Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0005940 (
bone disease
)
7,459
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Antisera against purified human
placental alkaline phosphatase
(
PAP
) and crystallized creatine kinase (CK) isoenzyme from human skeletal muscle (MM) were raised in rabbits. The
PAP
antiserum was shown by radial immunodiffusion not to react with purified alkaline phosphatases from human liver and intestine, nor with the alkaline phosphatase in sera from patients with osteoblastic
bone disease
. CK antiserum also demonstrated no cross-reaction and was precipitated quantitatively by its homologous antisera. A "rocket" electroimmunoassay for
PAP
and CK is described. The method is simple, reproducible and uses small volumes of antiserum. The isoenzyme patterns were compared with those developed by several electrophoretic methods. These techniques share with other immunoassay the advantages of specificity for the antigen and enhance the quantitation of isoenzyme assays.
...
PMID:Immunoassay of enzymes. 40 31
