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Query: UMLS:C0005684 (bladder cancer)
 16,431 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We describe a modification of the Yang Pros-check radioimmunoassay for prostate-specific antigen (PSA) that increases the analytical sensitivity of the assay approximately threefold (from a working range of 0.3-50 to 0.1-1.2 micrograms/L). It can detect PSA added to zero-concentration diluent (bovine serum albumin solution) at 0.10 microgram/L or added to zero-concentration control female serum at 0.20 microgram/L (P less than 0.05). In 26 patients tested after cystoprostatectomy for bladder cancer (who had normal prostates without cancer on histologic examination), PSA values by this ultrasensitive assay were all less than 0.10 microgram/L. Therefore, we propose this value as the upper limit of the 95% reference interval. In a retrospective study of two patients who developed recurrent prostate cancer, serum PSA values increased above the 0.1 microgram/L detection limit 175 and 581 days before increasing above the 0.3 microgram/L detection limit of the standard Yang assay. This ultrasensitive radioimmunoassay of PSA should prove more useful than current methods for detecting early recurrence of prostate cancer.
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PMID:Ultrasensitive radioimmunoassay of prostate-specific antigen. 137 91

The commonly employed methods for the early detection of urologic cancers remain the traditional techniques of a carefully performed history and physical examination. Newer developments include, among others, prostate-specific antigen and monoclonal antibodies in prostate cancer, flow cytometry in bladder cancer, computerized axial tomography (CAT) scanning in renal cancer, and ultrasound in testicular cancer. These and other new diagnostic techniques, with further testing and wider use, will hopefully permit the earlier diagnosis of genitourinary cancer.
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PMID:Methods of early diagnosis in genitourinary cancer. 329 92

Serum prostate-specific antigen (PA), a new tumor marker of prostate cancer, was evaluated with an eznyme immunoassay (EIA) in various urologic cancer patients and benign prostatic diseases in Japanese. Sera of prostate cancer patients before treatment (n = 27) revealed a range of PA concentrations from 0.12-23 ng/ml with a mean (+/- SD) of 5.78 +/- 6.85 ng/ml, while that of patients with benign prostatic hypertrophy (BPH) (n = 27) showed from less than 0.10 to 2.6 ng/ml with 0.84 +/- 0.81 ng/ml (mean +/- SD). The mean serum PA levels in nonprostatic cancers were calculated as follows: bladder cancer (n - 21), 0.77 +/- 0.55 ng/ml; renal pelvis or ureteral cancer (n = 6), 0.46 +/- 0.47 ng/ml; renal adenocarcinoma (n equal to 6), 1.07 +/- 0.77 ng/ml; other urologic cancers (n = 6), 0.55 +/- 0.52 ng/ml. Serum PA ranged from less than 0.10 to 1.1 ng/ml in patients with prostatitis (n = 5). A significant statistical difference in serum PA levels between prostate cancer and other groups was recognized. These results suggested that an elevation of serum PA value was highly specific to prostate cancer in urological malignancies. The evaluation of serum PA may be of great value in the detection of prostate cancer.
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PMID:Evaluation of serum prostate-specific antigen in urologic cancers. 619 84

Several serum tumor markers have been studied in different types of epithelial cell-associated cancer. The application of these markers in clinical oncologic practice is hampered by insufficient sensitivity and specificity in most primary tumors. It is important to define which markers contribute to patient management. Prostate-specific antigen can be used in prostate cancer patients for screening and monitoring advanced disease. Cancer antigen 125 is primarily used for the monitoring of combined chemotherapy in ovarian cancer patients. In breast cancer patients preoperative levels of cancer antigen 15-3, carcinoembryonic antigen, and tissue polypeptide antigen specific do not contribute in prognosis, but changes in the levels of these markers predict the clinical outcome in the treatment of advanced disease better than UICC criteria. Carcinoembryonic antigen and tissue polypeptide antigen specific can detect recurrence in colorectal cancer patients earlier than imaging methods. Tissue polypeptide antigen specific is sensitive in measuring response to combined chemotherapy in advanced gastrointestinal cancer. In bladder cancer, urine levels of cytokeratins are sensitive in indicating advanced disease. In small-cell lung cancer neuron-specific enolase is a good indicator of chemotherapy response. In non-small-cell lung cancers, cytokeratins may also predict response in chemotherapy treatment. Clinical application of tumor markers in the correct circumstances can omit more invasive and costly procedures and will contribute to better patient care.
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PMID:How to integrate serum tumor markers into clinical oncologic practice. 874 5

