Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0005940 (bone disease)
 7,459 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this method for quantifying 4-hydroxyproline in human urine, 50 microL of urine is hydrolyzed, derivatized with phenylisothiocyanate (PITC), and then quantified by reversed-phase HPLC with ultraviolet detection. The detection limit in urine is 373 pg of hydroxyproline per 50-microL injection. The total CVs for high- and low-concentration pools are 5.3% and 3.9%, respectively (10 runs in 10 days). The standard curve of the assay is linear over a range of 0 to 22 nmol per injection. We estimated the normal range for hydroxyproline excretion in men on an unrestricted diet to be 123-308 mumol/24 h. We also report hydroxyproline concentrations in patients with metastatic bone disease and cirrhosis of the liver.
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PMID:Liquid-chromatographic determination of total hydroxyproline in urine. 340 56

A brief description of the structure of collagen and the special features of each type of collagen, followed by a summary of its metabolism, serve as an introduction to the major pathological processes involving the collagen molecule; mutations, inflammatory syndrome bone diseases, basement membrane diseases. The measurement of hydroxyproline isomers in urine is the basic biochemical test for diseases involving the collagen molecule. 4-hydroxyproline is increased in bone disease of Paget and in cancerous metastases of bone, 3-hydroxyproline is increased in case of polycystic kidney. It is possible, but not very useful, to measure serum 4-hydroxyproline. Of more interest, is the radio-immunological measurement of the N-terminal extension of the type III procollagen molecule, which can differentiate ordinary hepatitis from cirrhogenous hepatitis. New and useful information can be gained from tissue biopsy: the proportion of the various types of collagen, the measurement of their hydroxylation and the activity of the enzymes acting on their biosynthesis.
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PMID:[Techniques for studying collagen in medical practice]. 676 Jul 52

Using molecular sieve chromatography on Bio-Gel P-2 and then on Bio-Gel P-30, hydroxyproline-containing urinary polypeptides (molecular weight greater than 1500 daltons) were separated into eight fractions. The three main fractions were separated further on phosphocellulose giving seven, nineteen and twelve peaks, respectively, each containing 4-hydroxyproline. Hypotheses about the origin of certain polypeptides are proposed, which take into account the sequence of type I collagen. Among these 38 polypeptides only one shows a quantitative variation in Paget's bone disease and was thus purified. It consists of equal amounts of glycine, proline and 4-hydroxyproline. This particular polypeptide may originate from the N-terminal propeptide of type I collagen.
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PMID:Polymorphism of urinary 4-hydroxyproline-containing polypeptides. 729 59