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Query: UMLS:C0006142 (breast cancer)
 160,383 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In order to find the reasons for the conflicting results depicted during the estradiol stimulations of cultured MCF7, breast cancer cells we investigated, besides cell counts, the cell surface transferrin receptor as an additional means of assessing the effect of estradiol. In this study we report results obtained using different culture conditions, i.e. short-term or long-term phenol-red withdrawn cells grown either in calf-serum supplemented media or defined media. Our results point out concurrent variations of cell counts and transferrin receptors when short-term phenol-red withdrawn cells were grown in defined media. Discrepancies were, however, observed when short-term phenol-red withdrawn cells were grown in serum-supplemented media or when long-term phenol-red withdrawn cells were grown in defined media. In both cases, only transferrin receptors account for estradiol stimulation. These results highlight the importance of transferrin receptor measurement in cultured breast cancer cell experiments and suggest cell kinetic perturbations due, in all likelihood, to serum factors or factors secreted by long-term phenol-red withdrawn cells.
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PMID:Involvement of cell surface transferrin receptor in the assessment of estradiol stimulating effect on cultured breast cancer cells. 338 52

In recent years nutritional status gained greater attention as a surgical risk factor. This study analyzes the frequency of malnutrition in surgical patients with solid and operable tumors, the relation to the type of tumor and stage of the disease. In addition, the clinical value of the measurements carried out is discussed. The analysis was performed in 100 cancer patients (34 gastric cancer, 56 colorectal cancer, and 10 breast cancer). The nutritional assessment included individual dietary habits, ideal weight/height, triceps skinfold, arm muscle circumference, creatinine-height index, serum protein, albumin, prealbumin, cholinesterase, transferrin, total peripheral lymphocytes, and skin tests. The results were compared with international standards or normal plasma concentrations respectively. Most patients suffered from an alternation of the nutritional parameters indicating malnutrition, mostly Kwashiorkor-Marasmus Mix. Patients with gastrointestinal cancer, especially gastric cancer showed more often a decline of the nutritional status than patients with breast cancer. Malnutrition became more severe with advanced disease. The parameters examined revealed varying significance with respect to the assessment of the nutritional status. Some measurements showed little clinical importance; the reasons are discussed.
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PMID:[Significance of the nutritional status of surgical patients]. 393 Sep 1

A transferrin receptor was demonstrated in tumor tissue from 10 patients with breast carcinoma and one patient with breast sarcoma. Binding studies were conducted by measuring the amont of 1251-transferrin binding to microsomal preparations of the tumor tissue. Elevated levels of specific transferrin binding were found in the tumors with a range of 11-35% of bound transferrin, whereas microsomes prepared from non-neoplastic breast tissue samples bound only 2.3% and 2.4% of the transferrin. Scatchard analysis of binding studies conducted with tissues from a breast cancer and from a breast sarcoma indicate that the receptor has a Ka = 9.0 x 10(8)M. The binding site is specific for transferrin, as studies show that non-radioactive transferrin displaced labelled transferrin, while human IgG and human albumin did not. The receptor-transferrin complex was precipitated from a detergent extract of the breast sarcoma with antiserum to human transferrin. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of the immunoprecipitate gave a polypeptide of M 90,000 daltons, which is of similar molecular weight found for the putative transferrin receptor in all of a series of human cultured cell lines previously examined.
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PMID:Demonstration of the transferrin receptor in human breast cancer tissue. Potential marker for identifying dividing cells. 627 15

Serum copper levels of 132 controls and 122 cancer patients (including Hodgkin's disease, lung cancer, breast cancer, leukemia, untreated patients, and patients in progression) were measured using both atomic absorption (AA) and electron paramagnetic resonance (EPR) techniques. The data pairs were compared using linear regression analysis, EPR versus AA, and all the data (controls and cancers) fit a single regression line with a squared correlation coefficient (r2) of 0.80. Comparison of the subpopulations revealed possible small differences, but none great enough to be of diagnostic value for individual patients. Thus, in a number of cases of practical interest, EPR-determined serum copper levels were essentially redundant with respect to AA measurements. Previous reports recommending the use and possible superiority of EPR for serum copper determinations appear not to have compared the EPR and AA techniques adequately or correctly. EPR serum copper measurements may yet provide unique data in these diseases, but a more detailed analysis of the spectral parameters will be required. EPR-determined serum transferrin levels are also reported.
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PMID:Comparison of electron paramagnetic resonance and atomic absorption serum copper measurements in human normal control and cancer patients. 630 84

