Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0002871 (
anemia
)
52,094
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Haemonchus contortus
is recognized as one of the important health problems in small ruminants, leading to reduced production and economic loss for farmers worldwide. Prepatent diagnosis of
H. contortus
infection is crucial to improve control strategies as this helminth may remove up to one-fifth of total erythrocytes and may cause
anemia
, edema, diarrhea, and ultimately death in young animals. In this study, one of the excretory and secretory products, rHc-
HCA59
, was purified and used as antigen to detect specific antibodies in
H. contortus
infected goats during prepatent stage of infection using indirect enzyme linked immunosorbent assay (ELISA) as screening test. All goats (
n
= 38) were housed indoor, experimentally infected with 8000 infective larvae (L3) of
H. contortus
, and serum samples were collected prior to infection and at 14th day of infection. Immunoblotting was performed to confirm the results of indirect ELISA, evaluate the cross reactivity against rHc-
HCA59
in sera of most common co-infecting parasites and rectify the false negative samples. Furthermore, three different batches of rHc-
HCA59
were produced to evaluate the repeatability of ELISA. No eggs were detected in feces of all goats collected at 7th and 14th day of infection but,
H. contortus
eggs were detected at 21 days post infection in the feces. Indirect ELISA performed in this study showed 87% sensitivity and 100% specificity. The western blot analysis confirmed immunoreactivity in serum samples which scored positive in indirect ELISA and recognized the samples as negative which had OD
450
lower than negative cut-off value in indirect ELISA. Furthermore, all false negative sera (
n
= 5) that had OD
450
value between positive and negative cut-off value in rHc-
HCA59
based ELISA were clearly positive in western blot. Moreover, no cross-reactivity was detected in ELISA and western blotting against rHc-
HCA59
in positive sera of
Toxoplasma gondii, Fasciola hepatica
, and
Trichinella spiralis.
The results of this study concluded that combined use of indirect ELISA and western blotting with rHc-
HCA59
is a potential immunodiagnostic tool for the detection of
H. contortus
infection during prepatent period in goats.
...
PMID:Combined Use of Indirect ELISA and Western Blotting with Recombinant Hepatocellular Carcinoma-Associated Antigen 59 Is a Potential Immunodiagnostic Tool for the Detection of Prepatent
Haemonchus contortus
Infection in Goat. 3141 73
