Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0001511 (Adhesion)
 5,955 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of this paper was to determine the adhesion of two physico-chemically characterized bacterial strains to a surface hydrophilic (CL A, water contact angle 57 degrees) and hydrophobic (CL B, water contact angle 106 degrees) hydrogel contact lens (CL) with and without an adsorbed tear film in a parallel plate flow chamber. Hydrophobicity (by water contact angles), charge (by particulate microelectrophoresis) and elemental composition (by XPS) of the surfaces of seven bacterial strains were characterized, after which two strains were selected for further studies. On CL surfaces, hydrophobicity, elemental composition, and mean surface roughness (by AFM) were determined, as well as the protein composition of tear films adsorbed on these lenses (by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE)). Bacterial cell surfaces were relatively uncharged and water contact angles on lawns of different strains ranged from hydrophobic to hydrophilic. After adsorption of tear film components, N/C elemental surface concentrations increased on CL A and CL B and differences in water contact angles between both lenses reduced to range from 57 degrees (CL A) to 69 degrees (CL B). However, different protein compositions were inferred. The surface roughness of CL A increased from 4 to 13 nm. while it remained 16 nm for CL B. Adhesion of hydrophobic Pseudomonas aeruginosa #3 was more extensive than of hydrophilic Staphylococcus aureus 799, with no differences between both lenses. The hydrophobicity of P. aeruginosa #3 after cell surface damage decreased and its adhesion was reduced on CL A and strongly on CL B. In addition, passage of an air-liquid interface yielded more detachment of S. aureus 799 than of P. aeruginosa #3 from the CL surfaces. In conclusion, the hydrophobicity of CL surfaces dictates the composition of the adsorbed tear film and therewith plays an important role in bacterial adhesion to lenses. Adhesion of hydrophobic P. aeruginosa #3 was more tenacious than of hydrophilic S. aureus 799.
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PMID:Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses. 1170 Jul 93

Although Streptococcus intermedius and Streptococcus mutans are regarded as members of the commensal microflora of the body, S. intermedius is often associated with deep-seated purulent infections, whereas S. mutans is frequently associated with dental caries. In this study, we investigated the roles of the S. mutans and S. intermedius antigen I/II proteins in adhesion and modulation of cell surface characteristics. By using isogenic mutants, we show that the antigen I/II in S. mutans, but not in S. intermedius, was involved in adhesion to a salivary film under flowing conditions, as well as in binding to rat collagen type I. Binding to human fibronectin was a common function associated with the S. mutans and S. intermedius antigen I/II. Adhesion of S. mutans or S. intermedius to human collagen types I or IV was negligible. Hydrophobicity, as measured by water contact angles, and zeta potentials were unaltered in the S. intermedius mutant. The S. mutans isogenic mutants, on the other hand, exhibited more positive zeta potentials at physiological pH values than did the wild type. The results indicate common and species-specific roles for the antigen I/II in mediating the attachment of S. mutans and S. intermedius to host components and in determining cell surface properties.
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PMID:Functional variation of the antigen I/II surface protein in Streptococcus mutans and Streptococcus intermedius. 1174 90

Alternative splicing of the fibronectin gene transcript gives rise to forms that include the EIIIA (or ED-A) segment. EIIIA-containing fibronectins are prominently expressed during embryogenesis and wound healing and appear to mediate changes in cell adhesion and gene expression. Nonetheless, integrins that bind the EIIIA segment have not been identified. We previously mapped the epitope for two function-blocking monoclonal antibodies to the C-C' loop region of the EIIIA segment (Liao, Y.-F., Wieder, K. G., Classen, J. M., and Van De Water, L. (1999) J. Biol. Chem. 274, 17876-17884). The sequence of this epitope ((39)PEDGIHELFP(48)) resembles the sequence within tenascin-C to which the integrin alpha(9)beta(1) binds. We now report that either integrin alpha(9)beta(1) or alpha(4)beta(1) can mediate cell adhesion to the EIIIA segment. Moreover, this interaction is blocked both by epitope-mapped EIIIA antibodies as well as by the respective anti-integrins. Deletion mutants of the EIIIA segment that include the C-C' loop and flanking sequence bind cells expressing either alpha(9)beta(1) or alpha(4)beta(1). Adhesion of alpha(4)beta(1)-containing MOLT-3 cells to the EIIIA segment stimulates phosphorylation of p44/42 MAP kinase. Our observation that two integrins bind the EIIIA segment establishes a novel mechanism by which cell adhesion to fibronectin is regulated by alternative splicing.
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PMID:The EIIIA segment of fibronectin is a ligand for integrins alpha 9beta 1 and alpha 4beta 1 providing a novel mechanism for regulating cell adhesion by alternative splicing. 1183 64