We tested the ability of hormones and growth factors to enhance the colony formation in soft agarose of breast carcinoma using two human breast carcinoma cell lines, MCF-7 and MDA-MB231, MCF-7 could clone in a basal medium supplemented only by insulin, transferrin, prostaglandin F2 alpha, and fibronectin. Combining oestradiol, dexamethasone, insulin, transferrin, and triiodothyronine with a basal medium supplemented with 5% (v/v) foetal bovine serum (FBS) increased colony forming efficiency (CFE) two-to three-fold over the best obtained in serum supplemented medium without hormones. While optimal CFE was seen in the hormonally supplemented medium plus 5% FBS, clonal anchorage independent growth could also be obtained without serum for both cell lines by substituting 0.5-1% (v/v) bovine serum albumin (BSA) for FBS. Although CFE was enhanced with the addition of hormones, they did not substantially alter the in vitro chemosensitivity patterns of the cell lines to 8 cytotoxic drugs. Hormonally-supplemented medium with 5% FBS increased the CFE of a small number of fresh specimens of human breast cancer compared with medium supplemented with serum alone. The systematic study of requirements for the in vitro growth of human breast cancer may improve drug sensitivity testing by increasing our ability to grow this neoplasm in culture.
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PMID:Hormone supplemented media for cloning human breast cancer: increased colony formation without alteration of chemosensitivity. 635 59

We tested the ability of a serum-free medium containing insulin, transferrin, 17 beta-estradiol, dexamethasone, triiodothyronine, prostaglandin F2 alpha, and fibronectin (HBCA medium) to support the continuous growth and passage of five human breast carcinoma cell lines on a collagen matrix. Doubling times of the cell lines (20 to 44 hr) were similar in HBCA and serum-supplemented media. The gross morphology of the cell lines was not altered in the serum-free medium. Insulin, transferrin, and the collagen matrix were the most essential factors required for optimal growth of the cell lines. Estradiol appeared to stimulate the growth of cell lines, both with and without estrogen receptors. HBCA medium supplemented with low concentrations of bovine serum albumin, Fraction V (0.5%, v/v), supported the clonal growth of three cell lines in soft agarose with colony-forming efficiencies superior to that observed with standard serum-supplemented medium. Deleting estradiol from HBCA medium reduced the colony-forming efficiency of the three cell lines. HBCA medium may be useful in studying hormonal regulation and improving the in vitro growth of human breast cancer.
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PMID:Continuous culture and soft agarose cloning of multiple human breast carcinoma cell lines in serum-free medium. 646 10

Serum haptoglobin, transferrin and albumin were studied electrophoretically in 50 patients with carcinoma of breast and in 50 normal healthy women. Results on haptoglobin show a preponderance of products of gene Hp1 (0.26) in patients as compared to controls (0.14). No variation was observed at the transferrin locus in either sample. The most notable finding was the detection of four cases of alloalbuminaemia (8%) in breast cancer Patients. The high incidence of alloalbuminaemia prompted us to extend our observations further. For this purpose sera from 41 patients was collected. One more case of alloalbuminaemia of the fast type was detected. However, in contrast to our first series of patients, quite high incidence (12.2%) of transferrin variants was recorded in this sample. Family studies were conducted on all the patients with protein anomalies.
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PMID:Serum protein polymorphisms in breast cancer. 654 52