We have developed a highly sensitive method to detect pelvic lymph node metastasis using the reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific for prostate-specific antigen (PSA) gene. Fine needle aspiration biopsy (FNAB) of pelvic lymph nodes was performed in 24 patients with prostate cancer. Each aspirated sample (0.05-0.1 ml) was divided into 2 parts; one for RNA extraction and RT-PCR to detect the fragment of PSA mRNA, and the other to smear on a slide glass for conventional cytology. The PSA gene was detected by RT-PCR in 11 FNAB samples which included not only all 6 cytologically positive and 2 cytologically class III cases but also 3 of 16 cytologically negative cases. The PSA gene was not detected by RT-PCR of FNAB samples in any of the 20 cases of bladder cancer. Thus RT-PCR for detection of the PSA gene in FNAB samples may be useful as a new diagnostic technique for detection of early lymph node metastasis in prostate cancer and an additional tool for cytological diagnosis of prostate cancer.
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PMID:[Genetic diagnosis of pelvic lymph node metastasis using fine needle aspiration samples in prostate cancer]. 895 75

We have developed a highly sensitive method to detect pelvic lymph node(LN) metastasis using reverse transcriptase-polymerase chain reaction(RT-PCR) with the primers specific for prostate-specific antigen(PSA) gene in combination with the fine needle aspiration biopsy(FNAB). The specimens were obtained from pelvic LN from 15 prostate cancer patients and 15 bladder cancer patients. The aspirated samples (0.05 approximately 0.1 ml) were used for detecting the fragment of PSA mRNA by RT-PCR and Southern blot analysis, and the rest of samples were submitted to conventional cytology. Expression of PSA gene was detected in 9 cases of FNAB samples including all 5 cytologically positive and further more 2 cytologically class III cases, and 2 of 8 cytologically negative cases. RT-PCR of FNAB samples from all cases of bladder cancer were negative for the detection of PSA gene. The sensitivity of PSA gene by RT-PCR was very high and could detect 10 degrees cancer cell. In conclusion, our study suggested that RT-PCR for detection of PSA gene in FNAB samples might become a new diagnostic tool for detection of small foci of prostatic cancer metastasis in LN and combination use of RT-PCR and cytology could greatly contribute to accuracy in diagnosis.
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PMID:[Genetic diagnosis of pelvic lymph node metastasis in prostate cancer using aspiration biopsy samples]. 899 Sep 38

BACKGROUND: The incidence of bladder and prostate cancer continues to rise, with little accompanying improvement in management strategies. Opportunities exist for testing various types of chemopreventive interventions. METHODS: The authors review the biology of progression to invasive disease for cancers of the bladder and the prostate and identify intermediate disease and surrogate endpoint markers. Candidate interventions and initial clinical trial results are described. RESULTS: Markers of cellular proliferation and differentiation, as well as antigens such as Le(x), M344, DD23, and bladder tumor antigen, are promising for bladder cancer. Testing with prostate-specific antigen and prostate-specific membrane antigen is promising for prostate cancer. Several prevention intervention trials are in progress for both cancers. CONCLUSIONS: Vitamins, polyamine synthesis inhibitors, and oltipraz are undergoing clinical tests for chemopreventive effects in bladder cancer, and a large trial of finasteride to prevent prostate cancer is completing accrual. Results from these studies will direct future research.
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PMID:Chemoprevention of Bladder and Prostate Carcinoma. 1076 11

