Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0004135 (
ATM
)
13,001
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Molecular and functional characterization of alveolar epithelial type I (
AT1
) cells has been challenging due to difficulty in isolating sufficient numbers of viable cells. Here we performed single-cell RNA-sequencing (scRNA-seq) of tdTomato
+
cells from lungs of
AT1
cell-specific Aqp5-Cre-IRES-DsRed (ACID);R26tdTomato reporter mice. Following enzymatic digestion, CD31
-
CD45
-
E-cadherin
+
tdTomato
+
cells were subjected to fluorescence-activated cell sorting (FACS) followed by scRNA-seq. Cell identity was confirmed by immunofluorescence using cell type-specific antibodies. After quality control, 92 cells were analyzed. Most cells expressed 'conventional'
AT1
cell markers (
Aqp5
,
Pdpn
,
Hopx
,
Ager
), with heterogeneous expression within this population. The remaining cells expressed AT2, club, basal or ciliated cell markers. Integration with public datasets identified three robust
AT1
cell- and lung-enriched genes,
Ager
,
Rtkn2
and
Gprc5a
, that were conserved across species. GPRC5A co-localized with HOPX and was not expressed in AT2 or airway cells in mouse, rat and human lung. GPRC5A co-localized with AQP5 but not pro-
SPC
or CC10 in mouse lung epithelial cell cytospins. We enriched mouse
AT1
cells to perform molecular phenotyping using scRNA-seq. Further characterization of putative
AT1
cell-enriched genes revealed GPRC5A as a conserved
AT1
cell surface marker that may be useful for
AT1
cell isolation.
...
PMID:Integrated Single-Cell RNA-Sequencing Analysis of Aquaporin 5-Expressing Mouse Lung Epithelial Cells Identifies GPRC5A as a Novel Validated Type I Cell Surface Marker. 3318 67
