Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0004135 (
ATM
)
13,001
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The mammalian alveolar epithelium is composed of alveolar type I (
AT1
) and alveolar type II (AT2) cells that together coordinate tissue function. We used a heterocellular culture model of
AT1
and AT2 cells to determine pathways for intercellular signaling between these two phenotypes.
Gap junction protein
(connexin) profiles of
AT1
and AT2 cells in heterocellular cultures were similar to those seen in rat lung alveolar sections. Dye coupling studies revealed functional gap junctions between and among each cell phenotype. Localized mechanical stimulation resulted in propagated changes of intracellular Ca2+ to
AT1
or AT2 cells independent of the stimulated cell phenotype. Ca2+ communication that originated after
AT1
cell stimulation was inhibited by gap junction blockers, but not by an inhibitor of extracellular nucleotide signaling (apyrase). Conversely, Ca2+ communication after stimulation of AT2 cells was not significantly reduced by gap junction inhibitors. However, apyrase significantly reduced Ca2+ communication from AT2 to
AT1
cells, but not from AT2 to AT2 cells. In conclusion,
AT1
and AT2 cells have unique connexin profiles that allow for functional coupling and distinct intercellular pathways for coordination of Ca2+ signaling.
...
PMID:Cell-cell communication in heterocellular cultures of alveolar epithelial cells. 1274 60
