Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0008272 (chlorosis)
 2,195 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In wild-type Nicotiana plumbaginifolia and other higher plants, nitrate reductase (NR) is rapidly inactivated/activated in response to dark/light transitions. Inactivation of NR is believed to be caused by phosphorylation at a special conserved regulatory Ser residue, Ser 521, and interactions with divalent cations and inhibitory 14-3-3 proteins. A transgenic N. plumbaginifolia line (S(521)) was constructed where the Ser 521 had been changed by site-directed mutagenesis into Asp. This mutation resulted in complete abolishment of inactivation in response to light/dark transitions or other treatments known to inactivate NR. During prolonged darkness, NR in wild-type plants is in the inactivated form, whereas NR in the S(521) line is always in the active form. Differences in degradation rate between NR from S(521) and lines with non-mutated NR were not found. Kinetic constants like Km values for NADH and NO3(-) were not changed, but a slightly different pH profile was observed for mutated NR as opposed to non-mutated NR. Under optimal growth conditions, the phenotype of the S(521) plants was not different from the wild type (WT). However, when plants were irrigated with high nitrate concentration, 150 mM, the transgenic plants accumulated nitrite in darkness, and young leaves showed chlorosis.
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PMID:Mutation of the regulatory phosphorylation site of tobacco nitrate reductase results in constitutive activation of the enzyme in vivo and nitrite accumulation. 1294 Sep 50

A plant can respond to the threat of a pathogen through resistance defenses or through tolerance. Resistance has been widely studied in many host pathogen systems but little is known about genetic changes which underlie a tolerant interaction. A recently developed model system for a tolerant tomato (Lycopersicon esculentum Mill) interaction with a fungal wilt pathogen, Verticillium dahliae Kleb, is examined with respect to changes in gene expression and compared to a susceptible infection. The results indicate that genetic changes can be dramatically different and some genes that are strongly elevated in the susceptible interaction are actually down-regulated in tolerance. Similar levels of fungal DNA and an up-regulation of many pathogenesis related genes indicate that in both types of interaction the presence of fungus is clearly recognized by the plant but other changes correlate with the absence of symptoms in the tolerant interaction. For example, a gene encoding a known 14-3-3 regulatory protein and a number of genes normally affected by this protein are down-regulated. Furthermore, genes which may contribute to foliar necrosis and cell death in the susceptible interaction also appear to be suppressed in the tolerant interaction, raising the possibility that the wilt symptoms, chlorosis and necrosis which are observed in the susceptible interaction, are actually programmed to further limit the growth of the fungal pathogen, and protect the general tomato population.
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PMID:Gene suppression in a tolerant tomato-vascular pathogen interaction. 1730 29