Ethyl acetate, 2-methylbutyl acetate, butyl acetate (BA), and hexyl acetate were detected by solid-phase microextraction and gas-liquid chromatography inside slices of Golden Delicious apple and in water droplets on the skin of slices incubated in sealed glass jars. Conidial adhesion and germination of the gray mold fungus, Botrytis cinerea, was assessed on apple slices after exposure or no exposure to the esters in the headspaces of glass jars. Attached conidia were dislodged by sonication and remaining conidia on apple slices were counted by microscopy. Adhesion generally increased as BA increased to 7.2 microg mL(-1), but declined with greater concentrations. BA at 0-3.6 microg mL(-1) for 24 h stimulated adhesion 2-fold greater compared to that at 4 h. Adhesion stimulated by BA increased as a function of time (0-24 h), showing linear trends (r (2) = 0.99; p = 0.01) during 0-12 h. The four esters were similar in their ability to stimulate adhesion. Germination of conidia exposed to BA increased linearly (r (2) = 0.95-0.98; p = 0.01) during 4-12 h. Conidial adhesion stimulated by BA preceded conidial germination by 2 h. The four esters stimulated conidial germination to similar levels. Results indicated that acetate esters formed in apple fruit are mycoactive, influencing life-cycle events of B. cinerea important to its survival on the fruit. The similar responses of three B. cinerea isolates to four acetate esters suggests a common stimulation mechanism may operate in B. cinerea.
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PMID:Mycoactive acetate esters from apple fruit stimulate adhesion and germination of conidia of the gray mold fungus. 1200 75

Molloplast-B is a widely used, long-term, heat-cured silicone denture soft lining material. Adhesion failure of this material to poly (methyl methacrylate) denture base material is a problem that is encountered clinically. The purpose of this study was to investigate the effects of long-term immersion in water at 37 +/- 1 degrees C and of accelerated ageing in water at 50 +/- 1 degrees C on the tensile and shear bond strength values of this denture resilient lining material bonded to a heat-cured denture base material. Immersion in water for 1 week at 37 +/- 1 degrees C had no significant effect on the measured bond strength values. Longer immersion of specimens in water at 37 +/- 1 degrees C led to a significant reduction in the measured tensile and shear bond strengths. The present study has demonstrated that the reduction in Molloplast-B bond strength that occurs as a result of long-term ageing in water at 37 +/- 1 degrees C can be achieved in a shorter period of time by ageing the specimens in water at a higher temperature.
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PMID:Effect of ageing on the bond strength of a permanent denture soft lining material. 1242 31

Mononuclear cell infiltration of the thyroid is a prominent feature of chronic lymphocytic thyroiditis. Adhesion molecules play a major role in determining the localization of inflammatory mononuclear cells in the thyroid. Previous reports from animal models and human studies have described the thyroidal expression of adhesion molecules only late in clinical disease. In this study, we examined the distribution and kinetics of expression of E-selectin, VCAM-1, LFA-1, and ICAM-1 in the NOD-H2h4 mouse, a model of spontaneous autoimmune thyroiditis accelerated by dietary iodine. Mice were fed 0.015% NaI in their drinking water for 2, 4, 6, 8, and 16 weeks, and thyroids were removed, serially sectioned, and stained in an avidin-biotin-peroxidase assay. We found a dramatic increase in E-selectin and VCAM-1 expression on intrathyroidal endothelial cells after 16 weeks of iodine treatment. In addition, we describe for the first time that thyrocytes from the NOD-H2h4 mouse, and the parental NOD, constitutively express ICAM-1 independent of iodine treatment and prior to mononuclear cell infiltration of the thyroid gland. ICAM-1 was not detected on the thyrocytes of other untreated strains of mice, implicating expression of this adhesion molecule as a critical event in the recruitment of inflammatory mononuclear cells to the thyroid.
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PMID:Adhesion molecules as susceptibility factors in spontaneous autoimmune thyroiditis in the NOD-H2h4 mouse. 1256 90

The aim of this study was to examine the influence of polymyxin B on the level of expression of adhesion molecules E-selectin, ICAM-1, and VCAM-1 on human vascular endothelium activated with B. fragilis endotoxins or enterotoxin. Lipopolysaccharides were extracted by phenol-water method from one nonenterotoxigenic (NTBF) and three enterotoxigenic (ETBF) B. fragilis strains. LPS preparations were purified with nucleolytic enzymes and ultracentrifugation. Enteotoxin (BFT) was prepared from the supernatant of reference B. fragilis ATCC 43858 culture by precipitation with ammonium sulphate. BFT preparations were purified with the application of ion-exchange chromatography and hydrophobic chromatography. Adhesion molecule expression on the surface of human vascular endothelial cells (HMEC-1 cell line) was determined after simultaneous stimulation with bacterial compounds at the concentration of 10 micrograms/ml and polymyxin B at the concentration of 20 micrograms/ml. Endothelial cells were activated for 4 hours (E-selectin expression) or for 24 hours (ICAM-1 and VCAM-1 expression). Adhesion molecules were detected in immunoenzymatic test (ELISA) with the use of mouse, monoclonal antibodies against human ICAM-1, VCAM-1, and E-selectin. The results of performed experiments suggest, that polymyxin B changes the level of adhesion molecule expression on human vascular endothelium. This antibiotic causes changes in the expression of endothelial ICAM-1, VCAM-1, and E-selectin during simultaneous stimulation of endothelium with B. fragilis endotoxins or enterotoxin. In the majority of cases the addition of polymyxin B leads to the up-regulation of examined adhesion molecules.
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PMID:[Stimulation of adhesion molecule expression in human vascular endothelium by Bacteroides fragilis toxins--influence of polymyxin B]. 1263 59

