Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0007097 (carcinoma)
 152,788 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

HPV DNA and HSV-2 antigens were detected in a series of cervical lesions by DNA hybridization and PAP stain techniques in order to investigate the possible role of HPV and HSV-2 in the viral etiology of cervical carcinoma. The correlation of HPV DNA sequence and HSV-2 antigens was analyzed. The results showed that the positive rates of HPV 16, 18 and 11 in cervicitis, dysplasia and cervical cancer were 13%; 0, 13%; 17%, 17%, 17%; 43%, 8%, 4%; and HSV-2 antigens were found in 41% of cervicitis, 50% of cervical dysplasia and 63% of cervical carcinoma. Comparing the results of HPV hybridization with that of HSV-2 antigens, the authors found that both HPV DNA and HSV-2 antigens could be detected in some cases. The results indicate that the viral etiology of cervical carcinoma may be multifactorial and HSV-2 and HPV may be synergistic in the development of cervical carcinoma.
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PMID:[Correlation of human papilloma virus (HPV) and herpes simplex virus-2 (HSV-2)in the viral etiology of carcinoma of uterine cervix]. 255 61

Routinely paraffin-embedded sections of dysplasia, carcinoma in situ (CIS) and invasive (squamous) carcinoma of the cervix were studied to determine the participation of human papilloma virus (HPV) in these tissues. Morphological observation (1,059 cases) revealed condylomatous changes to reach 54% in dysplasia, 25% in CIS and 25% in invasive carcinoma. Condylomatous changes were also found to be 25 to 40% in the non-cancerous epithelia adjacent to in situ or invasive carcinomas. The immuno-peroxidase-PAP-method using anti-HPV serum was applied to 98 selected sections in which condylomatous changes were morphologically observed. HPV antigens were found to reach 56% in dysplasia, 42% in CIS and 35% in invasive carcinoma, and this result suggested that the morphologically observed condylomatous changes did not always coincide with virus maturation in the infected cells. By means of the in situ hybridization technique, HPV type-6, -11, -16 and -18 DNAs were all detected in dysplasia sections, whereas HPV type-16 DNA was demonstrated distinctively at a high rate among in situ and invasive carcinomas.
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PMID:[Human papilloma virus (HPV) antigens and DNA in dysplasia and carcinoma of the uterine cervix]. 255 25

Binding of 2 lectins--peanut agglutinin (PNA) and Dolichos biflorus agglutinin (DBA)--with human colonic carcinomas, adenomas, juvenile and hyperplastic polyps, and polyps in Cronkhite-Canada syndrome was examined histochemically by the lectin-antilectin-PAP method. With PNA, brush borders of the carcinoma cell were stained(90.9%), while the cytoplasm of the adenomas and polyps were stained granularly (88.0%). With DBA, the cytoplasm of the carcinomas was stained diffusely (72.7%), though 25% of the cases showed the fringed type cytoplasmic staining similar to adenomas, polyps and normal crypts. It appears important to recognize the differences in the staining patterns, especially in PNA, between carcinomas and other benign lesions, which apparently reflect changes of sugar residues during cancerization.
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PMID:[Binding of peanut agglutinin (PNA) and Dolichos biflorus agglutinin (DBA) to human colonic carcinomas, adenomas, and polyps: histochemical staining by the lectin-antilectin-PEP method]. 258 Jan 8

Subpopulations of infiltrating mononuclear cells in 51 resected pulmonary carcinoma specimens were studied with immunohistochemical technique (ABC and PAP methods). The major constituent of the mononuclears was T-cells, especially the OKT8+ cells (T suppressor/cytotoxic). The local infiltration of the mononuclears in pulmonary carcinoma was related to the prognosis of the case. The more the OKT8+ and B1+ lymphocytes infiltrated, the longer the survival (rate obtained) whereas, the infiltration of some kinds of plasma cells tended to have a negative correlation with the prognosis of the case. The significance of the mononuclears in pulmonary carcinoma is discussed.
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PMID:[The subpopulations and prognostic implication of mononuclear cells infiltration in pulmonary carcinoma]. 263 56

Carcinoembryonic antigen (CEA) was stained by the PAP immunoperoxidase method in cancerous and non-cancerous gastric mucosa of 40 patients (25 non-cancerous dyspeptic patients and 15 patients with gastric carcinoma). The pattern of CEA localization was apical or membranous-cytoplasmic and immuno-reactivity was mild (+), moderate (++) or intensive ( ). No CEA immunoreactivity was detected in normal gastric mucosa whereas it was marked in gastric mucosa of non-cancerous dyspeptic patients with chronic atrophic gastritis and dysplasia (intense). In patients with superficial gastritis and epithelial hyperplasia it was mild or absent. The CEA localization pattern was also apical in non-cancerous dyspeptic patients with microscopic changes, e.g. superficial or chronic atrophic gastritis, epithelial hyperplasia and dysplasia, and in non-cancerous mucosa and cancerous tissue of patients with well (G1) and moderately (G2) differentiated adenocarcinoma.
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PMID:Immunohistochemical detection of carcinoembryonic antigen (CEA) in non-cancerous and cancerous gastric mucosa. 266 22

