Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0001430 (adenoma)
 21,222 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The periodic acid-thionin Schiff/potassium hydroxide/periodic acid-Schiff (PAT/KOH/PAS) procedure has been used to investigate the histochemical staining characteristics of the mucins found in adenocarcinoma and villous lesions of the large intestine. The 46 blocks examined represented 58 lesions from 37 patients, all of whom had had resections for carcinoma of the colon. tin sharp contrast to normal colon, none of the adenocarcinomas stained red with the PAT/KOH/PAS. With two exceptions the poorly and moderately differentiated adenocarcinomas stained blue, whereas of the well differentiated lesions half were blue and half purple. The malignant villous lesions demonstrated the same trends, although a larger percentage were purple. None of the benign lesions stained blue. It is suggested that malignancy in the colon is accompanied by an increase in blue staining in the PAT/KOH/PAS technique and that such staining may be of value in the interpretation of highly atypical adenoma where it might identify the onset of malignancy. This change in staining indicates a distinct alteration in the chemistry of the mucins which we interpret as a reduction in the degree of side chain O-acylation of their constituent sialic acids.
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PMID:A new histochemical technique of use in the interpretation and diagnosis of adenocarcinoma and villous lesions in the large intestine. 7 51

Immunohistochemical staining and histochemical approach for mucin were applied in studying 58 cases of colorectal adenomas. Among them, there were 13 cases of multiple adenomas (polyps), 5 cases of inflammatory polyps and 5 cases of juvenile polyps. The results indicated that positive expression of both McAb MC5 and Ulex europaeus agglutinin-1(UEA-1) were correlating with the degree of dysplasia, the histological type and the size of adenomas. The positive expression of peanut agglutinin (PNA) was correlating with the degree of dysplasia. Moderate and heavy stainings were mainly seen in adenomas accompanying with moderate and severe dysplasia, as well as adenomas with early carcinomatous changes. PAT-KOH-PAS method could sensitively reflect the occurrence of dysplasia and malignancy of the adenomas. These findings support the concept of an adenoma(dysplasia)-carcinoma sequence. In comparing multiple adenomas (number of adenomas > 100) with the solitary ones, a notable difference was obtained in the expression of McAb MC5. It's concluded that combined use of certain immunohistochemical and histochemical stainings is well be useful in detecting the malignant potentiality of adenoma.
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PMID:[Immunohistochemical and histochemical study on colorectal adenomas and polyps]. 133 45

Using HE, AB/PAS, HID/AB, PAT/KOH/PAS stain and CEA monoclonal antibody BA stain techniques, composition of mucin and distribution of CEA in the tumor tissue were detected in 65 cases of colorectal tubular adenoma, 7 adenoma with malignant change and 14 established tubular adenocarcinoma in order to study the functional changes of colorectal adenoma and its relation with the colorectal adenocarcinoma. According to Konishi's criteria, colorectal tubular adenomas were divided into three groups: mild, moderate and severe dysplasia, added with two of ours: adenoma with malignant change and established adenocarcinoma, totalling 5 groups for comparison. The results showed that CEA positive rate increased with the grade of dysplasia in these 5 groups (33%, 57%, 67%, 86% and 100%). Similarly, the percentage of non-acetylated acid and C7 C9 O-acetylated sialomucin also gradually increased with the grade of dysplasia, showing a parallel tendency. Epithelial cells, secreting a heterogeneous mucin, could be taken as a signal of abnormal cellular differentiation. The increase of non-acetylated acid and C7 C9 O-acetylated sialomucin in colorectal adenoma implies a likelihood of malignant change.
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PMID:[Functional change of colorectal adenoma and its relation with colorectal adenocarcinoma--application of mucin histochemical and immunohistochemical techniques]. 345 39

To assess the mucin changes associated with the development of colorectal cancer, the periodic acid-thionin schiff (PAT)/KOH/PAS staining properties of 38 advanced carcinomas, 8 submucosal invasive carcinoma, 29 mucosal carcinomas (adenomas with severe atypia), 19 adenomas with moderate atypia and 38 adenomas with mild atypia were studied with special attention to the heterogeneity of staining property within each lesion. 6 submucosal carcinomas and 28 mucosal carcinomas were judged to have benign adenomatous portion using hematoxilin-eosin staining. The more advanced the carcinomas, or the higher the grade of atypia of adenomas, the fewer the lesions of which goblet cell mucus (GCM) and the mucus at the luminal surface or that in the luminal accumulation (SC) stained red. SC frequently stained bluer than GCM in highly atypical glands. The staining properties of benign adenomatous portions concomitant with submucosal or mucosal carcinomas were similar to those of benign adenomas, but not to their carcinomatous foci. These findings suggest that the changes in the PAT/KOH/PAS staining property associated with the development of colorectal carcinoma mainly occur in SC, and that the majority of submucosal and mucosal carcinomas were 'carcinoma in adenoma'.
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PMID:[The heterogeneity of periodic acid-thionin schiff/KOH/PAS staining property and the histological grading of atypia in colorectal carcinoma and adenoma]. 750 21

Changes of surface sugar residues in the large intestinal mucosa may be associated with malignant transformation and may be of importance in differentiating borderline lesions. To compare these changes in normal mucosa, adenomas, and carcinomas of the large intestine, we investigated modifications of carbohydrate composition, such as those of N-acetyl-galactosamine (GalNac), L-fucose, and o-acetylated sialic acid, by histochemical staining with Dolichos biflorus agglutinin (DBA) and Ulex europaeus agglutinin-1 (UEA-1) lectins, and with Culling's periodic acid-thionin Schiff/potassium hydroxide/periodic acid-Schiff (PAT/KOH/PAS), respectively. For stable staining, the sections stained with DBA and UEA-1 were pretreated with potassium hydroxide and neuraminidase. We conclude that the pattern of the two lectin stainings in carcinomas is quite different from that in normal mucosa and adenomas, and that it shows the carcinomatous features in some cases of adenoma with severe atypia (borderline lesions). In contrast, PAT/KOH/PAS staining demonstrates differences between normal mucosa and adenomas rather than differences between adenomas and carcinomas.
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PMID:Altered carbohydrate composition in colorectal adenomas and carcinomas: histochemical characterization of N-acetylgalactosamine, L-fucose, and o-acetylated sialic acid. 880 25