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Query: UMLS:C0004153 (atherosclerosis)
 77,401 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using the histological staining methods of Weigert and of Masson on primary cultures of rat aortic media cells, we obtained additional proofs of the smooth muscle cell's ability to secrete collagen and elastin in vitro: the percentage of positive flasks with aorta rings was the same throughout the follow-up, but increased gradually for the new tissue growing around the rings.
Atherosclerosis 1976 Oct
PMID:Histological arguments for collagen and elastin synthesis by primary cultures of rat aortic media cells. 6 79

Lysyl oxidase is the copper-dependent enzyme responsible for the normal cross-linking of both collagen and elastin which is necessary for their functional integrity. There is now strong evidence that this enzyme is vitamin-B6-dependent. The earliest visible lesion of atherosclerosis, commonly found in human neonatal coronary arteries and probably indicative of the location of future atherosclerotic plaques, is a focal splitting of the internal elastic lamina, the cause of which has hitherto remained unexplained. It is suggested that this lesion is the result of imperfect cross-linking of arterial elastin as well as collagen, and is caused by a maternal deficiency of vitamin B6 which is commonly found in pregnancy and which could thus impair the function of lysyl oxidase. Prophylactic supplementation of maternal diet with adequate vitamin B6 is therefore suggested.
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PMID:The aetiological role of maternal vitamin-B6 deficiency in the development of atherosclerosis. 6 31

Two neutral proteases have been isolated from aortas and human breast tumors. The aortic elastase-like enzyme has been further purified. The details of this purification procedure will be given and some of the properties of the purified enzyme (susceptibility to various kinds of substrates, degree of inhibition of serum inhibitors, alpha 1-antitrypsin and alpha 2-macroglobulin). This elastinolytic activity of the aorta increased with age and with the degree of atherosclerosis. Both parameters seem to act independently and in a cumulative fashion. Elastinolytic activity has been demonstrated in extracts of human breast carcinomas and is exponentially related to the age of the patient. There exists a parallel neosynthesis of elastin which increased also with the age of the patient. Some characteristics of the polymeric elastin isolated from the tumor tissue will be given. The possible role of this neutral protease present in human aortas and human breast carcinomas will be discussed.
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PMID:Elastase-like enzymes in aortas and human breast carcinomas: quantitative variations with age and pathology. 6 61

Aortic pulse wave velocity was determined in Macaca fascicularis monkeys fed either atherogenic or control diets for 36 months. The foot-to-foot velocity and apparent phase velocities of the second through seventh Fourier harmonics at a given diastolic pressure in the atherosclerotic monkeys were 1.5 to 2.0 times the values for the control animals. More than 80% of the aortic intimal surface of the atherosclerotic monkeys was covered with fibrous or fatty plaque, which approximately doubled wall thickness and wall thickness to radius ratio. Angiochemical evaluations showed no difference in collagen or elastin concentration (as a fraction of lipid and mineral-free dried aorta), but the atherosclerotic aortas were 1.5 to 2.0 times that of control in collagen and elastin content (defined as the absolute quantity beneath a square centimeter of intimal surface). Total cholesterol and calcium concentrations in the atherosclerotic aortas were more than 10 times the values for the control aortas. The static circumferential distensibility of the excised atherosclerotic aortas was significantly less than control, but there was no difference in incremental (Young's) modulus of elasticity. The in vitro pressure-strain elastic modulus of the atherosclerotic aortas was more than twice that of control, which was predicted from the enhanced wave velocity. The significantly increased stiffness of the atherosclerotic arteries appeared to be due mainly to the increased wall thickness caused by the atherosclerotic plaques rather than to material changes described by Young's modulus. Extensive medial damage, however, also was present and could have had a major influence on stiffness. Atherosclerosis therefore can result in increased aortic stiffening, detectable by pulse wave velocity, even if there is no change in the overall Young's modulus of elasticity.
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PMID:Aortic pulse wave velocity, elasticity, and composition in a nonhuman primate model of atherosclerosis. 9 6

