Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0001511 (Adhesion)
 5,955 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The skeletal muscle capillary bed may be an ideal recipient site for transplantation of genetically modified autologous endothelial cells and thus provide a basis for a technique of somatic gene therapy that would be applicable to a variety of acquired and inherited human diseases. The purpose of this study was to test the hypothesis that adhesion of lac-Z-transduced microvascular endothelial cells (MVEC) in the skeletal muscle capillary bed in vivo is dependent on the duration of arterial occlusion after injection of the transduced MVEC. MVEC derived from the abdominal fat pad of syngeneic rats (Wistar F-455) were transfected with the BAG vector, a replication-incompetent retroviral vector containing the lac-Z gene for beta-galactosidase and the Tn5 gene for selection of the transduced cells by the neomycin analogue, G418. lac-Z-transduced MVEC were radiolabeled with 125I-PKH-95, and, after the femoral artery was occluded for 10 min, these cells (1 to 2 x 10(6)) were injected intraarterially into the rat hindlimb. In the experimental groups the femoral artery clamp was removed at 0, 60, or 120 min after injection. A control group without pre- or postinjection femoral arterial occlusion was also studied. Adhesion of MVEC in the skeletal muscle capillary bed (mean percentage of injected 125I activity) was determined in groups of 4 rats at 1 day, 1 week, and 1 month after injection. Adhesion of the transduced MVEC did not increase as the duration of femoral artery occlusion after injection was increased.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Transplantation of lac-Z-transduced microvascular endothelial cells into the skeletal muscle capillary bed of the rat hindlimb occurs independent of the duration of femoral artery occlusion after injection of cells. 799 42

In this study, optical techniques were used to characterize adhesion of hematopoietic cells to bone marrow (BM) stromal microenvironment in situ. Bone marrow cells (BMC) labeled with PKH membrane linkers were infused into nonconditioned femurs and were monitored by fluorescence microscopy through an optical bone window. Repeated infusions of BMC into the femoral lumen resulted in a progressive increase in the number of adherent cells (p < 0.01), indicating that the availability of hemopoietic niches in the nonconditioned BM was not a rate-limiting factor of early BMC seeding. Adhesion of hemopoietic progenitor and stem cells (HSPC) was 30-fold higher than lineage(+) BMC (p < 0.001), suggesting that adhesion molecules on the surface of HSPC have a higher propensity for adhesion. BMC antigen-matched to and disparate from BM stroma adhered at equal rates, opposing the idea of involvement of antigen barriers during early seeding. It is concluded that primary adhesion to BM stromal microenvironment is favorable for HSPC and is not restricted by antigenic barriers or availability of vacant niches.
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PMID:Antigen barriers or available space do not restrict in situ adhesion of hemopoietic cells to bone marrow stroma. 1179 25