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Query: UMLS:C0001511 (Adhesion)
 5,955 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this study we present evidence that the mouse and rat sialoadhesin (originally named sheep erythrocyte receptor) on macrophages can function as a lymphocyte adhesion molecule. Lymphocytes were shown to bind to the splenic marginal zone, and lymph node subcapsular sinus and medulla in a frozen section assay. Selective depletion experiments showed that binding was mediated by macrophages. Adhesion was blocked by preincubation of the sections with monoclonal antibodies against mouse or rat sialoadhesin. Binding was temperature dependent, divalent cation independent, and involved sialic acid residues on the lymphocyte, as it could be inhibited by prior neuraminidase treatment or addition of the ganglioside GD1a. Binding to sialoadhesin was confirmed using the purified receptor and was observed among T cells, T blasts, B cells, and B blasts. Isolated macrophages or dendritic cells showed little binding. Sialoadhesin provides the first example of a macrophage-restricted lymphocyte adhesion molecule.
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PMID:Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule. 151 34

Previous investigations established that focal subretinal injections of neuraminidase, chondroitinase, and hyaluronidase in the rabbit lead to a diffuse loss of retinal adhesiveness beyond the site of injection. This loss of adhesiveness, measured by peeling of the retina immediately after enucleation, correlates with changes in the interphotoreceptor matrix (IPM), as monitored by lectin histochemistry. In this study, rabbits were evaluated during recovery of retinal adhesiveness after subretinal injections of neuraminidase and chondroitinase. Adhesion recovered steadily 5-20 days after chondroitinase injection. After administration of neuraminidase, adhesion remained low for approximately 14 days but recovered to normal by 20 days. The recovery of adhesiveness correlated closely with reestablishment of the normal distribution of peanut agglutinin-binding glycoconjugates in the IPM, one group of molecules thought to participate in retinal adhesion. Electroretinography and light microscopy showed no abnormalities in the retina or retinal pigment epithelium after recovery. These results suggest that IPM glycoconjugates participate in maintaining retinal adhesion.
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PMID:Recovery of retinal adhesion after enzymatic perturbation of the interphotoreceptor matrix. 154 77

Castanospermine (CSP), an inhibitor of alpha-glucosidase, enhanced immunoglobulin (Ig) release in a Staphylococcus aureus Cowan I (SAC)-induced lymphocyte culture (Scand. J. Immunol. 1990. 32: 529). In a pokeweed mitogen (PWM)-human lymphocyte culture, unlike the SAC-stimulated system, CSP strongly decreased the number of IgG-, IgA- and IgM-secreting cells as well as that of Ig-bearing cells. Peripheral blood lymphocytes treated with swainsonine, a mannosidase II inhibitor, or with neuraminidase also showed a reduced response to PWM. In cross-culture experiments, only a mixture of B cells pretreated with either agent and untreated T cells showed such a suppressive effect. Adhesion was decreased between B cells treated with either agent and untreated T cells, but not between treated T cells and untreated B cells. These results demonstrate that a certain alteration in B cell membrane oligosaccharides inhibited the T cell-B cell adhesion in the PWM culture, leading to an arrest of B cell maturation. Considering that these inhibitors eventually prevent terminal sialic acid addition, the present study provides evidence that sialic acids on B cell surface oligosaccharides play a biological role in the T cell-B cell interaction.
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PMID:Glycosidase inhibitors (castanospermine and swainsonine) and neuraminidase inhibit pokeweed mitogen-induced B cell maturation. 163 2

This study surveyed some adhesive properties of strains of Fusobacterium nucleatum representative of the 3 recently defined groups or subspecies that could relate to their colonization and virulence. With one exception, F. nucleatum strains agglutinated sheep erythrocytes, but the quantity of bacteria required and the sensitivity of the hemagglutination reactions to inhibition by 0.05 M galactose or arginine varied between strains, and did not exhibit clear-cut correlations with subspecies. Neuraminidase treatment of erythrocytes generally enhanced the hemagglutinating activity of most strains, but trypsin treatment had no effect. Strains of F. nucleatum also attached in moderate numbers to buccal epithelial cells. Treatment of the epithelial cells with neuraminidase or with trypsin increased the numbers of all Fusobacterium strains that attached. Treatment of hydroxyapatite (HA) beads with submandibular or parotid saliva also promoted the adhesion of all strains of F. nucleatum studied. Treatment of HA with human serum or albumin produced a selective effect. Adhesion of some strains was promoted by serum and albumin treatment, and that of other strains was unaffected. Adhesion of all strains of F. nucleatum was enhanced to statherin-treated HA, whereas HA treated with salivary proline-rich protein-1 did not foster F. nucleatum attachment. Three of 4 strains of the subspecies vincentii, and each of 2 polymorphum strains studied exhibited strong adhesion to HA treated with either human type I or type IV collagen. However, only 1 of 5 strains of the subspecies nucleatum bound well to collagen-treated HA.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Adhesive properties of strains of Fusobacterium nucleatum of the subspecies nucleatum, vincentii and polymorphum. 182 May 61