The effects of the anticalcifying drug, ethane-hydroxydiphosphonate (EHDP) and the inhibitors of collagen biosynthesis, colchicine, penicillamine and azetidine were studied in the rabbit with pre-established atherosclerosis. The drugs were administered with a cholesterol-free diet (regression diet) for 8 weeks following the induction of atherosclerosis by feeding a hypercholesterolemic diet containing 2% cholesterol and 8% peanut oil for 8 weeks. The extent and severity of aortic atherosclerosis, as revealed by the morphological and biochemical findings, increased significantly during the regression period. In rabbits treated with EHDP (5 mg/kg/day) the aorta had fewer gross lesions and contained significantly less cholesterol, collagen and elastin than did the aorta of the rabbits fed the regression diet alone. These changes were associated with a significant reduction in aortic calcium caused by EHDP. The aortic content of cholesterol, collagen and elastin in the EHDP-treated rabbits, although less than that of the rabbits fed the regression diet alone, was about the same as that of the rabbits fed a high cholesterol diet for 8 weeks. Both colchicine (0.2 mg/kg/day) and penicillamine (100 mg/kg/day) had a selective action on the induced plaques in that they suppressed the fibrous proliferation in the lesions without preventing lipid and calcium accumulation in the lesions. Neither colchicine nor penicillamine reduced the extent of aortic atherosclerosis as determined by gross examination of the vessel. Azetidine had no significant effect on the pre-established atherosclerotic lesions. The lipid, fibrous protein and calcium content of the aorta of the azetidine-treated animals was not significantly different from that of the untreated animals. The biochemical findings in the aorta were consistent with the microscopic changes.
Atherosclerosis 1979 May
PMID:Effects of anticalcifying and antifibrobrotic drugs on pre-established atherosclerosis in the rabbit. 11 83

Electron microscopy of ruthenium red stained bovine aorta before and after chondroitinase digestion demonstrates proteoglycans on and between collagen fibrils. The collagen-associated proteoglycans include a proteoglycan previously purified from this tissue as demonstrated by immunocytochemistry and are extractable with high molar guanidine HC1. In loci rich in proteoglycans such as areas of turbulent flow in calves, more proteoglycan can be demonstrated morphologically, and these molecules also coat elastin.
Atherosclerosis
PMID:The ground substance of the arterial wall. Part 2. Electron-microscopic studies. 13 92

Mitral valve, coronary arteries, cartilage, and liver were studied by light and electron microscopy in a 15 year old boy with Morquio's syndrome, a genetic mucopolysaccharidosis, in which a deficiency of lysosomal hexosamine sulfatase is associated with accumulations of keratan sulfate in various organs. Coronary artery intimal sclerosis was a prominent feature of this disorder. Ultrastructural examination revealed numerous intimal smooth muscle cells containing storage vacuoles consistent with lysosomes. This was associated with marked interstitial deposition of collagen, elastin, and basement membrane material. Recent studies of human and experimental atherosclerosis have demonstrated the accumulation of cholesterol within vascular smooth muscle cell lysosomes. Intralysosomal accumulation of substrates other than cholesterol is also associated with vascular intimal sclerosis in genetic lysosomal disorders such as Fabry's disease and Hurler's syndrome. Lysosomal storage of undegraded substrate may be an important pathogenetic mechanism in the development of sclerotic vascular lesions.
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PMID:Coronary intimal sclerosis in Morquio's syndrome. 15 Jun 85

Elastin preparations were isolated from human thoracic aorta, from atherosclerotic and from grossly normal regions. A relatively mild procedure was used to avoid hot alkaline extraction and autoclaving. The elastase digest of the aortic elastin was chromatographed on a Sephadex G-100 column and separated into two fractions: A (larger molecular weight) and B (smaller molecular weight). The ratio of fraction A to total aortic elastin increased with age and the development of the atherosclerosis. Amino acid and sugar analyses showed that fraction A consistently contained more polar amino acids, hexose, hexosamine and L-fucose, and less sialic acid, in comparison with fraction B. Part of the elastin preparation was incubated with human low-density lipoprotein; a considerable amount of lipid, especially cholesterol, was transferred from the lipoprotein to the elastin. Estimation of protein and cholesterol in fractions A and B of the elastase hydrolyzate of incubated elastin showed that most of the cholesterol taken up by elastin had been in fraction A. The increased proportion of fraction A in aortic elastin derived from plaque areas appeared responsible for the marked lipid-binding capacity of plaque elastin.
Atherosclerosis 1977 Oct
PMID:Elastin sub-fraction as binding site for lipids. 19 4

Atherosclerosis results from a precise sequence of endothelial interaction with platelets or thrombocytes and mononuclear cells, uptake of specific lipoproteins, cholesteryl ester removal, production of collagen, elastin ad proteoglycans by activated smooth muscle cells. Thus, both the filtration hypothesis and the thrombogenic hypothesis for the pathogenesis of atherosclerosis appear to be correct.
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PMID:Smooth muscle cells of intimal cushions and the localization of atherosclerotic lesions. 20 Sep 60

An isotopic assay of elastolytic activity is performed; the iodine-125 ions retained inside the elastin framework were removed by appropriate treatment. This acute substrate enables us to study the role of trypsin on elastase activity and facilitates the study of elastase role in atherosclerosis.
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PMID:[Improvements in the technical conditions for the evaluation of an elastolytic activity. The role of tryspin as an activator (author's transl)]. 23 67


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