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Query: UMLS:C0001511 (Adhesion)
 5,955 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of this study was to examine the hydrophobicities of 23 urogenital, dairy, poultry, and American Type Culture Collection isolates of lactobacilli and to determine the effect on hydrophobicity of serially passaging the strains in liquid medium. To this end, strains were grown after isolation and identification and then serially passaged up to 20 times. Hydrophobicity was assessed through contact angle measurements on lawns of cells by using water, formamide, methylene iodide, 1-bromonaphthalene, and hexadecane as wetting agents and through measurement of their partitioning in a hexadecane-water system. The hydrophobicities of these strains varied widely, with Lactobacillus casei strains being predominantly hydrophilic and L. acidophilus strains being mostly hydrophobic. For some isolates, serial passaging was accompanied by a clear loss of hydrophobic surface properties, whereas for other strains, cultures became heterogeneous in that some cells had already lost their hydrophobic surface properties while others were still hydrophobic. Adhesion of this collection of lactobacilli to hexadecane droplets in microbial adhesion to hexadecane (MATH) tests was driven by their aversion to water rather than by their affinity for hexadecane, as concluded from the fact that hexadecane contact angles were zero for all strains. Furthermore, adhesion of the lactobacilli to hexadecane in MATH tests occurred only when the water contact angle on the cells was above 60 degrees.
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PMID:Comparison of contact angles and adhesion to hexadecane of urogenital, dairy, and poultry lactobacilli: effect of serial culture passages. 162 24

In recent years, polysaccharide-based films have been developed for many applications. Some of these are in the pharmaceutical industry, where the adhesion of microorganisms to surfaces is a concern. After adhesion of a microorganism to a solid surface has taken place, the subsequent biofilm formed can act as a vehicle for spreading infections. The aim of this study is to compare the bacterial adhesion of E. coli and S. aureus from a contaminated solid model (Tryptone Soya Agar) to a range of polysaccharide-based films. These polysaccharide-based films consist of different natural starches (potato, cassava, wheat, pea and rice) and synthetic polymers hydroxyl-propyl cellulose (HPC) and carboxyl methyl cellulose (CMC)). The surface energy parameters of the films were calculated from the contact angle measurements by the sessile drop method. Apolar and polar liquids (water, formamide and hexadecane) and the Lifshitz-Van der Waals/acid-base (LW/AB) approach were used according to the method of Van Oss, Chaundhury and Good. The surface properties of the films were also correlated to the microbial adhesion. This indicated that, for both E. coli and S. aureus, the surface roughness did not affect the microbial adhesion. Only gamma(sAB) had any correlation with the microbial adhesion and gamma(sLW) was almost constant for all the various polysaccharide films tested. In addition, the electron-donor properties of the materials, exhibited via gamma(s+), were positively correlated with the adhesion of S. aureus but not with E. coli. This was in agreement with the results of the MATS (Microbial Adhesion To Solvents) test performed on the two bacteria. This revealed that only S. aureus presented an electron-acceptor characteristic.
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PMID:An investigation of microbial adhesion to natural and synthetic polysaccharide-based films and its relationship with the surface energy components. 1871 4