Adhesion of P. aeruginosa to normal and injured rat tracheas was examined. Rat tracheas were injured by exposure to 0.1N HCl for 10 min, and incubated with P. aeruginosa. Adhesion was quantitated by direct count of the number of bacteria attached to a fixed surface area as viewed by scanning electron microscopy. P. aeruginosa adhered to injured tracheas much more than to normal tracheas. The adhesion of P. aeruginosa, preincubated with mucin and sugars, to acid injured trachea was examined. Mucin, N-acetylneuraminic acid and N-acetyl-D-galactosamine inhibited the adhesion of P. aeruginosa to injured tracheas, but not N-acetylglucosamine, L-fucose, D-mannose and D-galactose. Periodate oxidation and neuraminidase treatment of acid injured tracheas reduced the adhesion of P. aeruginosa. These data suggest that N-acetylneuraminic acid (sialic acid) is the receptor for P. aeruginosa or a part of the receptor in acid injured rat trachea and in tracheobronchial mucin.
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PMID:[Study of the receptor for P. aeruginosa on tracheal cells and in tracheobronchial mucin]. 250 16

A reproducible and standardized assay for measuring the cytoadherence of knobby Plasmodium falciparum-infected red cells to amelanotic melanoma cells was developed. Adhesion was dependent on temperature, haematocrit, and parasitaemia. Addition of EDTA to the binding medium reduced adhesion. Removal of protease-sensitive molecules on the surface of the infected cell abolished cytoadherence, whereas removal of carbohydrate residues by treatment of cells with neuraminidase or galactosidase promoted adhesion. Calcium, magnesium, fibrinogen or fibronectin in the medium had no effect on adhesion nor was there any enhancement of adhesion by pre-loading infected cells with calcium. Serum was essential for good adhesion. Adhesion was species specific for target cells; human endothelial or amelanotic melanoma cells were suitable target cells whereas bovine cells were not. The amelanotic melanoma cell could be formalin-fixed and still retain its adhesion properties. The binding properties of formalin-fixed amelanotic melanoma cells were not identical to those of endothelial or unfixed target cells.
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PMID:In vitro cytoadherence of Plasmodium falciparum-infected erythrocytes to melanoma cells: factors affecting adhesion. 267 75

The effect of Tamm Horsfall protein (THP) of 18 healthy subjects and 14 diabetics on adherence of Escherichia coli (06:K13) 2699 strain to human kidney cells (HUK) was studied. Adhesion of bacteria (without additions: 100 bacteria per cell) was reduced dose-dependently by THP, half maximal inhibition occurring with 250 micrograms THP ml-1. Maximal inhibition (-84% at 1000 micrograms ml-1) exceeded inhibition by alpha-methyl-mannoside (36% at 50 mM), was specific (not reproduced by other glycoproteins, e.g. ovalbumin, mucin or thyroglobulin) and reversible (abolished by washing THP off HUK cells). Anti-adherence property of THP was not abolished by neuraminidase treatment. No significant difference of anti-adherence activity of THP was found between controls and diabetics, despite altered carbohydrate composition of THP in diabetes.
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PMID:Tamm Horsfall glycoprotein interferes with bacterial adherence to human kidney cells. 313 Feb 65

Single viable muscle fibers isolated from adult rats by collagenase digestion rapidly bind dissociated spinal neurons or PC-12 cells but not a variety of other cells tested. The adhesion process is calcium-independent, temperature-sensitive, and is not blocked by pretreating cells with inhibitors of energy metabolism or actin polymerization. Adhesion is mediated by a carbohydrate-binding protein and can be inhibited by N-acetylneuraminic acid or mucin, a glycoprotein with high sialic acids content. The hapten inhibitors do not dissociate cells if added after aggregation has occurred. Experiments to block adhesion by pretreatment of cells with either neuraminidase or mucin show that the sialic acids-rich moiety is on the nerve cells, while its receptor is on the muscle fibers.
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PMID:Rapid adhesion of nerve cells to muscle fibers from adult rats is mediated by a sialic acid-binding receptor. 371 Nov 46

Fixed human erythrocytes were used as model particles for the study of adhesion and phagocytosis by rat peritoneal macrophages. Erythrocytes were fixed with various concentrations of glutaraldehyde or tannic acid, or were treated with neuraminidase. Adhesion and phagocytosis of these cells were measured. In addition, the surface energy of these erythrocytes and macrophages was estimated by the contact angle technique. Free energies of adhesion, based on the cell surface energies, were correlated with both adhesion and phagocytosis.
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PMID:Adhesion, phagocytosis and cell surface energy. The binding of fixed human erythrocytes to rat macrophages and polymethylpentene. 717 84

Adhesion of glutaraldehyde-fixed human red blood cells (RBC) to polystyrene was measured as a function of concentration of mono-, di-, and trivalent cations. The effectiveness for preventing adhesion was trivalent > divalent > monovalent. Adhesion increased with ionic strength but no specificity of cations in a particular valency group was observed. Adhesion as a function of NaCl concentration was not affected by temperature between 1 degrees C and 22 degrees C but decreased in 150 mM NaCl above pH 6. Chicken RBC and neuraminidase-treated human RBC were more adhesive than untreated human RBC in NaCl. In 150 mM NaCl, adhesion of human RBC decreased in the presence of hemolyzate or polyethylene glycols (mol. wt. 10,000-300,000). These results support the hypothesis that RBC adhesion to polystyrene occurs in the primary minimum of the potential energy of interaction and is governed by attractive London-Van der Waals and repulsive electrostatic forces. The implications were applied to study of hemagglutination of RBC with lectin and lectin-labeled gold granules in polystyrene microplates.
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PMID:Adhesion of human and chicken red blood cells to polystyrene: influence of electrolyte and polyethylene glycol concentration. 719 3


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