Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0001430 (adenoma)
 21,222 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Proteoglycans (PGs) were localised immunohistochemically in 52 salivary gland tumours including pleomorphic adenoma, adenoid cystic carcinoma, acinic cell carcinoma, oncocytoma, mucoepidermoid carcinoma, clear cell tumour and Warthin tumour, using antibodies raised against large PG, small PG, chondroitin 4-sulphate PG, chondroitin 6-sulphate PG, heparan sulphate PG and keratan sulphate PG. Large PGs were mainly observed in mucinous materials of extracellular matrix (ECM) and interstitial fibrous element of tumour tissues, while small PGs were located only in hyaline matrix and surrounding fibrous (capsular) connective tissues. Chondroitin 6-sulphate PG was detected in the ECM of pleomorphic adenomas and clear cell carcinomas and in pseudocystic spaces of adenoid cystic carcinomas, but only in vessel walls in non-neoplastic tissues. Keratan sulphate PG was observed to locate in mucinous material of pleomorphic adenomas, acinic cell carcinomas and clear cell carcinomas, but not in the adenoid cystic carcinomas examined, and it was also unobservable in non-neoplastic salivary gland tissues. Heparan sulphate PG was observed on the inner surfaces of true ductal spaces of adenoid cystic carcinomas and on cell surfaces of oncocytoma cells. By HPLC analysis, individual glycosaminoglycans contained in tumour tissues were compared. Chondroitin 6-sulphate PG was very rich in ECM of pleomorphic adenomas and adenoid cystic carcinomas. Pleomorphic adenomas contained relatively more low-sulphated chondroitin sulphate than adenoid cystic carcinomas and other tumours.
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PMID:Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours. 190 86

One of the common histopathologic features of pleomorphic adenoma (PA) and adenoid cystic carcinoma (ACC) of the salivary gland is the abundant accumulation of glycosaminoglycans (GAGs). To study a relationship between the histologic distribution of GAGs and the morphologic pattern of salivary gland tumors, we carried out immunohistochemical and biochemical analyses of GAGs on 16 cases of PA and three cases of ACC. Immunohistochemically, several GAG fractions such as chondroitin-6-sulfate, unsulfated chondroitin sulfate, and keratan sulfate were seen mainly at the plasma membrane of both PA and ACC cells and of myoepithelial cells of normal salivary glands. Most of these GAG-positive cells were distributed in solid and chondromyxoid parts of PA and in the cellular part of ACC. Biochemical GAG analysis of both PA and ACC, separated into nonsulfated and sulfated fractions by two-dimensional electrophoresis, revealed high concentrations of chondroitin sulfate in both neoplasms, but the concentration of hyaluronic acid, was significantly higher in PA than in ACC. The results show that the concentration of hyaluronic acid, a major nonsulfated GAG fraction, was significantly higher in PA than in ACC and, therefore, this difference may be useful in the differentiation of the two neoplasms.
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PMID:Glycosaminoglycans in pleomorphic adenoma and adenoid cystic carcinoma of the salivary gland. 217 71

The tumor matrix of salivary pleomorphic adenoma (PA) is characteristically rich in glycosaminoglycans (GAGs), which contribute to its complex histoarchitecture. This study evaluated the microscopic localization of various GAGs in 17 PAs, using a panel of anti-GAG monoclonal antibodies and biotinylated hyaluronic acid (HA)-binding protein. Both epithelial and mesenchymal-like tissues were confirmed to contain GAGs. Luminal epithelial cells mostly lacked GAGs, whereas GAGs were seen both in the cytoplasm and cell membrane of non-luminal epithelial cells. In addition, small intercellular accumulations of GAGs were often present in solid epithelial areas, implying the epithelial origin of GAGs. GAGs did not appear to be a main component of the hyaline matrix. The myxoid region was consistently stained for both chondroitin 6-sulfate (CS-6) and HA but variably for chondroitin 4-sulfate (CS-4), dermatan sulfate (DS) and keratan sulfate (KS); heparan sulfate (HS) was not detected. The chondroid region showed increased staining for CS-6 but reduced staining for HA when compared with the myxoid region. In addition, CS-4, DS and KS were seen both in chondroid cells and the territorial matrix, whereas HS was present only in the cells. It is suggested that GAGs in PA are mainly produced by non-luminal cells and influence the proliferation, differentiation, secretory activity and shape of tumor cells, thus contributing to the morphological diversity of this tumor.
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PMID:Localization of glycosaminoglycans (GAGs) in pleomorphic adenoma (PA) of salivary glands: an immunohistochemical and histochemical evaluation. 970 80