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Query: UMLS:C0001511 (Adhesion)
 5,955 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The tetrapeptide, Arg-Gly-Asp-Ser (RGDS), which corresponds to a core sequence of cell adhesion proteins, was coimmobilized with insulin on to surface-hydrolyzed poly(methyl methacrylate) film. Adhesion of STO mouse fibroblast cells was enhanced by the immobilization of RGDS, but not of insulin. On the other hand, growth of the cells was accelerated by the insulin immobilization, but not by the RGDS immobilization. Coimmobilization of insulin and RGDS did not affect cell adhesion but accelerated cell growth remarkably. This acceleration effect is considered to be attributable to a prolonged interaction of immobilized insulin and insulin receptor by adhesion enhancement, and to a postulated interaction between activated insulin receptor and integrin.
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PMID:Cell growth on insulin/RGDS-coimmobilized poly(methyl methacrylate) films. 860 88

Because IL-3-dependent multipotential FDCP-Mix cells expressing human colony-stimulating factor-1 (CSF-1) receptor did not proliferate in response to soluble CSF-1, we investigated whether their proliferation would be induced in co-culture with adherent cells expressing the membrane form of CSF-1 (MemCSF-1). FDCP-Mix cells with high CSF-1R expression (NAF21 cells) were placed on stromal MS-5 cells or STO fibroblasts expressing MemCSF-1 (2M-1 cells and STO-M2 cells, respectively), in absence of IL-3. NAF21 cells bound significantly to 2M-1 cells as compared to control FDCP-Mix cells. Adhesion of NAF21 cells was inhibited by anti-huCSF-1 antibodies, as well as anti-huCSF-1R antibodies. Interestingly, NAF21 cells proliferated on both 2M-1 and STO-M2 cells but with very different kinetics. Moreover, NAF21 cell proliferation was also supported by glutaraldehyde-fixed 2M-1 cells or highly concentrated MS-5 cell culture supernatant, but not by CSF-1 coated on culture dishes. These results strongly suggest that MemCSF-1/CSF-1R interaction mediates a specific adhesion of NAF21 cells to stromal cells and allows stimulation of hematopoietic cells by stromal cell-derived factors expressed in a membrane-bound form or concentrated within the extracellular matrix. Thus, cytokine receptors deficient in mitogenic signalling may nevertheless have a regulatory role in hematopoietic progenitor cell proliferation by acting as adhesion molecules.
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PMID:Role of the membrane form of human colony-stimulating factor-1 (CSF-1) in proliferation of multipotent hematopoietic FDCP-mix cells expressing human CSF-1 receptor. 1094 43

Insulin or albumin was immobilized on collagen beads using water-soluble carbodiimide. Adhesion of STO mouse fibroblast cells onto the beads decreased with increasing the amount of immobilized proteins. Growth of the cells was remarkably accelerated on the insulinimmobilized collagen beads, which can be used for serum-free cell culture. The growth acceleration became larger with increasing the amount of immobilized insulin, while it became smaller with increasing the amount of immobilized albumin. In addition, the immobilized insulin more strongly accelerated the cell growth than free insulin plus collagen beads. (c) 1995 John Wiley & Sons, Inc.
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PMID:Serum-free cell culture on insulin-immobilized porous collagen beads. 1862 96