Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0003129 (Anoxia)
 551 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The cell death and gene expression in neonatal cardiomyocyte cultures were investigated in a late postconditioning model. The primary cultures were subjected to a 30min of anoxia followed by 60min or 24h of reoxygenation. Postconditioning was carried out in three cycles of 1min reoxygenations followed by 1min anoxia, respectively. After 24h of reperfusion the percentages of living, necrotic, and apoptotic cells were determined by staining with bis-benzimide and propidium iodide. Anoxia-reoxygenation significantly increased the necrotic and apoptotic cells both at its first and second episodes. Postconditioning in remote period did not protect the cells from the second anoxia. Postconditioning decreased the anoxia-reoxygenation-induced increase of HSP70 and HSP90 mRNA expression. We observed a decrease of HIF-3alpha gene expression in remote postconditioning. The FRAP gene expression was leveled to control value. Thus, the changes of mRNA gene expression did not show cytoprotection of cardiomyocytes in remote postconditioning model.
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PMID:Effects of late postconditioning on gene expression and cell death in neonatal rat cardiomyocyte cultures. 1928 60

The cells death and genes expression in neonatal cardiomyocytes culture at two anoxia-reoxygenation modeling were investigated. The primary culture of neonatal cardiomyocytes was undergone 30 min of anoxia followed by 24 h (A-R1) and the second anoxia-reoxygenation--30 min and 60 min respectively (A-R2). The percentages of living, necrotic, apoptotic and autophagic cells were determined by staining with bis-benzimide, propidium iodide and monodansylcadaverine. Anoxia-reoxygenation significantly influenced the ratio of living, necrotic, apoptotic and autophagic cells both at its first A-RI and second A-R2 episodes. It was shown that the main mechanism of cell death after the both periods of anoxia-reoxygenation is necrosis. The changes of mRNA levels of genes of heat shock proteins HSP70 and HSP90, antiapoptotic protein Bcl2 and key regulator of autophagy FRAP in cardiomyocytes culture were established. The data obtained allow to make suggestion that in 24 h after the first episode of anoxia-reoxygenation A-R1 the overexpression of heat shock proteins starts the cascade of reactions that causes the necrotic cell death prevalent and the blocking of apoptotic program at second anoxia-reoxygenation A-R2.
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PMID:[Second anoxia-reoxygenation does not cause the apoptotic cell death of cultured neonatal cardiomyocytes]. 1944 11