Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: KEGG:D06457 (HCG)
 2,659 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mesterolone, HCG and PMS, clomiphene and various other drugs were administered to 236 subfertile patients with disturbed spermatogenesis. Most of the patients received mesterolone. The effects of the various therapeutic regimes are discussed. Improvement of the sperm count and spermatozoal motility was more frequent after mesterolone treatment than after HCG and PMS or after clomiphene. 14 wives of patients in the mesterolone group became pregnant and gave birth to normal children. There were two pregnancies in the gonadotropin group and 3 in the clomiphene group. Specific treatment of the few patients with varicocele or epididymitis led to improved findings in the spermiogram.
 ...
PMID:The medical treatment of male infertility. 666 60

Attempts were made to elucidate the possible antigonadotropic action of PRL at the ovarian level. Immature female rats of Wistar strain were injected subcutaneously with PMS (5-10 i.u.) at 0900 h on day 23 of age, followed an intraperitoneal injection of HCG (5-10 i.u.) at 1400 h on day 25. In all animals treated with PMS-HCG, ova were found in the oviducts when examined at 0900 h on day 26 of age. Rat PRL was administered either at various doses (1.0-20 micrograms/day) in the morning (0800 h) between day 23-25 or at 5 micrograms/day for 10 days prior to HCG injection. The ability of exogenous gonadotropins to induce ovulation and weight gain of the ovary were not affected by this PRL treatment. In animals injected with PMS, significant rises in serum estradiol-17 beta levels were observed 48 h later. This PMS-induced increase in the estrogen concentration was also not affected by the treatment with various doses of PRL. From these results, it seems likely that exogenously administered PRL may be unable to suppress the ovarian responsiveness to gonadotropins by direct action on the ovary.
 ...
PMID:Lack of inhibitory effect of exogenous prolactin on ovarian responsiveness to gonadotropins in the rat. 688 71

Mouse males aged 2, 4 weeks, and 2 months were given PMS and HCG hormones, and testosterone in intramuscular injections. Leydig cells obtained from treated animals were histochemically investigated for the activity of delta 5, 3 beta-hydroxysteroid dehydrogenase). PMS caused no statistically significant changes in the activity of delta 5, 3 beta-OH-SDH in any of the investigated age groups of mice. However, in all tested groups treated with HCG the amount of cells having very strong enzyme activity increased. Testosterone injected into mice induced decrease of delta 5, 3 beta-OH-SDH activity. Both under the influence of HCG and testosterone differences between control group and experimental ones were statistically significant.
 ...
PMID:Age and hormone dependent activity of Delta5, 3 beta-hydroxysteroid dehydrogenase in isolated Leydig cells from mouse testes. 693 53

The steroid hormone has an important role in the early stages of reproduction. There has been abundant histochemical evidence that oocytes contain steroid hormones and are able to synthesize these hormones. But there have been few methods of analyzing one oocyte biochemically because it is too small and light. In order to study steroidogenesis in the oocyte, a microassay method sensitive enough to analyze the enzyme activities in one oocyte was developed using enzymatic cyling for amplifying the reaction product to 10,000-fold. An oil-well technique and a microtube method were applied in the assay for achieving the reaction in a medium as small as 1.0 to 5.0 microliters under a stereomicroscope. Immature Wistar rats were superovulated by PMS-hCG administration. Oocytes were collected by puncturing the follicle and flushing the tube. They were freeze-dried after washing to remove cumulus cells. The dry weight of one oocyte was 51.2 +/- 6.2 ng in a quartz fiber fishpole balance. The activity of 3 beta hydroxysteroid dehydrogenase (3 beta HSD) (picomol/oocyte/hr, substrate:pregnenolone) in the PMS-treated oocyte was 2.66 +/- 0.59, which corresponds to 3 times the activity of the ovarian homogenate as control, indicating the high capacity of oocytes to produce progesterone. The activity increased significantly (P less than 0.01) by hCG administration up to 4.17 +/- 0.29 after ovulation, suggesting that gonadotropin regulates steroidogenesis in the oocyte. The activities of G6PD and 6PGD were 8.41 +/- 1.09 picomol/oocyte/min and 3.85 +/- 2.02 picomol/oocyte/hr, respectively. The high activity of G6PD (more than 10 times that of the ovarian homogenate) suggests that the pentose phosphate shunt concerned with steroidogenesis is active in the oocyte. HCG decreased the activities of both G6PD and 6PGD. The present results show that steroidogenesis in the oocyte is very active under the control of gonadotropin, suggesting that steroid hormones may play an important role in oocyte maturation, ovulation and fertilization.
 ...
PMID:[Studies on steroidogenesis in the oocyte]. 696 20

