Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0005684 (
bladder cancer
)
16,431
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recently, microRNA-1247 (miR-1247) has been reported to function as tumour suppressor in several cancer types, including pancreatic cancer, hepatocellular cancer and lung cancer. However, the biological function of miR-1247 in
bladder cancer
and the underlying mechanisms have remained largely uncovered. In this study, the expression of miR-1247 was significantly downregulated, while
RAB36
protein was remarkably upregulated in
bladder cancer
tissues and cell lines compared with that in paired adjacent normal tissues or normal cell line (SU-HUC-1). The function of miR-1247 and
RAB36
in the cell viability, proliferation and invasion of
bladder cancer
cells (T24 and J82) was assessed by CCK-8, colony formation and Transwell assay, respectively. Gain of function studies showed that upregulation of miR-1247 significantly inhibited cell proliferation and invasion capacity of
bladder cancer
cells. Consistently, downregulation of
RAB36
mimicked the suppressive effects of miR-1247 overexpression in
bladder cancer
cells. Importantly, miR-1247 was confirmed to target the 30untranslated region (UTR) of
RAB36
and downregulated its expression using luciferase reporter assay and Western blot assays. In conclusion, these results provide the first clues regarding the role of miR-1247 might be a potential therapeutic agent and diagnostic marker of
bladder cancer
by inhibiting
RAB36
expression.
...
PMID:Inhibition of miR-1247 on cell proliferation and invasion in bladder cancer through its downstream target of RAB36. 2987 24
