Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0004153 (
atherosclerosis
)
77,401
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Oxygen toxicity and related free radical reactions are implicated in numerous pathophysiological conditions, like
atherosclerosis
, inflammation, gastric ulceration, neuronal degeneration, tumour promotion. The flowers of Matricaria chamomilla, Asteraceae, have been used therapeutically for conditions in which oxidative stress is supposed to be implicated. We considered interesting to investigate the effect of
Chamazulene
, the active substance of chamomile, on free radical processes. Membrane lipid peroxidation was induced by Fe2+/ascorbate and assessed as the 2-thiobarbituric acid reactive material. The hydroxyl radical scavenging activity was studied as the competition of
Chamazulene
with DMSO for HO. generated by Fe3+/ascorbate. Finally, the interaction of
Chamazulene
with the N-centered stable free radical DPPH was estimated photometrically (517 nm). It was found that
Chamazulene
inhibited lipid peroxidation in a concentration and time dependent manner presenting an IC50 of 18 microM after 45 min incubation. It could also inhibit the autoxidation of DMSO (33 mM) by 76% at 25 mM, and had a weak capacity to interact with DPPH. In conclusion,
Chamazulene
presents interesting properties concerning radical processes.
...
PMID:Investigation of the effect of chamazulene on lipid peroxidation and free radical processes. 882 32
Endothelial cells surround the lumen of blood vessels and modulate many physiological processes, including vascular tone, blood fluidity, inflammation, immunity and neovascularization. Many pathological conditions, including hyperglycemia, may alter endothelial function through oxidative stress, leading to impaired nitric oxide bioavailability and to the onset of an inflammatory state. As widely shown in the last decade, dietary intervention could represent a good strategy to control endothelial dysfunction and
atherosclerosis
. In particular, extensive research in the field of antioxidant natural derivatives has been conducted. In this study, we evaluated the capability of
Chamazulene
(Cham), an azulene compound from chamomile essential oil, to attenuate ROS levels in bovine aortic endothelial cells (BAECs) stressed with either high glucose or H
2
O
2
. Cell viability at different concentrations of Cham was evaluated through the WST-1 assay, while ROS production acutely induced by High Glucose (HG, 4.5 g/L) treatment or H
2
O
2
(0.5 mM) for 3 h, was quantified with 2'-7'-Dichlorofluorescein diacetate (DCFH-DA) probe using confocal microscopy and flow cytometry. Our results showed a reduction in ROS produced after simultaneous treatment with High Glucose or H
2
O
2
and Cham, thus suggesting an
in vitro
antioxidant activity of the compound. On the whole, this study shows for the first time the potential role of Cham as a scavenging molecule, suggesting its possible use to prevent the rise of endothelial ROS levels and the consequent vascular damage.
...
PMID:Chamazulene Attenuates ROS Levels in Bovine Aortic Endothelial Cells Exposed to High Glucose Concentrations and Hydrogen Peroxide. 2961 34
