII and its exact functional roles in the transcription are poorly understood. In this work, we report that virus-induced gene silencing of transcripts encoding a putative RPC5-like subunit of the RNA Polymerase III in a model species Nicotiana benthamiana had pleiotropic effects, including but not limited to severe dwarfing appearance, chlorosis, nearly complete reduction of internodes and abnormal leaf shape. Using transcriptomic analysis, we identified genes and pathways affected by RPC5 silencing and thus presumably related to the cellular roles of the subunit as well as to the downstream cascade of reactions in response to partial loss of RNA Polymerase III function. Our results suggest that silencing of the RPC5L in N. benthamiana disrupted not only functions commonly associated with the core RNA Polymerase III transcripts, but also more diverse cellular processes, including responses to stress. We believe this is the first demonstration that activity of the RPC5 subunit is critical for proper functionality of RNA Polymerase III and normal plant development.
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PMID:Virus-induced gene silencing of the RPC5-like subunit of RNA polymerase III caused pleiotropic effects in Nicotiana benthamiana. 2728 27

Polymerase chain reaction amplification of DNA from various strains of Xylella fastidiosa with tRNA consensus primers produced three different fingerprint groups. The citrus variegated chlorosis (CVC) and mulberry leaf scorch strains were unique and readily separated from each other and all other strains tested. Internal primers were designed based on the sequence of a DNA fragment unique to the CVC strain. An assay was developed with a mixture of these primers and those reported to detect 18 strains of X. fastidiosa. The assay was used to survey citrus in Brazil. The strain identified to be the cause of CVC was found in constant association with trees with CVC symptoms. On occasion, trees with no symptoms were found to have the CVC strain; this was presumably due to presymptomatic infections. No other strains were found in this survey, and X. fastidiosa was not associated with citrus blight.
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PMID:A Survey for Strains of Xylella fastidiosa in Citrus Affected by Citrus Variegated Chlorosis and Citrus Blight in Brazil. 3089 79