Appreciation of the clinical utility of the protein product of kallikrein gene 3, prostate-specific antigen (PSA), has resulted in earlier diagnosis of prostate cancer and an associated increase in the number of radical prostatectomies performed. A consequence of this change in surgical practice has been an improved understanding of sphincter anatomy and methods for sphincter preservation, which in turn have led to enhanced popularity for orthotopic urinary diversion for invasive bladder cancer. In this paper, molecular and basic science research being undertaken in an to attempt to overcome problems and limitations of the PSA/transrectal ultrasonographic biopsy approach to diagnosis are discussed. Also detailed are (1) the development of a bladder acellular matrix graft to serve as an "off the shelf" scaffold on which urothelium regenerates, (2) attempts to create a simpler, more durable bowel continence mechanism, and (3) a novel experimental technique for renal preservation based on considering the urine-producing and urine-directing roles of the upper tract as surgically separable entities. These research endeavors serve to illustrate how developments at the molecular and basic science levels promise to lead to further reconstructive surgical approaches when translating new developments into patient benefits during the year 2000 and beyond.
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PMID:Molecular and reconstructive urology: a coming together. 1107 52

The further course of prostate cancer (PC) after radical prostatectomy (RPX) is decisively influenced by the local tumor stage. Although it is now possible to assess the risk of local recurrence from the histopathology, precise predictions cannot be made. A more accurate evaluation would be desirable, mainly for early planning of adjuvant therapy. We assessed the detection of telomerase activity using the Telomeric Repeat Amplification Protocol in surgical margins compared to RT-PCR-supported prostate-specific antigen (PSA) mRNA detection and conventional histopathological examination. We examined 95 patients with local PC during RPX and 16 patients with muscle-invasive transitional bladder cancer who underwent radical cystectomy. After RPX or RCX biopsies were obtained from 4 or 3 defined areas of the prostatic fossa and processed by TRAP assay for telomerase activity and by RT-PCR for PSA using a standard protocol. Five of 48 patients (10.4%) with organ-confined prostate cancer (pT2) and 7 of 47 patients (14.9%) with locally advanced PC (>pT2) had positive detection of telomerase activity in at least one positive specimen. In contrast to RT-PCR for PSA mRNA and histological findings for organ-confined and locally advanced disease, detection of telomerase activity yielded no statistically significant correlation to clinical parameters. Only PC-patients with positive histopathological margins had a positive correlation between detection of telomerase activity and high Gleason scores (r=0.65, p=0.022). Based on the results obtained and the current state of knowledge, measurement of telomerase activity must be considered less sensitive than RT-PCR for PSA mRNA in surgical margins, although tumor specificity should theoretically be higher. It is not even sure at the present time whether a combination of both methods offers any recognizable advantage over PSA mRNA detection alone. The value of the results obtained in this study will have to be assessed in a further follow-up to determine whether patients with positive molecular detection have an increased risk of local recurrence.
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PMID:Molecular staging of pelvic surgical margins after radical prostatectomy: comparison of RT-PCR for prostate-specific antigen and telomerase activity. 1195 25

The differential diagnosis between poorly differentiated prostate adenocarcinoma (PAC) involving the bladder and high-grade urothelial bladder cancer (UC) with prostate extension can be very challenging. The aim of this study is to evaluate the use of a panel of antibodies to distinguish the poorly differentiated forms of these two tumors. We evaluated a series of 40 PAC cases (Gleason's grade >/= 8) and 45 (G3) UC cases obtained from transurethral endoscopic resection material. Immunohistochemical analysis was performed using the following antibodies: prostate acid phosphatase (PAP), prostate-specific antigen (PSA), uroplakin III (UP), thrombomodulin (TM), cytokeratin (CK) 7, and CK20. PAC expressed PSA and PAP in 34 and 38 cases, respectively. The sensitivity and specificity of expressing at least 1 marker (PSA+ or PAP+) is 95% and 100%, respectively. All UC cases were negative for both markers. UC expressed UP and TM in 27 and 22 cases, respectively. In addition, 36 of 45 cases stained positively for at least 1 marker (UP + or TM +) with specificity and sensitivity of 80% and 100%, respectively. All cases of PAC were negative for both markers. Twenty-eight UC cases were CK7+/CK20 +, and 4 PAC cases stained positively for both markers. On the other hand, 29 PAC cases and 4 UC cases were CK7-/CK20-. We concluded that PSA, PAP, UP, and TM are very useful markers in differentiating poorly differentiated UC from PAC. Finally, when all 4 markers (PAP, PSA, UP, and TM) were negative, CK7 and CK20 appeared of no major use in making the differential diagnosis.
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PMID:Immunohistochemical profile of high-grade urothelial bladder carcinoma and prostate adenocarcinoma. 1245 20


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