Collagen, a major component of the extracellular matrix, is important in maintaining the in vivo characteristics of epidermal cells in vitro. In the present study, the morphological and proliferative characteristics of two human mammary epithelial cell lines (T-47D and MCF-7) cultured in cowhide collagen (Vitrogen 100) were studied. When grown in collagen, the tumor cells displayed a spherical shape and formed multilayered, tumorlike aggregates; desmosomes were observed between cells. In contrast, both cell lines grew as monolayers on plastic substratum; cells were characteristically flat and polygonal. When grown in collagen matrix, the human breast cancer cells became more dependent on serum for growth: cells proliferated in the presence of 10% fetal bovine serum (FBS) but failed to grow in 1% serum. On the other hand, these cells proliferated rapidly in 1% serum when they were grown on plastic. Even in 10% serum the doubling time of cells cultured in collagen was longer than that of cells maintained on plastic. In addition, cells cultured in collagen proliferated rapidly in a serum-free medium containing insulin, epidermal growth factor (EGF), estrogen, and transferrin. The collagen gel system may be useful for characterizing physiologically important trophic factors that regulate the proliferation and other functions of human breast tumor cells.
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PMID:Morphological and proliferative characteristics of human breast tumor cells cultured on plastic and in collagen matrix. 674 55

From early 1972 to the end of 1976, the profiles of several serum protein were used to monitor disease stage and prognosis of 207 patients with breast cancer. Six of these proteins, alpha 1-antitrypsin (alpha-AT), alpha 2-ceruloplasmin (Cp), beta 1-transferrin, IgA, C4, and C5, were significantly elevated in these cancer patients and were used as biologic markers in a multiparametric study. Among these breast cancer patients, 72% had at least two of these protein levels elevated, of which alpha-AT (55%), C5 (38%), and IgA (36%) levels were most commonly raised. The number of elevated proteins was parallel to disease progression as 61% (Group 1) and 74% (Group 2) of the patients with operable breast cancer and 90% of patients with metastatic disease showed an elevation of two or more of these nonspecific proteins. There was also a positive correlation between the number of elevated proteins and prognosis; of the 26 patients who died during the five-year follow-up, only four (15%) had no more than one protein level elevated, and 22 (85%) had two or more protein levels elevated. On the other hand, when considered as a group, patients with no or only one protein level elevated had a better prognosis than patients with two or more levels elevated (P less than 0.03). This multiparametric study tends to indicate that the high level of these serum proteins, reflecting an abnormal biochemical profile, provides valuable information that relates to the stage of the disease and patients' prognosis. Results also suggest that these proteins may aid in differentiating the group with high recurrent risks from that with a more favorable prognosis for a given clinical and pathologic stage, illustrating their importance as biologic markers in breast cancer.
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PMID:Biologic markers and breast cancer: a multiparametric study--1. Increased serum protein levels. 697 5

Lowered serum concentrations of albumin, IgG, IgM, and transferrin have been identified preoperatively in a population of otherwise healthy white women over age 40 with early stage breast cancer. Definition of low values for each of the four serum proteins has been arrived at via comparison with age-matched controls, consisting of disease-free women and women with benign breast lesions. Thus defined, low values for the individual serum proteins have been found to occur in malignant/control study subjects at the following frequencies: albumin 68%/4.7% (P less than 0.0001); IgG 56%/21% (P less than 0.02); IgM 59%/19% (P less than 0.001), and transferrin 50%/4.7% (P less than 0.0001). Among the relevant historical and pathologic data evaluated in addition to the presence or absence of malignancy, only age has been found significant in influencing serum protein concentrations, and this has been taken into account in analyzing results. Forty-four percent of study subjects subsequently found to have breast cancer have low concentrations of at least three of the four discriminant proteins simultaneously in the pre-operative sample. None of the controls have these findings. Twenty-nine percent of women with a malignant breast lesion and 9% of controls have simultaneously low concentrations of two of the four discriminant proteins. Using these measurements of serum proteins it thus becomes possible to assign risk of malignancy when a woman is found to have a breast mass.
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PMID:Serum protein changes in women with early breast cancer. 724 7


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