A microassay method sensitive enough to analyze the enzyme activities in one oocyte was developed using enzymatic cycling for amplifying the reaction product to 10,000 fold. An oil-well technique was applied in the assay for achieving the reaction in the medium as small as 1.0 to 5.0 microliter. Immature Wistar rats were superovulated by PMS-hCG administration. Oocytes were collected by the puncture of the follicle and the flushing of the tube. They were freeze-dried after washing to remove cumulus cells. The dry weight was about 50ng on a quartz fiber fishpole balance. The activity of hexokinase was 1.75 +/- 0.14 picomol/oocyte/hr corresponding to one-tenth of the ovarian homogenate as control, indicating low capacity of glucose utilization in the oocyte. The activities of G6PD, LDH, and MDH were 8.41 +/- 0.34, 35.7 +/- 2.89. 11.1 +/- 2.5 picomol/oocyte/min, respectively. High activity of G6PD suggests the pentose phosphate shunt concerned with steroidogenesis is active in the oocyte. HCG increased the activities of hexokinase and MDH and decreased that of G6PD. The activity of LDH remained unchanged.
 ...
PMID:[Study of energy metabolism in the oocyte by cycling method]. 717 80

We investigated the effect of exogenous progesterone on follicular growth and pregnant mares' serum gonadotropin and human chorionic gonadotropin (PMS-HCG) induced superovulation in intact and hypophysectomized immature rats. Although the administration of progesterone during PMS-HCG injections had no effect on superovulation, the administration of progesterone beginning 3 days before PMS injection inhibited superovulation in both intact and hypophysectomized rats. In order to study the mechanism of inhibition of superovulation by pretreatment with progesterone, an autoradiographic study using tritiated thymidine was done in hypophysectomized rats just before PMS injection. The labelling indices of granulosa cells were significantly decreased by the administration of progesterone (p < 0.001). Treatment with progesterone resulted in an increase of the follicles in early stages of atresia with pycnotic nuclei in granulosa cells surrounding apparently normal oocytes. Ovarian histology and serum and ovarian tissue concentrations of estradiol were then examined in hypophysectomized rats 20 h after HCG injection. Large Graafian follicles and corpora lutea were rarely seen in rats previously given progesterone, whereas many large Graafian follicles and corpora lutea were observed in the control rats. The concentrations of estradiol in the progesterone-pretreated rats were significantly reduced in serum (p < 0.02) and slightly reduced in ovarian tissues when compared with controls.
 ...
PMID:Inhibitory effect of progesterone on follicular growth and induced superovulation in the rat. 743 55

Four consecutive trials were conducted to investigate the possibility of controlling the time of ovulation in prepuberal gilts pretreated with PMS and HCG. In trial 1 it was shown that the GnRH analog Hoe 766 was superior to other compounds tested. The following trial revealed that 10 mug of that analog is the optimal dose to elicit an ovulatory response. In trial 3 it was found that the majority (73%) of gilts had started ovulating by 39 h after Hoe 766 injection. Individual gilts started ovulating up to 4 h sooner or up to more than 5 h later. Apparently the ovulatory process of an individual gilt extends over a period of 4 - 5 h. Double insemination of 9 gilts at 34 and 41 h after Hoe 766 resulted in fertilization rates and litter sizes that compared favourably with those of corresponding gilts treated with HCG.
 ...
PMID:Induction of ovulation in gonadotropin treated gilts with synthetic gonadotropin releasing hormone. 1672 75


<< Previous 1 2 3 4