Tumour markers are often circulating tumour-associated indicators of tumour development. As such they are not suitable for tumour screening and localization, but valuable as adjuncts for medical follow-up care of tumour patients, where their serum level alterations may anticipate the clinical detection of tumour behaviour by a lead time of 1 to 6 months before other methods. The following tumour may be controlled by established markers: endocrine tumours by NSE, calcitonin, parathormone, 5-HIAA, catecholamines/metabolites etc.; head-neck tumours: SCC, CEA; thyroid carcinoma: TG, calcitonin; lung cancer: CEA, NSE, SCC; liver cancer: AFP (PLC), CA 19-9 (cholangiocell.), CEA (secondary): biliary tract and pancreatic cancer: CA 19-9; colorectal carcinoma: CEA, CA 19-9; squamous cell carcinoma (ENT, oesophagus, anal): SCC; breast cancer: CEA and CA 15-3; ovarian cancer: CA 125 (epithelial), CA 19-9 (mucinous); germ cell tumours (ovary including trophoblastic tumours/testes): AFP and HCG; prostatic cancer: PAP and PSA; bladder cancer: TPA.
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PMID:[Clinical relevance of tumor markers]. 267 6

The antibody against a molecular weight 15,000 protein isolated from the breast cyst fluid (GCDFP-15) was applied to tissue from cutaneous tumors, especially to sweat gland tumors. This protein was purified from the breast cyst fluid; antisera was prepared in rabbits. Ninety six cases of cutaneous tumors were stained by the PAP method. Apocrine hydrocystoma, syringocystadenoma papilliferum, so-called mixed tumor of the skin, extramammary Paget's disease, microcystic adnexal carcinoma, and mucinous carcinoma of the skin were positively stained. Eccrine poroma, eccrine spiradenoma, syringoma, eccrine hydrocystoma, papillary eccrine adenoma, adenoid type of basalioma were negative. These observations indicate that the positively stained tumors have functioning seromucous cells, which are characteristic of apocrine gland cells and eccrine dark cells in the skin.
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PMID:[An immunohistochemical study of cutaneous tumors using an antibody to the breast cyst fluid protein (GCDFP-15)]. 268 27

Today, the immuncytochemical demonstration of estrogen receptors can be regarded as established. Recently, a monoclonal antibody (mab) against the progesterone receptor-complex became commercially available. The antibody Mi 60-10, developed by Milgrom and co-workers, is murine and principally directed against the progesterone receptor of the rabbit, but it also attacks an "epitop" of the human receptor. The Western-Blot analysis shows the antibody having several specific bands against T-47-D cells in the region of 110,000 dalton. To estimate the practical performance of this antibody, 73 primary breastcarcinomas were incubated with both the ER-ICA-Kit and the new "mab". The PAP method was used for visualisation. In a frozen section the mab Mi 60-10 proved as successful as the ER-ICA-Kit. However, in contrast to the ER-ICA-Kit, the mab Mi 60-10 is also successful in locating the receptor, also in formalin-fixed samples embedded in paraffin. This is also the case with blocks, which were stored for several years. In aspirated cytological samples the immunocytochemical demonstration of progesterone was just as successful as that of estrogen and comparable with results on the histological section. A satisfactory validity can be obtained today through the development of highly specific monoclonal antibodies against the progesterone receptor complex, with the complete receptor diagnosis of mammary carcinoma both immunocytochemically in frozen sections and additionally, of progesterone receptors in paraffin wax sections. The only current problems involve the exact quantification of the immunocytochemical results. It is predictable today, that the DCC-method, when compared to the immunocytochemical receptor analysis, will reduce in significance.
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PMID:[Initial experiences with a monoclonal antibody to the progesterone receptor complex]. 270 36

34 adenomas of the colon and adjacent flat mucosa were reexamined by morphological, histochemical and immunohistochemical means. Adenomas were grouped according to the degree of epithelial cell atypia: group 1 (dysplastic adenomas) showing mild and moderate dysplasia and group 2 (cancerous adenoma) with severe dysplasia and carcinoma. Morphological and secretive changes (hyperplasia with hypersialomucin secretion), considered typical of 'transitional mucosa', were constantly found in the adjacent and stalk mucosa. A panel of six lectins were tested using PAP and ABC system methods to compare amount and cytoplasmic localization of labelling sites with the morphological changes such as hyperplasia, dysplasia and carcinoma. All the tested lectins were reactive in up to 90% of adenomas as well as in adjacent mucosa. Positivity was unrelated to the severity of dysplasia and no preferential localization of labelled sites was shown in the adenoma groups. However, cytoplasmic distribution of reactivity was quite different in hyperplastic epithelium compared to dysplastic ones.
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PMID:Lectin histochemistry of colonic adenomas. 270 41

We experienced two primary lung cancer patients who showed high levels of CA19-9 (Carbohydrate Antigen 19-9). One case was small cell carcinoma and the other was adenocarcinoma. In contrast to malignancies of digestive organ origin, rare CA19-9 positive cases are reported in lung cancer. To get evidences that these lung cancer tissues were producing CA19-9, PAP stain (Peroxidase anti peroxidase method: Sternberger) was performed, and positive stains were obtained in tumors surgically removed from these two patients. Especially in a patient who showed very high level of CA19-9 (27,369 U/ml), almost every tumor cell was positively stained. From these findings, we suggest the importance of checking CA19-9 in patients with lung cancer.
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PMID:[A case of two patients with primary lung cancer secreting CA19-9]. 283 35


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