Adhesion problems during tablet manufacturing have been observed to be dependent on many formulation and process factors including the run time on the tablet press. Consequently, problems due to sticking may only become apparent towards the end of the development process when a prolonged run on the tablet press is attempted for the first time. It would be beneficial to predict in a relative sense if a formulation or new chemical entity has the potential for adhesion problems early in the development process. It was hypothesized that favorable intermolecular interaction between the drug molecules and the punch face is the first step or criterion in the adhesion process. Therefore, the rank order of adhesion during tablet compression should follow the rank order of these energies of interaction. The adhesion phenomenon was investigated using molecular simulations and contact mode atomic force microscopy (AFM). Three model compounds were chosen from a family of "profen" compounds. Silicon nitride AFM tips were modified by coating a 20-nm iron layer on the surfaces by sputter coating. Profen flat surfaces were made by melting and recrystallization. The modified AFM probe and each profen surface were immersed in the corresponding profen saturated water during force measurements using AFM. The work of adhesion between iron and ibuprofen, ketoprofen, and flurbiprofen in vacuum were determined to be -184.1, -2469.3, -17.3 mJ. m(-2), respectively. The rank order of the work of adhesion between iron and profen compounds decreased in the order: ketoprofen > ibuprofen > flurbiprofen. The rank order of interaction between the drug molecules and the iron superlattice as predicted by molecular simulation using Cerius(2) is in agreement with the AFM measurements. It has been demonstrated that Atomic Force Microscopy is a powerful tool in studying the adhesion phenomena between organic drug compounds and metal surface. The study has provided insight into the adhesion problems occurring during tablet compression and a direction for continued study.
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PMID:Modeling of adhesion in tablet compression--I. Atomic force microscopy and molecular simulation. 1266 Oct 65

Adhesion of Pseudomonas sp. NCIMB 2021 was tested on different non-solid hydrogel surfaces under different shear conditions. Gels consisting of alginate (highly anionic), chitosan (highly cationic), modified polyvinyl alcohol PVA-SbQ (very low cationic) and agarose (neutral) were casted in moulds custom-made for a rotating annular biofilm reactor. Cells were stained with SYBRR Green I nucleic acid gel stain, and images were collected using a confocal laser scanning microscope. Relative adhesion was quantified by determining percent cell coverage using image analysis. Bacterial adhesion on gels decreased at higher shear rates. At low shear rates, adhesion varied significantly between different gels, in the following descending order: alginate > agarose > chitosan > PVA-SbQ. Only adhesion to alginate remained significantly higher than to the others at high shear rates. Lowest cell coverage at all shear rates was recorded on PVA-SbQ gels. Clearly, the macroscopic hydrophobicity of the hydrogel surfaces did not enhance adhesion as observed for solid surfaces. A 5% PVA-SbQ gel showed the most promising antifouling properties.
Water Res 2003 Feb
PMID:Adhesion of the marine bacterium Pseudomonas sp. NCIMB 2021 to different hydrogel surfaces. 1268 85

Poly(ethylene oxide) brushes have been covalently bound to glass surfaces and their presence was demonstrated by an increase in water contact angles from fully wettable on glass to advancing contact angles of 54 degrees, with a hysteresis of 32 degrees. In addition, electrophoretic mobilities of glass and brush-coated glass were determined using streaming potential measurements. The dependence of the electrophoretic mobilities on the ionic strength was analyzed in terms of a softlayer model, yielding an electrophoretic softness and fixed charge density of the layer. Brush-coated glass could be distinguished from glass by a 2-3-fold decrease in fixed charge density, while both surfaces were about equally soft. Adhesion of Staphylococcus epidermidis HBH276 to glass in a parallel plate flow chamber was extremely high and after 4 h, 19.0 x 10(6) bacteria were adhering per cm2. In contrast, the organisms did not adhere to brush-coated glass, with numbers below the detection limit, i.e. 0.1 x 10(6) per cm2. These results attest to the great potential of polymer brushes in preventing bacterial adhesion to surfaces.
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PMID:Characterization of poly(ethylene oxide) brushes on glass surfaces and adhesion of Staphylococcus epidermidis. 1274 72


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