Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0008272 (chlorosis)
 2,195 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Stunting, chlorosis, and light yellow mottling resembling symptoms of nutrient deficiency were observed in angelonia (Angelonia angustifolia) in commercial production in New York. Numerous, filamentous particles 520 to 540 nm long and spherical virus particles 30 nm in diameter were observed by transmission electron microscopy (TEM) in negatively stained partially purified extracts of symptomatic Angelonia leaf tissue. Two viruses, the filamentous potexvirus Alternanthera mosaic virus (AltMV) and the spherical carmovirus Angelonia flower break virus (AnFBV) were subsequently identified on the basis of nucleotide sequence analysis of amplicons generated by reverse transcription (RT)-PCR using total RNA isolated from infected leaf tissue. A 584-bp portion of the replicase-encoding region of the AltMV genome was obtained with the degenerate primers Potex 2RC (5'-AGC ATR GNN SCR TCY TG-3') and Potex 5 (5'-CAY CAR CAR GCM AAR GAT GA-3') (3). Forward (AnFBV CP 1F-5'-AGC CTG GCA ATC TGC GTA CTG ATA-3') and reverse (AnFBV CP 1R-5'-AAT ACC GCC CTC CTG TTT GGA AGT-3') primers based on the published AnFBV genomic sequence (GenBank Accession No. NC_007733) were used to amplify a portion of the viral coat protein (CP) gene. The nucleotide sequence of the amplicon generated using the potexvirus-specific primers (GenBank Accession No. EU679362) was 99% identical to the published AltMV (GenBank Accession No. NC_007731) sequence and the nucleotide sequence of the amplicon obtained using the AnFBV CP primers was 99% identical to the published AnFBV genomic sequence (GenBank Accession No. EU679363). AnFBV occurs widely in angelonia (1) and AltMV has been identified in phlox (2). These data confirm the presence of AltMV and AnFBV in diseased angelonia plants showing stunting and nutrient deficiency-like symptoms and substantiates, to our knowledge, this first report of AltMV in angelonia in the United States. References: (1) S. Adkins et al. Phytopathology 96:460, 2006. (2) J. Hammond et al. Arch. Virol. 151:477, 2006. (3) R. A. A. van der Vlugt and M. Berendeson. Eur. J. Plant Pathol. 108:367, 2002.
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PMID:First Report of Alternanthera mosaic virus Infection in Angelonia in the United States. 3076 47

During the 2004 growing season in the Liaoning Province in China, where there was large population of whiteflies, several sweet potato (Ipomoea batatas) breeding lines showed leaf curl symptoms. A survey was conducted to determine the incidence of Sweet potato leaf curl virus (SPLCV) in China. Sixteen plants were collected and stem scions from those plants were graft inoculated to Ipomoea nil. Three weeks later, the indicator developed symptoms of leaf curling, interveinal chlorosis, and stunting. Total nucleic acid was extracted from young leaves of sweet potato and then evaluated using polymerase chain reaction (PCR). Primers, developed by Briddon and Markham (1) and used as universal primers for amplification of the geminivirus DNA fragment, were BM-V (5'-KSG GGT CGA CGT CAT CAA TGA CGT TRT AC-3') and BM-C (5'-AAR GAA TTC ATK GGG GCC CAR ARR GAC TGG C-3'). Amplified fragments with BM primers theoretically should have sizes almost equal to the full length of the DNA A component of the bipartite genome (2). Expected DNA fragments of 2.8 kb that contained the AV1, AV2, AC1, AC2, AC3, and AC4 open reading frames were obtained from symptomatic, but not from symptomless (uninfected) plants. The 2.8-kb fragments obtained by amplification were purified and cloned into the PMD18-T vector. Recombinant plasmids were then transformed into competent cells of Escherichia coli strain DH5(. The fragment was sequenced (GenBank Accession No. DQ512731), and nucleotide sequence of corresponding regions were compared with a published sequence of SPLCV available in GenBank (Accession No. AF104036). The AC4 and AC2 genes showed the highest (92%) and the lowest (83%) identity, respectively. This virus has been reported in the United States, Taiwan, Japan, and Peru. To our knowledge, this is the first report of the natural occurrence of SPLCV in China. References: (1) R. W. Briddon and P. G. Markham. Mol. Biotechnol. 1:202, 1994. (2) M. Onuki and K. Hanada. Ann. Phytopathol. Soc. Jpn. 64:116, 1998.
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PMID:First Report of Sweet potato leaf curl virus in China. 3078 25